The design of high-performance Multiprocessor Systems-on-Chip (MPSoCs) has proven to be an attractive challenge in embedded systems design automation. However, the complexity of such designs associated with short timeto-market constraints impose serious limitations on the exploration of different configurations and scenarios on the design space exploration. The use of virtual platforms may decrease the time-to-market of these architectures while providing the means to exploit, debug and verify architectures with different features. In this paper, we present the webbased Simplify framework, an interactive approach for MPSoC exploration using an instruction-accurate Open Virtual Platform (OVP). The framework provides an environment to define both software and hardware properties in an intuitive way, and allows designers to validate the functionality as well as the behavior of the modeled architectures at highabstraction levels. Based on the simulation reports generated from the framework, designers can perform further design modifications and optimizations, and re-validate the whole system in an efficient way, allowing increased design space exploration. For the evaluation of the proposed approach, a set of benchmark applications extracted from MiBench has been used. They run on five different processors (MIPS32, ARM7, OpenRISC (OR1K), PowerPC32 and MicroBlaze) on both mono and multiprocessor architectures and the experiments show considerable simulation speed-ups to obtain application profiling at instruction-level compared to existing approaches based on tracing.