Nocardia niwae sp. nov., isolated from human pulmonary sources

Abstract

Members of the genus Nocardia are responsible for cutaneous, pulmonary and disseminated human infections. From 2003 to 2008, four nocardioform strains (W8027, W8681, W9071 and W9241 T ) were isolated from patients in the state of Florida, USA. Ribosomal gene sequencing analysis suggested that a novel species of the genus Nocardia had been isolated. These strains were subjected to a taxonomic analysis using a polyphasic approach. Phenotypic analyses included morphological examination, biochemical profiling and antimicrobial susceptibility testing. Molecular studies included 16S rRNA and DNA gyrase B subunit (gyrB) gene sequence analyses and DNA-DNA hybridization. Phylogenetic neighbours were determined through 16S rRNA and gyrB gene sequence analyses. Phenotypic characteristics that differentiated the novel isolates from phylogenetically related species were growth at 45 6C, and three of the four novel strains utilized L-rhamnose. The antimicrobial profiles could not reliably distinguish the novel species from related nocardiae. Analysis showed that the 16S rRNA gene sequences of the four novel isolates were identical. The BLAST

Menace Elbow: Disseminated Nocardiosis

“…The organism was eventually reported as susceptible to linezolid, imipenem, and trimethoprim-sulfamethoxazole. 1 Concern for disseminated nocardiosis prompted chest CT, which disclosed a 14.5-cm cavitary right upper lobe lesion and bilateral patchy opacities but no lymphadenopathy (Figure 3). MRI of the brain demonstrated multiple ring-enhancing lesions (Figure 4).…”

Systemic Nocardia brasiliensis infection in a patient with systemic lupus erythematosus: successful diagnosis and therapy

“…Base sequences determined in the present study were performed by a direct sequencing using an ABI Prism 3130xl sequencer (Applied Biosystems Hitachi, Sangon Biotech Co., Ltd., Shanghai, China) and deposited in the DDBJ database (data not shown). For sequential comparison, 16S rRNA gene sequences were taken from DDBJ database, and all sequence lengths were adjusted to match the length of the shortest sequence (1394 bp) . The sequence homology of 16S rRNA gene (AB427096) of our isolate was 99% with N. brasiliensis (DDBJ ).…”

Identification, Typing, and Phylogenetic Relationships of the Main Clinical Nocardia Species in Spain According to Their gyrB and rpoB Genes

“…To date, nearly 99 Nocardia species have been identified (see NCBI taxonomy for Nocardia, http://www.ncbi.nlm .nih.gov/Taxonomy/Browser/wwwtax.cgi?idϭ1817 and http://www .bacterio.cict.fr/n/nocardia.html), but this number undergoes constant modification. Some one-third of Nocardia species are known human pathogens, and new pathogenic species causing different clinical problems are constantly being discovered (2,3). Infection occurs mainly via the respiratory tract, later disseminating to other locations, such as the central nervous system.…”