No evidence of seed transmissibility of Tomato yellow leaf curl virus in Nicotiana benthamiana

Rosas-Díaz, Tábata; Zhang, Dan; Lozano‐Durán, Rosa

doi:10.1631/jzus.b1600463

Cited by 13 publications

(15 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…There are conflicting reports about the seed transmission of TYLCV to other hosts. While it has been reported that TYLCV is seed-transmissible in soybean ( Glycine max ) [27], no evidence of seed transmissibility of TYLCV in the experimental host Nicotiana benthamiana was found [28]. In the present study, BCTV-Svr was detected in seeds of naturally infected petunia plants (local cultivar) and their progeny plants, by molecular and serological tests.…”

Section: Discussioncontrasting

confidence: 57%

Seed Transmission of Beet Curly Top Virus and Beet Curly Top Iran Virus in a Local Cultivar of Petunia in Iran

Anabestani

Behjatnia

Izadpanah

et al. 2017

Viruses

View full text Add to dashboard Cite

Beet curly top virus (BCTV) and beet curly top Iran virus (BCTIV) are known as the causal agents of curly top disease in beet and several other dicotyledonous plants in Iran. These viruses are transmitted by Circulifer species, and until now, there has been no confirmed report of their seed transmission. A percentage (38.2–78.0%) of the seedlings developed from the seeds of a petunia local cultivar under insect-free conditions showed stunting, interveinal chlorosis, leaf curling, and vein swelling symptoms, and were infected by BCTV when tested by PCR. Presence of BCTV in seed extracts of petunia local cultivar was confirmed by PCR and IC-PCR, followed by sequencing. Agroinoculation of curly top free petunia plants with a BCTV infectious clone resulted in BCTV infection of plants and their developed seeds. These results show the seed infection and transmission of BCTV in a local cultivar of petunia. Similar experiments performed with BCTIV showed that this virus is also seed transmissible in the same cultivar of petunia, although with a lower rate (8.8–18.5%). Seed transmission of curly top viruses may have significant implications in the epidemiology of these viruses.

show abstract

Section: Discussioncontrasting

confidence: 57%

Seed Transmission of Beet Curly Top Virus and Beet Curly Top Iran Virus in a Local Cultivar of Petunia in Iran

Anabestani

Behjatnia

Izadpanah

et al. 2017

Viruses

View full text Add to dashboard Cite

show abstract

“…In conclusion, our data, derived from large-scale seedling grow-out and testing of 23,034 seedlings of 118 maternal genotype entries, dehisced seed coat and cotyledon tests, and vector transmission tests, do not support seed transmission of SPLCV. These results provide additional evidence disputing the seed transmission of begomoviruses [ 32 , 33 ]. With efficient whitefly transmission of begomoviruses, we recommend re-examination of seed transmission claims for this group of vector-transmitted plant viruses.…”

Section: Discussionmentioning

confidence: 79%

“…Kothandaraman et al [ 31 ] reported the detection of mung bean yellow mosaic virus (MYMV) in seed coat, cotyledon, and embryonic axes, but no positive infection in grow-out seedlings, emphasizing the seedborne nature of the begomovirus. No evidence of seed transmission of TYLCV was detected in Nicotiana benthamiana [ 32 ]. Further investigation into the seedborne nature and possible seed transmission of TYLCV-IL in a large-scale grow-out experiment on seven tomato genotypes by Pérez-Padilla et al [ 33 ] confirmed that the virus can be detected on and in tomato seeds, including the embryo, but that seed-to-seedling virus transmission did not occur, concluding that seed transmission is not a general property of TYLCV-IL.…”

Section: Introductionmentioning

confidence: 99%

Large-Scale Seedling Grow-Out Experiments Do Not Support Seed Transmission of Sweet Potato Leaf Curl Virus in Sweet Potato

Andreason

Olaniyi

Gilliard

et al. 2021

Plants

View full text Add to dashboard Cite

Sweet potato leaf curl virus (SPLCV) threatens global sweet potato production. SPLCV is transmitted by Bemisia tabaci or via infected vegetative planting materials; however, SPLCV was suggested to be seed transmissible, which is a characteristic that is disputed for geminiviruses. The objective of this study was to revisit the validity of seed transmission of SPLCV in sweet potato. Using large-scale grow-out of sweet potato seedlings from SPLCV-contaminated seeds over 4 consecutive years, approximately 23,034 sweet potato seedlings of 118 genotype entries were evaluated. All seedlings germinating in a greenhouse under insect-proof conditions or in a growth chamber were free of SPLCV; however, a few seedlings grown in an open bench greenhouse lacking insect exclusion tested positive for SPLCV. Inspection of these seedlings revealed that B. tabaci had infiltrated the greenhouse. Therefore, transmission experiments were conducted using B. tabaci MEAM1, demonstrating successful vector transmission of SPLCV to sweet potato. Additionally, tests on contaminated seed coats and germinating cotyledons demonstrated that SPLCV contaminated a high percentage of seed coats collected from infected maternal plants, but SPLCV was never detected in emerging cotyledons. Based on the results of grow-out experiments, seed coat and cotyledon tests, and vector transmission experiments, we conclude that SPLCV is not seed transmitted in sweet potato.

show abstract

“…Revista Mexicana de FITOPATOLOGÍA Mexican Journal of Phytopathology semilla de Solanum lycopersicum (Kil et al, 2016) pero no de Nicotiana benthamiana (Rosas-Díaz et al, 2017). Lo anterior podría explicar la detección de OYMMV en semillas de malezas pero no en semillas de jamaica.…”

Section: Fully Bilingualunclassified

Evaluación de la transmisión de begomovirus por semilla de jamaica y malezas asociadas

Ortega-Acosta

Ochoa-Martínez

Hérnández-Morales

et al. 2018

RMF

View full text Add to dashboard Cite

En el estado de Guerrero, México, los begomovirus Okra yellow mosaic Mexico virus (OYMMV) y Whitefly-asssociated begomovirus 3 (WfaBV3) se han encontrado asociados al cultivo de jamaica (Hibiscus sabdariffa L.). El objetivo de este estudio fue conocer si OYMMV y WfaBV3 pueden infectar malezas presentes en cultivos comerciales de esta especie y si se transmiten por semilla. En 2016, se recolectaron plantas de jamaica y malezas con síntomas de amarillamiento y mosaico en Guerrero. Se extrajo DNA total y se realizó PCR con iniciadores universales para begomovirus y específicos para el gen 18s rRNA como control interno. Las muestras positivas se analizaron posteriormente con iniciadores específicos para OYMMV y WfaBV3. Cien semillas de jamaica y de malezas que presentaban síntomas se analizaron por PCR para begomovirus; cien semillas de cada cultivar de jamaica y 40 semillas de cada maleza se sembraron en almácigos para obtener plántulas. Hojas jóvenes de las plántulas de jamaica se analizaron por PCR después de la amplificación de DNA por círculo rodante. En plantas maduras de jamaica se detectó a WfaBV3 y OYMMV. En Sida collina, S. aggregata, S. acuta, S. haenkeana y Malacra fasciata se detectó al OYMMV en planta madura, semilla y plántulas. Este es el primer reporte de cuatro nuevos hospedantes del OYMMV y su transmisión por semilla.

show abstract

No evidence of seed transmissibility of Tomato yellow leaf curl virus in Nicotiana benthamiana

Cited by 13 publications

References 5 publications

Seed Transmission of Beet Curly Top Virus and Beet Curly Top Iran Virus in a Local Cultivar of Petunia in Iran

Seed Transmission of Beet Curly Top Virus and Beet Curly Top Iran Virus in a Local Cultivar of Petunia in Iran

Large-Scale Seedling Grow-Out Experiments Do Not Support Seed Transmission of Sweet Potato Leaf Curl Virus in Sweet Potato

Evaluación de la transmisión de begomovirus por semilla de jamaica y malezas asociadas

Contact Info

Product

Resources

About