No cultural detection of Helicobacter pylori in dental plaque

Recklinghausen, G. von; Weischer, Thomas; Ansorg, R.; Mohr, Christopher

doi:10.1016/s0934-8840(11)80643-2

Cited by 20 publications

(14 citation statements)

References 9 publications

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“…5,21,27 However, investigators have tried to detect H. pylori in the dental plaque samples by the culture method and reported that H. pylori was not cultivated from the samples investigated. 28 The failure of culture methods to detect H. pylori from dental plaque may be due to a variety of reasons, such low numbers of the organism or existence of non-cultivable forms in dental plaque. Other investigators employing polymerase chain reaction for the detection of H. pylori from subgingival plaque samples in periodontitis patients have also failed to reveal the specific amplification-product characteristic of H. pylori, suggesting that periodontal pockets do not constitute a natural reservoir for H. pylori.…”

Section: Sanitary Conditions Of Cases and Controlsmentioning

confidence: 99%

Are Dental Plaque, Poor Oral Hygiene, and Periodontal Disease Associated With Helicobacter pylori Infection?

Anand

Nandakumar

Shenoy

2006

Journal of Periodontology

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Background: The microorganism Helicobacter pylori has been closely linked to chronic gastritis, peptic ulcer, gastric cancer, and mucosa‐associated lymphoid tissue (MALT) lymphoma. Despite the current treatment regimens that lead to successful management of H. pylori‐positive chronic gastritis, the reinfection rate is high. It has been suggested that one of the possible mechanisms of reinfection is the recolonization from dental plaque. The purpose of this study was to determine whether dental plaque, poor oral hygiene, and periodontal disease were risk factors for H. pylori infection. Methods: Among the 134 patients, 65 patients who had a positive H. pylori serology or positive rapid urease test or histologic evidence for the presence of H. pylori in antral biopsy specimens were categorized as cases. The remaining 69 patients who were negative for H. pylori serology, the rapid urease test, and histology were controls. Results: It was found that the association of periodontal disease and poor oral hygiene with H. pylori infection was not significant. There was a higher prevalence of H. pylori in the dental plaque of patients with gastric H. pylori infection than in controls, but both groups had a surprisingly high positive urease test for H. pylori in plaque (89% and 71%, respectively). Conclusions: H. pylori in dental plaque is seldom eliminated by H. pylori‐eradication therapy, and this may act as a source for future reinfection. Hence, eradication of H. pylori from the dental plaque should be made an important part of comprehensive management of H. pylori‐associated gastric diseases.

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Section: Sanitary Conditions Of Cases and Controlsmentioning

confidence: 99%

Are Dental Plaque, Poor Oral Hygiene, and Periodontal Disease Associated With Helicobacter pylori Infection?

Anand

Nandakumar

Shenoy

2006

Journal of Periodontology

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“…3.17.20,25-29,35,42,43 The lack 0f culture growth compared to PCR identification would be due to insufficient numbers of cells for detection, to the presence of inhibitors, or to the presence of unculturable but viable coccoid forms in polymicrobial oral specimens. Some researchers have suggested that dental plaque and saliva cannot be permanent reservoirs for H. pylori and that occasional recovery of viable microorganisms in the mouth is associated with gastroesophageal reflux.719-22 [33][34][35][36][37][38][39][40][41] Nonetheless, there are some arguments against permanent oral colonization.…”

Section: Oral Carriage Of H Pylori and Implicationsmentioning

confidence: 99%

Oral Carriage of Helicobacter pylori: A Review

Madinier

Fosse

Monteil

1997

Journal of Periodontology

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Helicobacter pylori is a microaerophilic, motile bacterium, especially adapted to life in the human stomach. The presence of H. pylori in the stomach is strongly associated with chronic gastritis and ulcer disease and is a risk factor for gastric cancers. The microorganism may be transmitted orally and has been detected in dental plaque, saliva, and feces, but the hypothesis that oral microflora may be a permanent reservoir of H. pylori is still controversial. A review of the literature suggests that the recovery of H. pylori in the mouth is probably intermittent, associated with gastroesophageal reflux but not with specific oral disease. Nonetheless, the PCR identification of oral H. pylori may become helpful, particularly in cases of gastritis or ulcer relapse after antimicrobial therapy. Eradication of oral H. pylori by local medication or periodontal procedures would rely on the precise identification of its ecological niche. Within family groups, prophylactic methods should be practiced to avoid oral carriage of H. pylori. The risk of iatrogenic transmission during dental care, however, is already circumscribed by standard professional hygiene procedures. J Periodontol 1997;68:2–6.

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“…The recovery rate of H. pylori from the oral cavity is controversial; while H. pylori could be isolated from the oral cavity in some cases [9,12,16], most attempts to culture the organism have failed [17,18]. Some investigators [18] have suggested that non-cultivable coccoid forms of the organism may survive in the mouth.…”

Section: Introductionmentioning

confidence: 99%

“…Some investigators [18] have suggested that non-cultivable coccoid forms of the organism may survive in the mouth. Many PCR assays have been developed for detecting H. pylori in the oral cavity [19], mostly based on the urease gene sequences and 16S ribosomal RNA genes.…”

Section: Introductionmentioning

confidence: 99%