NO trapping in biological systems with a functionalized zeolite network

Electron spin resonance

“…The sensitivity of the methods is superior to using free iron complexes in solution, and it is proposed that the material might be anchored to solid surfaces, mixed into a composite or pressed into small pellets. 46 The yield of nitric oxide produced from 1 mM sodium nitrite solution varies by a factor of 200 when the reaction was initiated by 10 mM sodium dithionite in the presence of 5 or 150 mM HEPES-buffer (at pH 7.4). The amount of nitric oxide was estimated by the ESR as incorporated into water-soluble complexes of Fe with N-methyl-D-glucamine dithiocarbamate (MGD).…”

A nitric oxide quantitative assay by a glyceraldehyde 3-phosphate dehydrogenase/phosphoglycerate kinase/firefly luciferase optimized coupled bioluminescent assay

“…Although simple to perform, this methodology may not reect the endogenous cNO content, which originates from nitric oxide synthase reactions with the amino acid L-arginine, 2 because other sources may affect the results, for example dietary intake. 6 Other popular methods are based on uorescence, 7 ultraviolet-visible (UV-vis) spectrophotometry, 8 electrochemical sensors, [9][10][11] chemiluminescence with ozone, 12,13 spin trapping coupled to electron paramagnetic resonance (EPR) spectroscopy 14,15 and membrane inlet mass spectrometry (MS). 16 Recent approaches for cNO quantitation include the use of nanomaterials to create novel electrochemical sensors, [17][18][19][20][21] the synthesis of novel uorescent probes, 22,23 the bioimaging of cNO in living cells and small animals 23,24 and coupled enzymatic assays.…”

Efeito dos sulfactantes sobre o sensor sólido de óxido nítrico preparado pelo processo Sol-Gel

“…A figura 27 mostra o sinal RPE do complexo NO-Fe Sensores no estado sólido, utilizando matrizes porosas, para identificação e quantificação do NO, à temperatura ambiente, pode-se dizer que é algo inédito, havendo na literatura apenas um trabalho semelhante, utilizando matrizes de zeólita [106 ]. Dessa forma, a proposta de um sensor sólido de NO, pioneira na quantificação de NO através de materiais porosos utilizando a técnica de RPE, associada ao aprisionamento de spins, alia uma série de vantagens, como descrito a seguir: -DETC seja depositada na ponta de uma fibra ótica, a mesma torna-se a um sensor semelhante aos microeletrodos utilizados em eletroquímica, com as vantagens já mencionadas.…”