No mitigation of IFN-β and HLA class I expression in early sub-cultured human neonatal skin fibroblasts but both molecules are overexpressed in starved cells

Proteome analysis, bioinformatic prediction and experimental evidence revealed immune response down-regulation function for serum-starved human fibroblasts

“…To our knowledge there is no report in literatures about the expression of this molecule in starved fibroblasts. However, we have already reported that during serum starvation stress, foreskin originated fibroblasts were able to up-regulate the HLA-class I expression [ 67 ]. Moreover, as it has already been described, to predict the biological roles of each proteome, in silico analysis was performed.…”

“…We found a significant reduction in proliferation of alloreactive lymphocytes which were incubated for three days with starved fibroblasts compared to the cells stimulated with non-starved fibroblasts control, P < 0.01. We have already shown that the HLA-class I expression was increased in human fibroblasts [ 67 ] whereas it was decreased in PBMCs [ 79 ], in a time dependent manner. Here we supposed that the over expression of classical HLA-class I or probably non -classic HLA-G induced such significant immunosuppression.…”