No estrogen-like effects of an isopropanolic extract of Rhizoma Cimicifugae racemosae on uterus and vena cava of rats after 17 day treatment

Kretzschmar, Georg; Nisslein, Thomas; Zierau, Oliver; Vollmer, Günter

doi:10.1016/j.jsbmb.2005.05.008

Cited by 25 publications

(18 citation statements)

References 23 publications

(21 reference statements)

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“…Most of the published results on this topic have been obtained in ovariectomized rats treated up to 3 months with C. racemosa extracts [11,23]. The immature uterotrophic assay we used, more sensitive than the ovariectomized uterotrophic assay [24], confirmed that with black cohosh this effect is absent.…”

Section: Discussionsupporting

confidence: 60%

Estrogen-like effect of a Cimicifuga racemosa extract sub-fraction as assessed by in vivo, ex vivo and in vitro assays

Bolle

Mastrangelo

Perrone

et al. 2007

The Journal of Steroid Biochemistry and Molecular Biology

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Section: Discussionsupporting

confidence: 60%

Estrogen-like effect of a Cimicifuga racemosa extract sub-fraction as assessed by in vivo, ex vivo and in vitro assays

Bolle

Mastrangelo

Perrone

et al. 2007

The Journal of Steroid Biochemistry and Molecular Biology

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“…The C3 promoter region contains three estrogen responsive elements making it a highly estrogen sensitive gene in the rat uterus [9]. Because of this high sensitivity the low levels of estrogens still present in the body after ovariectomy seem to be sufficient to maintain an elevated baseline level of C3 mRNA expression, which could be downregulated by 7-O-PN, a feature previously detected for the pure antiestrogen fulvestrant in a 17-day uterotrophic assay [19].…”

Section: Discussionmentioning

confidence: 90%

Effects of the chemically synthesized flavanone 7-(O-prenyl)naringenin-4′-acetate on the estrogen signaling pathway in vivo and in vitro

Kretzschmar

Vollmer

Schwab

et al. 2007

The Journal of Steroid Biochemistry and Molecular Biology

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“…MMLV reverse transcriptase (Life Technologies) and random hexamers were used for the first-strand cDNA synthesis. qRT-PCR analysis was performed in three independent runs as described elsewhere using 18SrRNA as an endogenous reference and SybrGreen1 to detect PCR products (Kretzschmar et al 2005). The amount of cDNA per PCR reaction corresponded to 65 ng of total RNA.…”

Section: Rna Preparation and Quantitative Real-time Pcr (Qrt-pcr)mentioning

confidence: 99%

The social status of the male Nile tilapia (Oreochromis niloticus) influences testis structure and gene expression

Pfennig¹,

Kurth²,

Meißner³

et al. 2012

Reproduction

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Dominant and territorial behaviour are known social phenomena in cichlids and social stress influences reproduction and growth. The gonadotropic hormones trigger spermatogenesis and subordinate males have typically lower levels of gonadotropins than dominant males. In this study, we compared testis morphology and gene expression of dominant and subordinate Nile tilapia males (d-and s-males) in socially stable communities. The d-males had the highest gonadosomatic index but they were not the largest animals in the majority of studied cases. Long-term d-males showed large groups of Leydig cells and hyperplasia of the tunica albuginea due to numerous cytochrome-P450-11b-hydroxylase (Cyp11b) expressing myoid cells. Increased Cyp11b expression in d-males was reflected by elevated 11-ketotestosterone plasma values. However, immunofluorescence microscopy and expression analysis of selected genes revealed that most s-males conserved their capability for spermatogenesis and are, therefore, ready for reproduction when the social environment changes. Moreover, in s-males gene expression analysis by quantitative RT-PCR showed increased transcript levels for germ line-specific genes (vasa, sox2 and dmc1) and Sertoli-specific genes (amh, amhrII and dmrt1) whereas gene expression of key factors for steroid production (sf1 and cyp11b) were reduced. The Nile tilapia is a promising model to study social cues and gonadotropic signals on testis development in vertebrates.

show abstract

No estrogen-like effects of an isopropanolic extract of Rhizoma Cimicifugae racemosae on uterus and vena cava of rats after 17 day treatment

Cited by 25 publications

References 23 publications

Estrogen-like effect of a Cimicifuga racemosa extract sub-fraction as assessed by in vivo, ex vivo and in vitro assays

Estrogen-like effect of a Cimicifuga racemosa extract sub-fraction as assessed by in vivo, ex vivo and in vitro assays

Effects of the chemically synthesized flavanone 7-(O-prenyl)naringenin-4′-acetate on the estrogen signaling pathway in vivo and in vitro

The social status of the male Nile tilapia (Oreochromis niloticus) influences testis structure and gene expression

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