No increase in micronuclei frequency in cultured blood lymphocytes from a group of filling station attendants

Pitarque, Marià; Carbonell, E.; Lapeña, N.; Marsá, M.; Torres, Miquel; Creus, A.; Xamena, N.; Marcos, Ricard

doi:10.1016/0165-1218(95)00091-7

Cited by 47 publications

(14 citation statements)

References 22 publications

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“…No correlation was found between smoking habits and BNMN frequency in our population, which is also in agreement with other published reports [Pitarque et al, 1996;Bukvic et al, 2001;Bonassi et al, 2003]. …”

Section: Discussionsupporting

confidence: 95%

Cytogenetic damage in female Chilean agricultural workers exposed to mixtures of pesticides

Márquez

Villalobos

Poblete

et al. 2004

Environ. Mol. Mutagen.

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The VIII Region of Bio-Bio is a major fruit-growing area of Chile that makes intensive use of agricultural pesticides. The cytogenetic damage associated with exposure to mixtures of pesticides was evaluated by comparing peripheral blood lymphocyte micronucleus (MN) frequencies in a group of 64 female agricultural workers and 30 female controls. The exposed subjects worked during the spring and summer in thinning and pruning fruit trees and in harvesting and packing different fruits, such as raspberries, grapes, apples, and kiwis. They did not use any protective measures during their work activities. A significant increase in the frequency of binucleated cells with micronuclei (BNMN) was found in the exposed women as compared with the controls (36.94 +/- 14.47 vs. 9.93 +/- 6.17 BNMN/1000 BN cells; P < 0.001). The frequency of BNMN varied as a function of age in both the exposed and control groups, but no correlation was found between BNMN frequency and the duration of exposure. Also, smoking and other habits had no effect on MN frequency. Our study confirms that occupational exposure to pesticide mixtures results in cytogenetic damage.

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Section: Discussionsupporting

confidence: 95%

Cytogenetic damage in female Chilean agricultural workers exposed to mixtures of pesticides

Márquez

Villalobos

Poblete

et al. 2004

Environ. Mol. Mutagen.

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“…The results indicated no exposure-related excess of MN in traffic policemen compared to indoor workers, despite the significantly higher exposure to benzene and other pollutants among the traffic policemen. Similar results were reported from other investigations on workers exposed to traffic exhaust and petroleum fuels [Carere et al, 1995;Pitarque et al, 1996;Bolognesi et al, 1997;Parry et al, 1997]. These results suggest that the CBMN assay may lack the sensitivity required to detect the effects produced by low-level exposures to genotoxic agents and/or that this particular exposure to environmental pollutants is devoid of significant consequences on DNA integrity.…”

Section: Discussionsupporting

confidence: 89%

Analysis of micronuclei in peripheral blood lymphocytes of traffic wardens: Effects of exposure, metabolic genotypes, and inhibition of excision repair in vitro by ARA‐C

Leopardi

Zijno

Marcon

et al. 2003

Environ and Mol Mutagen

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The cytokinesis-block micronucleus (MN) assay in peripheral lymphocytes was used to assess the genetic effects of the occupational exposure to traffic fumes in policemen from the Municipality of Rome. The study population consisted of 192 subjects engaged in traffic control (exposed, 134 subjects), or in office work (controls, 58 subjects). Groups were balanced for age, gender, and smoking habits. The average benzene exposure during the workshift was 9.5 and 3.8 microg/m(3) in exposed individuals and controls, respectively. All subjects were genotyped for CYP1A1, CYP2E1, GSTM1, GSTT1, and DT-diaphorase polymorphisms. The incidence of micronuclei and micronucleated cells was recorded in 1,000 binucleated cells harvested 66 hr after mitogen stimulation. Regression analysis of data showed that MN frequency was mainly modulated by the age (P = 0.001) and gender (P = 0.001) of the study subjects (relatively higher in the elderly and females), whereas it was unaffected by the occupational exposure to traffic fumes and smoking habits. A weak (P = 0.02) association between lower MN frequency and the GSTM1 null genotype was also observed. In order to improve the sensitivity of the method to excision-repairable lesions, a modified protocol, with exposure of cells to the repair inhibitor cytosine arabinoside (Ara-C) during the first 16 hr of growth, was applied to 78 subjects (46 exposed and 32 controls). The results confirmed the higher MN frequency in females (P < 0.05), but failed to demonstrate any significant effect of chemical exposure (occupational or related to smoking habits). When the frequency of MN induced by Ara-C (i.e., spontaneous values subtracted) was considered, a significant inverse correlation with age was observed (P = 0.005), possibly related to the age-dependent decrease in repair proficiency.

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“…The most plausible interpretations for the lack of such effects might be that VCM exposure was likely to mask the effect of smoking or alcohol drinking or that there was lower effective concentration of cigarette smoke chemical or alcohol chemical in the blood which could cause chromosomal damage in lymphocytes. 42,43 In summary, our results indicated that TDG Gly199Ser polymorphism was related to micronucleus frequency in VCM-exposed workers and APE1 and TDG functioned in a coordinated manner. The strengths of this study include a homogeneous ethnic background of VCM-exposed workers, reduction in information bias by performing direct interviews in collecting demographic and lifestyle information, and well-documented exposure history in the workplace.…”

Section: Discussionmentioning

confidence: 76%

Micronucleus Occurrence Related to Base Excision Repair Gene Polymorphisms in Chinese Workers Occupationally Exposed to Vinyl Chloride Monomer

Wang

Qiu

et al. 2009

Journal of Occupational &Amp; Environmental Medicine

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No increase in micronuclei frequency in cultured blood lymphocytes from a group of filling station attendants

Cited by 47 publications

References 22 publications

Cytogenetic damage in female Chilean agricultural workers exposed to mixtures of pesticides

Cytogenetic damage in female Chilean agricultural workers exposed to mixtures of pesticides

Analysis of micronuclei in peripheral blood lymphocytes of traffic wardens: Effects of exposure, metabolic genotypes, and inhibition of excision repair in vitro by ARA‐C

Micronucleus Occurrence Related to Base Excision Repair Gene Polymorphisms in Chinese Workers Occupationally Exposed to Vinyl Chloride Monomer

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