Nobiletin induces growth inhibition and apoptosis in human nasopharyngeal carcinoma C666‐1 cells through regulating PARP‐2/SIRT1/AMPK signaling pathway

Abstract

Background/Aim Nobiletin, a major polymethoxyflavones ( PMF s) from citri reticulatae pericarpium ( CRP ), can inhibit several forms of cancer proliferation. However, the effects of nobiletin on nasopharyngeal carcinoma ( NPC ) C666‐1 cells remain largely unknown. Materials and Methods Cell counting kit 8 ( CCK 8) assay was used to measure cell vitality. Flow cytometry was performed to measure the apoptosis rate. Quantitative real‐time polymerase chain reaction ( qRT ‐ PCR ) and Western blot analysis were applied to determine the expression of mRNA and protein, respectively. Results We showed that the proliferation rate of C666‐1 cells was inhibited and the apoptosis rate was raised after treating with nobiletin. Moreover, nobiletin inhibited the expression of poly( ADP ‐ribose)polymerase‐2 ( PARP ‐2), and the tumor suppression effect of nobiletin on C666‐1 is associated with PARP ‐2‐dependent pathway. Conclusion We demonstrated for the first time that nobiletin inhibited the growth of C666‐1 cells, which may be relative to its regulation on PARP ‐2/ SIRT 1/ AMPK signaling pathway. Our result implied that nobiletin may serve as a strategy to treat nasopharyngeal carcinoma.

The Role of Citrus Nobiletin on Oxidative Stress Levels and Superoxide Dismutase Activities in Metastatic Castration-Resistant Prostate Cancer

“…Nobiletin (NOB) is a bioactive polymethoxylated flavone (5,6,7,8,3',4'-hexamethoxyflavone) which was found in citrus peels and had multiple biologic activities (Bernini et al, 2011;Zheng et al, 2019;Dusabimana et al, 2019). Flavonoids exhibit anti-carcinogenic characteristics in vitro and reduce cancer risk by protecting cells from oxidation or inflammation by changing the level of sex hormones, reducing angiogenesis or cell proliferation, and regulating apoptosis (Gates et al, 2009).…”

Evaluation of dynamic changes in the bioactive components in Citri Reticulatae Pericarpium (Citrus reticulata ‘Chachi’) under different harvesting and drying conditions

“…Flavonoids like hesperidin and polymethoxyflavones (PMFs) like nobiletin and tangeretin are the major bioactive compounds, providing antitumor, antioxidant and anti-neuroinflammatory properties. [3][4][5] Small quantities of alkaloids, phenolic acids and limonoids have been detected in CRCP, where these compounds are widely known to possess anti-asthmatic, antibacterial and antitumor activity, respectively. [6][7][8] Volatile oil is a secondary metabolite in CRCP that confers an aromatic odor.…”

Identification of lipophilic components in Citri Reticulatae Pericarpium cultivars by supercritical CO2 fluid extraction with ultra‐high‐performance liquid chromatography–Q Exactive Orbitrap tandem mass spectrometry

“…Citri Reticulatae Pericarpium (CRP), the desiccated mature peel of Citrus reticulata Blanco or its cultivated varieties, is a traditional Chinese medicine with extensive effects [1]. Until now, most studies have shown that the pharmacological activities of CRP include anti‐asthmatic effects, anti‐neuroinflammatory activity, antioxidant ability, and anti‐cancer activity [2–5]. However, C. reticulata Blanco comprises a variety of cultivars, including four main cultivars (“Chachi,” “Tangerina,” “Dahongpao,” and “Unshiu”) and others (“Shiyueju,” “Ponkan,” “Kinokuni,” “Yichangju,” etc.).…”