No miRNA were found in Plasmodium and the ones identified in erythrocytes could not be correlated with infection

Xue, Xiangyang; Zhang, Qingfeng; Huang, Yufu; Li, Feng; Pan, Weiqing

doi:10.1186/1475-2875-7-47

Cited by 80 publications

(72 citation statements)

References 23 publications

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“…2) and it is known that plasmodia do not express miRNA (72). Mice inoculated with PbA-infected erythrocytes became moribund on day 7, displaying neurological signs, including partial paralysis, seizures, and coma.…”

Section: Resultsmentioning

confidence: 99%

Differential MicroRNA Expression in Experimental Cerebral and Noncerebral Malaria

et al. 2011

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MicroRNAs (miRNAs) are posttranscriptional regulatory molecules that have been implicated in the regulation of immune responses, but their role in the immune response to Plasmodium infection is unknown. We studied the expression of selected miRNAs following infection of CBA mice with Plasmodium berghei ANKA (PbA), which causes cerebral malaria (CM), or Plasmodium berghei K173 (PbK), which causes severe malaria but without cerebral complications, termed non-CM. The differential expression profiles of selected miRNAs (let-7i, miR-27a, miR-150, miR-126, miR-210, and miR-155) were analyzed in mouse brain and heart tissue by quantitative reverse transcription-PCR (qRT-PCR). We identified three miRNAs that were differentially expressed in the brain of PbA-infected CBA mice: let7i, miR-27a, and miR-150. In contrast, no miRNA changes were detected in the heart, an organ with no known pathology during acute malaria. To investigate the involvement of let-7i, miR-27a, and miR-150 in CM-resistant mice, we assessed the expression levels in gamma interferon knockout (IFN-␥ ؊/؊ ) mice on a C57BL/6 genetic background. The expression of let-7i, miR-27a, and miR-150 was unchanged in both wild-type (WT) and IFN-␥ ؊/؊ mice following infection. Overexpression of these three miRNAs during PbA, but not PbK, infection in WT mice may be critical for the triggering of the neurological syndrome via regulation of their potential downstream targets. Our data suggest that in the CBA mouse at least, miRNA may have a regulatory role in the pathogenesis of severe malaria.

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Section: Resultsmentioning

confidence: 99%

Differential MicroRNA Expression in Experimental Cerebral and Noncerebral Malaria

et al. 2011

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“…In protozoa, however, the presence of miRNAs is still uncertain. It has been reported that miRNA-like molecules could be identified in some species of protozoa (Saraiya and Wang 2008;Braun et al 2010;Huang et al 2012), but many studies reported that the conserved miRNA or miRNA-like molecules could not be found in the protozoa examined (Grimson et al 2008;Xue et al 2008;Atayde et al 2011). In contrast, 5 These authors contributed equally to this work.…”

Section: Introductionmentioning

confidence: 95%

Comparative transcriptome analysis of small noncoding RNAs in different stages of Trypanosoma brucei

Zheng

Wen

Yang

et al. 2013

RNA

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Trypanosoma brucei, a pathogen of human and domestic animals, is an early evolved parasitic protozoan with a complex life cycle. Most genes of this parasite are post-transcriptionally regulated. However, the mechanisms and the molecules involved remain largely unknown. We have deep-sequenced the small RNAs of two life stages of this parasite-the bloodstream form and the procyclic form. Our results show that the small RNAs of T. brucei could derive from multiple sources, including NATs (natural antisense transcripts), tRNAs, and rRNAs. Most of these small RNAs in the two stages were found to share uniform characteristics. However, our results demonstrate that their variety and expression show significant differences between different stages, indicating possible functional differentiation. Dicer-knockdown evidence further proved that some of the small interfering RNAs (siRNAs) could regulate the expression of genes. Based on the genome-wide analysis of the small RNAs in the two stages of T. brucei, our results not only provide evidence to study their differentiation but also shed light on questions regarding the origins and evolution of small RNA-based mechanisms in early eukaryotes.

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“…However, for each others' species, this regulation is more complicated. Previous studies have shown that the malaria parasite does not encode miRNAs, 28 yet these pathogens might encode other small non-coding RNAs. We propose that Plasmodium regulates gene expression in host cells through small non-coding RNAs.…”

Section: Resultsmentioning

confidence: 99%

New roles for microRNAs in cross-species communication

et al. 2013

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No miRNA were found in Plasmodium and the ones identified in erythrocytes could not be correlated with infection

Cited by 80 publications

References 23 publications

Differential MicroRNA Expression in Experimental Cerebral and Noncerebral Malaria

Differential MicroRNA Expression in Experimental Cerebral and Noncerebral Malaria

Comparative transcriptome analysis of small noncoding RNAs in different stages of Trypanosoma brucei

New roles for microRNAs in cross-species communication

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