No detection of EBV, BKV and JCV in breast cancer tissue samples in Iran

Dowran, Razieh; Joharinia, Negar; Safaei, Akbar; Bakhtiyarizadeh, Sahar; Soleimani, Abootaleb Alidadi; Alizadeh, Rasool; Mir-Shiri, Sara; Sarvari, Jamal

doi:10.1186/s13104-019-4178-3

Cited by 20 publications

(15 citation statements)

References 34 publications

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“…54 However, EBV DNA was not detected in FFPE tissues of Iranian women using PCR targeting the Bam HI-H rightward open reading frame 1 (BHRF1) region. 55 Moreover, EBV DNA was detected using PCR in 20% of fresh tissues of Egyptian BC patients, 56 4.7% of Mexican BC patients, 57 and 31% of Argentinian BC patients. 58 These lower detection rates could be due to differences in storage methods of samples.…”

Section: Discussionmentioning

confidence: 99%

Presence of HPV, EBV and HMTV Viruses Among Egyptian Breast Cancer Women: Molecular Detection and Clinical Relevance

Metwally¹,

Abo-Shadi²,

Fattah³

et al. 2021

IDR

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Background Oncogenic viruses, their possible association with breast cancer (BC) and effect on its clinical course are interesting issue. The present study evaluates the presence of human papillomavirus (HPV), EpsteinBarr virus (EBV), and human mammary tumor virus (HMTV) in BC and their relation with clinico-pathological characteristics. Patients and Methods This study was conducted on 80 Egyptian women with BC and 30 control women without known oncological disease. Forty formalin-fixed paraffin-embedded (FFPE) tissues, forty fresh tissue samples, and white blood cells (WBCs) of BC patients and WBCs of controls were subjected to a qualitative polymerase chain reaction (PCR). Quantitative real-time PCR was used to measure viral loads in fresh tissues of BC. The result was correlated with clinico-pathological characteristics of BC. Results HPV was detected in 33 (41.25%), EBV in 30 (37.5%) and HMTV in 33 (41.25%) BC patients. None of the control women was positive for HPV or EBV while HMTV was detected in 7 (23.3%). Among 40 BC WBCs specimens, HPV/HMTV were found together in 25%, followed by EBV/HMTV in 2.5% and EBV/HPV in 2.5%. However, the three viruses (HPV/EBV/HMTV) were found together in only 5%. In the 40 fresh BC tissues, the three viruses were found together in 12 (30%), EBV/HMTV in 7 (17.5%), HPV/HMTV in 4 (10%), and HPV/EBV in 4 (10%). EBV, HMTV, or multiple viral infections were associated with younger age of BC women. HPV, EBV, and HMTV median loads in fresh tissues were 4.8×10 3 copies/μL, 6.3×10 3 copies/μL, and 97 copies/μL, respectively. Conclusion WBCs could be a more suitable specimen instead of fresh tissue for HMTV detection in BC patients to avoid invasive procedures. The presence of HPV, EBV, and HMTV together in Egyptian women with BC was significantly associated with younger age.

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Section: Discussionmentioning

confidence: 99%

Presence of HPV, EBV and HMTV Viruses Among Egyptian Breast Cancer Women: Molecular Detection and Clinical Relevance

Metwally¹,

Abo-Shadi²,

Fattah³

et al. 2021

IDR

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“…Furthermore, Littman et al reported that EBV antibody titers and old-age at primary infection with EBV might have a role in the etiology of ovarian cancer ( 38 ). Although it is early to conclude the role of EBV in the development of ovarian cancer, the role of this virus in some important human cancers such as, Burkitt lymphoma, nasopharyngeal carcinoma and gastric cancer is well-defined ( 39 ).…”

Section: Discussionmentioning

confidence: 99%

Association of Human Papilloma Virus and Epstein-Barr Virus with Ovarian Cancer in Shiraz, Southwestern Iran

et al. 2020

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Scan to discover online Background & Objective: Ovarian cancer is one of the most common cancers amongst women. The association of Human papillomavirus (HPV) and Epstein-Barr virus (EBV) with ovarian cancer is inconclusive; therefore, the aims of this study were to evaluate the frequency of HPV and EBV in malignant, borderline, benign and normal ovarian tissues. Methods: In this case-control study, 205 Paraffin-embedded ovarian tissue specimens including 68 malignant, 27 borderline, 65 benign, and 45 normal tissues were included from December 2014 to January 2018 and subjected to DNA extraction. The β-globin gene was amplified using PCR to confirm the quality of the extracted DNA. The genomes of HPV (genotypes 16 and 18) and EBV were identified, using specific primers by PCR. Results: The mean age of participants was 43.42 ± 15.4 years. The frequency of HPV was statistically significant between malignant versus benign (P=0.02) and control groups (P=0.002), but not with borderline tumor group (P=0.78). Amongst HPV infected samples, 1 (4.5%) and 14 (63.6%) samples were infected with types 16 and 18, respectively. Also 4 (18.2 %) samples were infected with both genotypes. Eleven samples including 7(10.3%) malignant, 1 (3.7%) borderline, 3 (4.6%) benign and none (0%) of normal control groups were infected with EBV, which was statistically different between malignant and the normal control group (P=0.03). Conclusion: The results of our study showed the possible role of high risk HPVs as well as EBV in pathogenesis of ovarian cancer, and further studies are recommended to confirm these findings.

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“…Biopsies of the uterine cervix showed that the EBV receptors are mostly expressed on ecto-and endo-cervix regions, therefore, it might be involved in the development of cervical cancer as a "helper" factor (Thoe et al, 1993;Shi et al, 2016). Regarding new reports, the presence of EBV has been documented in other cancers including breast, prostate, oral, and salivary gland carcinomas (Sarvari et al, 2018;Dowran et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Co-existing of HSV1/2 or EBV Infection with the Presence of High-Risk HPV DNA in Cervical Lesions in the Southwest of Iran

Joharinia

Faghihinejad

Seyedi

et al. 2020

Asian Pac J Cancer Prev

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Objective: While the vast majority of the cervical lesions have been attributed to the HPVs, the role of EBV and HSV1/2 as co-factors in the progression of these abnormalities needs more investigation. In this study, we aimed to determine the coexistence of EBV or HSV in cervical lesions infected with high-risk HPVs. Methods: Totally, 102 formaline-fixed cervical lesions with different pathological grades (LSIL, HSIL, and SCC) were enrolled in this study. DNA was extracted, and its integrity was examined by PCR assay. Two conventional PCRs were performed for the detection of EBV and HSV1/2 genomes in the tissue specimens. Besides, an in-house Real-Time PCR, as well as a nested PCR assays following sequencing, was performed to detect HPV genotypes in EBV or HSV positive samples. Results: The mean age of the participants was 42.8±13 years. Out of 102 samples, 32% (n=33) were confirmed to be LSIL, 42.2% (n=43) were HSIL, 22.5% (n=23) were SCC and 2.9% (n=3) were adenocarcinoma. EBV genome was detected in 13(12.7%) samples including 2 of LSIL, 8 of HSIL and 3 of SCC. All EBV positive samples harbored high risk HPV types 16,18 and/or 31 co-infections. However, the HSV genome was not found in any of the samples. Conclusion: Our result revealed that the frequency of EBV infection is higher in HISL than LSIL. Moreover, the amount of HPV load showed an elevated level among co-infected patients, which indicates that EBV might be an enhancing factor of disease progression. In contrast, HSV may not has a role as a co-factor in cervical lesions pathogenesis.

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No detection of EBV, BKV and JCV in breast cancer tissue samples in Iran

Cited by 20 publications

References 34 publications

Presence of HPV, EBV and HMTV Viruses Among Egyptian Breast Cancer Women: Molecular Detection and Clinical Relevance

Presence of HPV, EBV and HMTV Viruses Among Egyptian Breast Cancer Women: Molecular Detection and Clinical Relevance

Association of Human Papilloma Virus and Epstein-Barr Virus with Ovarian Cancer in Shiraz, Southwestern Iran

Co-existing of HSV1/2 or EBV Infection with the Presence of High-Risk HPV DNA in Cervical Lesions in the Southwest of Iran

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