Nocardia seriolae, a causative agent of systematic granuloma in spotted butterfish, Scatophagus argus, Linn

Wang, Pei‐Chi; Tsai, Ming‐An; Liang, Yu-Chi; Chan, Y H; Chen, Shih‐Chu

doi:10.5897/ajmr2014.6874

Cited by 18 publications

(6 citation statements)

References 26 publications

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“…The gross and histopathological features observed in this study are typical signs reported in other cases of nocardiosis (Khac et al, 2016;Lewis & Chinabut, 2011;Wang et al, 2014).…”

Section: Discussionsupporting

confidence: 83%

Nocardia seriolae infection in the cultured eel Anguilla japonica in Korea

Kim

Lee

Wan

et al. 2018

Journal of Fish Diseases

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Mass mortality occurred at an Anguilla japonica eel farm equipped with a recirculating aquaculture system in Gimcheon, Korea, from late spring to early summer 2015. The cumulative 3-month mortality was 16% (approximately 24,300-150,000 fish). The majority of affected fish displayed ulcerative lesions that progressed to petechial haemorrhages and small white granulomas in the major organs. A Gram-positive, acid-fast, nonmotile bacterium was isolated from internal organ lesions. Phylogenetic analysis of 16S rRNA identified the species as Nocardia seriolae and the strain was designated EM150506. Afterwards, naïve eels were injected with 1.8 × 10 colony-forming units per fish to confirm the strain's pathogenicity, which resulted in a 20% mortality rate within 4 weeks. However, surviving fish still exhibited white N. seriolae colonies in internal organs. To our knowledge, this is the first report of a N. seriolae infection in cultured eel.

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“…The gross and histopathological features observed in this study are typical signs reported in other cases of nocardiosis (Khac et al, 2016;Lewis & Chinabut, 2011;Wang et al, 2014).…”

Section: Discussionsupporting

confidence: 83%

Nocardia seriolae infection in the cultured eel Anguilla japonica in Korea

Kim

Lee

Wan

et al. 2018

Journal of Fish Diseases

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“…Furthermore, histopathology of typical granulomas -some displaying bacterial colonies-and the organs affected, coincides with previous reports of Nocardia spp. infections in marine fish (Bransden et al, 2000;Wang et al, 2005;Wang et al, 2009;Cornwell et al, 2011;Elkesh et al, 2013;Wang et al, 2014;Vu-Khac et al, 2016). Most of the above-cited reports describe the liver as one of the commonly affected organs however, in our study the liver, was rarely involved.…”

Section: Discussionmentioning

confidence: 43%

“…N. seriolae afflicts mostly marine species. It is a well-known fish pathogen in the Easter Asia region including China, Taiwan and Japan (Wang et al, 2005;Labrie et al, 2008;Wang et al, 2014;Vu-Khac et al, 2016). In 1968 was first isolated from the yellowtail Seriola quinqueradiata and amberjack S. dumerili, (Kariya et al 1968) but it was identified as Nocardia kampachi in posterior discoveries (Ksuda et al 1974).…”

Section: Discussionmentioning

confidence: 99%

“…As a facultative intracellular pathogen, N. seriolae can be a rather elusive. Most of the outbreaks reported in the literature reveal that the bacterium was isolated when major gross signs were displayed (Labrie et al, 2008;Wang et al, 2009;Wang et al, 2014); early stages of the infection seem to pass unnoticed, as the invasion of tissue occurs gradually. This is the reason why nocardiosis is not rapidly detected in aquaculture (Wang et al, 2009;Elkesh et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

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First evidence of nocardiosis in farmed red drum (Sciaenops ocellatus, Linnaeus) caused by Nocardia seriolae in the Gulf of Mexico

Rio-Rodriguez

Ramírez‐Paredes

Soto-Rodriguez

et al. 2021

Preprint

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Between August and December 2013, the offshore cages of a commercial marine farm culturing red drum Sciaenops ocellatus in Campeche Bay were affected by an outbreak of an ulcerative granulomatous disease, reaching 70% cumulative mortality. Thirty-one adults displaying open ulcers on the skin were submitted to our laboratory for diagnosis. Multiple white-yellowish nodules (0.1-0.5 cm in diameter) were present in all internal organs, where the kidney and the spleen were the most severely affected. Histopathological observations of these organs evinced typical granulomatous formations. Gram and Ziehl-Neelsen stains on tissue imprints, bacterial swabs and tissue sections revealed Gram-positive, acid-fast, branching beaded long rod filamentous bacteria. Tissue samples resulted positive for nocardiosis when a Nocardia genus specific nested PCR was used. Definite identification at the species level and taxonomic positioning of the fastidious pathogen was achieved through a specific Nocardia seriolae PCR and by sequencing the gyrB gene of pure isolates. After application of antibiotics during fry production, a posterior follow-up monitoring (from 2014 to 2017) detected mild but recurrent outbreaks of the bacteria with no seasonality pattern. To the extent of our current knowledge, this is the first report of piscine nocardiosis (in a new host -red drum) in Mexico.

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“…Fish nocardiosis is a systemic bacterial disease with three different of pathogenic bacteria, including Nocardia seriolae , N. samonicida and N. asteroides (Chen, ; Pei‐Chi, Ming‐An, Yu‐Chi, Yanting, & Shih‐Chu, ; Xia, Zhang, et al, ). Notably, N. seriolae has been identified as the main pathogen of fish nocardiosis recently (Elkesh, Kantham, Shinn, Crumlish, & Richards, ).…”

Section: Introductionmentioning

confidence: 99%

Identification of a mitochondrial‐targeting secretory protein from Nocardia seriolae which induces apoptosis in fathead minnow cells

Chen

Wang

et al. 2019

Journal of Fish Diseases

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Nocardia seriolae is the main pathogen responsible for fish nocardiosis. A mitochondrial‐targeting secretory protein (MTSP) 3141 with an N‐terminal transit peptide (TP) from N. seriolae was predicted by bioinformatic analysis based on the genomic sequence of the N. seriolae strain ZJ0503. However, the function of the MTSP3141 and its homologs remains totally unknown. In this study, mass spectrometry analysis of the extracellular products from N. seriolae proved that MTSP3141 was a secretory protein, subcellular localization research showed the MTSP3141‐GFP fusion protein co‐localized with mitochondria in fathead minnow (FHM) cells, the TP played an important role in mitochondria targeting, and only the TP located at N‐terminus but not C‐terminus can lead to mitochondria directing. Moreover, quantitative assays of mitochondrial membrane potential (ΔΨm) value, caspase‐3 activity and apoptosis‐related gene (Bcl‐2, Bax, Bad, Bid and p53) mRNA expression suggested that cell apoptosis was induced in FHM cells by the overexpression of both MTSP3141 and MTSP3141ΔTP (with the N‐terminal TP deleted) proteins. Taken together, the results of this study indicated that the MTSP3141 of N. seriolae was a secretory protein, might target mitochondria, induce apoptosis in host cells and function as a virulence factor.

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Nocardia seriolae, a causative agent of systematic granuloma in spotted butterfish, Scatophagus argus, Linn

Cited by 18 publications

References 26 publications

Nocardia seriolae infection in the cultured eel Anguilla japonica in Korea

Nocardia seriolae infection in the cultured eel Anguilla japonica in Korea

First evidence of nocardiosis in farmed red drum (Sciaenops ocellatus, Linnaeus) caused by Nocardia seriolae in the Gulf of Mexico

Identification of a mitochondrial‐targeting secretory protein from Nocardia seriolae which induces apoptosis in fathead minnow cells

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