No evidence for genetic differentiation in juvenile traits between Belgian and French populations of the invasive tree Robinia pseudoacacia

Bouteiller, Xavier; Barraquand, Frédéric; Garnier‐géré, Pauline; Harmand, Noémie; Laizet, Yec’han; Raimbault, Alexandre; Segura, Raphael; Lassois, Ludivine; Monty, Arnaud; Verdu, Cindy; Mariette, Stéphanie; Porté, Annabel J.

doi:10.5091/plecevo.2018.1403

Cited by 6 publications

(11 citation statements)

References 60 publications

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“…Two genotyping experiments were performed using these SNPs: (a) All collected samples (initial dataset) were genotyped using 113 SNPs, which were selected according to the procedure presented in Bouteiller et al (); (b) after clone removal (see Section 3), 163 individuals were subsampled randomly within the populations in each range to maintain the sampling design between ranges (additional dataset: 69 individuals from the USA and 96 from Europe) and genotyped using a total of 251 SNPs. These additional SNPs were among the SNPs developed by Verdu et al (), which had first been discarded as we had initially chosen to prioritize the more polymorphic SNPs.…”

Section: Methodsmentioning

confidence: 99%

“…SNP genotyping was performed using the Sequenom MassARRAY System (Agena Bioscience, San Diego, USA) at the Bordeaux Genome Transcriptome Facility (https://pgtb.cgfb.u-bordeaux.fr/en), and using the iPLEX Gold chemistry genotyping kit according to the manufacturer's instructions. SNP data were visualized and validated using ViClust, a R program that we implemented for Galaxy (https://usegalaxy.org/); the program was also made available as a standalone R script for Linux or Windows (Bouteiller et al, ). The whole dataset will be made available on the Open Science Framework repository after acceptation.…”

Section: Methodsmentioning

confidence: 99%

“…The plant material was then crushed using an automated grinder (2010 Geno/Grinder, SPEX SamplePrep, Metuchen, NJ, USA). For four populations (Corphalie, Drewnica, Pinczow and Lewisburg), a few seeds from ten sampled mother trees were scarified and grown in the laboratory (Bouteiller, Porté, Mariette, & Monty, 2017 (Bouteiller et al, 2018).…”

Section: Dna Extraction and Genotypingmentioning

confidence: 99%

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A few north Appalachian populations are the source of European black locust

Bouteiller

Verdu

Aikio

et al. 2019

Ecology and Evolution

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The role of evolution in biological invasion studies is often overlooked. In order to evaluate the evolutionary mechanisms behind invasiveness, it is crucial to identify the source populations of the introduction. Studies in population genetics were carried out on Robinia pseudoacacia L., a North American tree which is now one of the worst invasive tree species in Europe. We realized large‐scale sampling in both the invasive and native ranges: 63 populations were sampled and 818 individuals were genotyped using 113 SNPs. We identified clonal genotypes in each population and analyzed between and within range population structure, and then, we compared genetic diversity between ranges, enlarging the number of SNPs to mitigate the ascertainment bias. First, we demonstrated that European black locust was introduced from just a limited number of populations located in the Appalachian Mountains, which is in agreement with the historical documents briefly reviewed in this study. Within America, population structure reflected the effects of long‐term processes, whereas in Europe it was largely impacted by human activities. Second, we showed that there is a genetic bottleneck between the ranges with a decrease in allelic richness and total number of alleles in Europe. Lastly, we found more clonality within European populations. Black locust became invasive in Europe despite being introduced from a reduced part of its native distribution. Our results suggest that human activity, such as breeding programs in Europe and the seed trade throughout the introduced range, had a major role in promoting invasion; therefore, the introduction of the missing American genetic cluster to Europe should be avoided.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Dna Extraction and Genotypingmentioning

confidence: 99%

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A few north Appalachian populations are the source of European black locust

Bouteiller

Verdu

Aikio

et al. 2019

Ecology and Evolution

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“…Studying the phenotypic variation in germination and juvenile traits in invasive black locust populations from Belgium and France revealed a very high percentage germination, with no sign of inter-population genetic differentiation. Furthermore, all the studied traits showed high plasticity to temperature, with a higher percentage germination when seedlings were grown at 22 °C compared to 18 °C (Bouteiller et al 2018). In the present study, germination-related traits were investigated by extending the sampling area to both the native and invasive ranges.…”

Section: Introductionmentioning

confidence: 93%

The seeds of invasion: enhanced germination in invasive European populations of black locust (Robinia pseudoacacia L.) compared to native American populations

et al. 2021

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Local adaptation and the evolution of phenotypic plasticity may facilitate biological invasions. Both processes can enhance germination and seedling recruitment, which are crucial life-history traits for plants. The rate, timing and speed of germination have recently been documented as playing a major role during the invasion process. Black locust (Robinia pseudoacacia L.) is a North American tree, which has spread widely throughout Europe. A recent study demonstrated that a few populations are the source of European black locust. Thus, invasive populations can be compared to native ones in order to identify genetic-based phenotypic differentiation and the role of phenotypic plasticity can thereby be assessed.• A quantitative genetics experiment was performed to evaluate 13 juvenile traits of both native and invasive black locust populations (3000 seeds, 20 populations) subjected to three different thermal treatments (18 °C, 22 °C and 31 °C).• The results revealed European populations to have a higher germination rate than the native American populations (88% versus 60%), and even when genetic distance between populations was considered. Moreover, this trait showed lower plasticity to temperature in the invasive range than in the native one. Conversely, other studied traits showed high plasticity to temperature, but they responded in a similar way to temperature increase: the warmer the temperature, the higher the growth rate or germination traits values.• The demonstrated genetic differentiation between native and invasive populations testifies to a shift between ranges for the maximum germination percentage. This pattern could be due to human-mediated introduction of black locust.

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“…The 160 SNPs were arranged in four multiplexes of 40 loci. The SNP data were visualized and validated using the R program viclust (Bouteiller et al, 2018; see also https://github.com/ garni erger e/ViClust).…”

Section: Snp Selection Genotyping and Validationmentioning

confidence: 99%

Candidate gene SNP variation in floodplain populations of pedunculate oak (Quercus robur L.) near the species' southern range margin: Weak differentiation yet distinct associations with water availability

et al. 2020

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Populations residing near species' low‐latitude range margins (LLMs) often occur in warmer and drier environments than those in the core range. Thus, their genetic composition could be shaped by climatic drivers that differ from those occurring at higher latitudes, resulting in potentially adaptive variants of conservation value. Such variants could facilitate the adaptation of populations from other portions of the geographical range to similar future conditions anticipated under ongoing climate change. However, very few studies have assessed standing genetic variation at potentially adaptive loci in natural LLM populations. We investigated standing genetic variation at single nucleotide polymorphisms (SNPs) located within 117 candidate genes and its links to putative climatic selection pressures across 19 pedunculate oak (Quercus robur L.) populations distributed along a regional climatic gradient near the species' southern range margin in southeastern Europe. These populations are restricted to floodplain forests along large lowland rivers, whose hydric regime is undergoing significant shifts under modern rapid climate change. The populations showed very weak geographical structure, suggesting extensive genetic connectivity and gene flow or shared ancestry. We identified eight (6.2%) positive FST‐outlier loci, and genotype–environment association analyses revealed consistent associations between SNP allele frequencies and several climatic variables linked to water availability. A total of 61 associations involving 37 SNPs (28.5%) from 35 annotated genes provided important insights into putative functional mechanisms in our system. Our findings provide empirical support for the role of LLM populations as sources of potentially adaptive variation that could enhance species’ resilience to climate change‐related pressures.

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No evidence for genetic differentiation in juvenile traits between Belgian and French populations of the invasive tree Robinia pseudoacacia

Cited by 6 publications

References 60 publications

A few north Appalachian populations are the source of European black locust

A few north Appalachian populations are the source of European black locust

The seeds of invasion: enhanced germination in invasive European populations of black locust (Robinia pseudoacacia L.) compared to native American populations

Candidate gene SNP variation in floodplain populations of pedunculate oak (Quercus robur L.) near the species' southern range margin: Weak differentiation yet distinct associations with water availability

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