No Correlation in Epstein-Barr Virus Reactivation Between Serological Parameters and Viral Load

Гартнер, Барбара; Kortmann, Kerstin; Schäfer, Marco; Mueller‐Lantzsch, Nikolaus; Sester, Urban; Kaul, Harald; Pees, Hanns

doi:10.1128/jcm.38.6.2458-2458.2000

Cited by 40 publications

(19 citation statements)

References 5 publications

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“…The generally low EBV loads in both groups are in agreement with previous reports for immune-competent individuals. Several investigators reported less than 5 Geq Â 10 À5 PBLs during latent infection in healthy persons [Babcock et al, 1999;Kimura et al, 1999;Gartner et al, 2000;Maurmann et al, 2003]. On the other hand, nine athlete samples in the current study had higher viral loads, whereas only three controls surpassed this value.…”

Section: Discussioncontrasting

confidence: 48%

“…Longitudinal testing demonstrated a prolonged seroconversion, with 18.4 Geq Â 10 À5 PBLs and a weak immune response of only equivocal IgG by ELISA and 1þ VCA and EBNA-1 bands in immunoblot. EBVspecific IgG levels did not correlate well with EBV loads confirming previous data [Gartner et al, 2000;Maurmann et al, 2003]. Luderer et al [2005] reported detectable EBV DNA, in as little as 3% of the sera with EA antibodies, illustrating a low sensitivity of EA antibodies for detecting genuine reactivations.…”

Section: Discussionmentioning

confidence: 45%

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Elevated Epstein–Barr virus loads and lower antibody titers in competitive athletes

Hoffmann¹,

Wolfarth²,

Hörterer³

et al. 2010

Journal of Medical Virology

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Epstein-Barr virus (EBV) is a persisting herpesvirus which is controlled by the adaptive immune response after primary infection and maintained in a latent state. However, reactivation or persistent replication is observed in situations where the immune response is compromised. Since intensive physical training has been reported to diminish immune function, increased EBV load may be a cause of reduced performance and decreased ability to sustain high training loads in competitive athletes. Samples drawn from 209 athletes during their regular follow-up appointments were tested. One hundred sixty-five individuals of similar age not active in competitive sports served as case-controls. EBV load was quantified in peripheral blood leucocytes (PBLs) by real-time PCR, and EBV antibodies were detected in plasma by ELISA and immunoblot analysis. EBV DNA was detectable in 25 of 209 athletes and in 26 of 165 controls. Of note, the EBV load per 10(5) PBLs was 6.44 +/- 1.75 in the case and 1.67 +/- 0.44 copies in the controls, yielding a high significant difference (P < 0.0001). However, EBV-specific IgG titers were significantly lower in athletes (150.4 +/- 10.73 U ml(-1) vs. 241.6 +/- 18.59 U ml(-1)). As monitored by immunoblotting, primary infections were detected with low prevalence, three in the case group and one in the control group. These findings demonstrate that EBV is present at higher levels in athletes, but the antibody response is lower in athletes than in the controls. J. Med. Virol. 82:446-451, 2010. (c) 2010 Wiley-Liss, Inc.

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Section: Discussioncontrasting

confidence: 48%

Section: Discussionmentioning

confidence: 45%

Elevated Epstein–Barr virus loads and lower antibody titers in competitive athletes

Hoffmann¹,

Wolfarth²,

Hörterer³

et al. 2010

Journal of Medical Virology

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“…EBV and KSHV DNA are detected infrequently in serum or plasma from healthy individuals, but there is an increased prevalence in cancer patients and in HIV-1-infected individuals [35,36]. A consistent correlation between EBV or KSHV DNA levels in serum or plasma and antibody titers to viral proteins has not been apparent [37]. To see if the more global antibody responses detected on the arrays would provide any additional insight, we examined KSHV and EBV DNA levels in the plasma from HIV-positive KS patients (see Figure 2A).…”

Section: Comparison Of Virus Load In Plasma Versus Humoral Immune Resmentioning

confidence: 99%

Comparison of Humoral Immune Responses to Epstein-Barr Virus and Kaposi’s Sarcoma–Associated Herpesvirus Using a Viral Proteome Microarray

Zheng

Wan²,

Cho

et al. 2011

The Journal of Infectious Diseases

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“…Diagnosis of LRTI in HCT recipients is particularly challenging due to high rates of non-infectious inflammatory conditions such as graft versus host disease (GVHD) that can drive pulmonary inflammation, induce fever and mimic infection (7)(8)(9)(10). Furthermore, the diagnostic yield of traditional tests is reduced in HCT patients due to antimicrobial prophylaxis, reduced antibody titers, and infections from uncommon opportunistic microorganisms (11,12).…”

Section: Introductionmentioning

confidence: 99%

Metagenomic Sequencing Detects Respiratory Pathogens in Hematopoietic Cellular Transplant Patients

Langelier

Zinter

Kalantar

et al. 2017

Preprint

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Commentary: Lower respiratory tract infections are a leading cause of death in hematopoietic cellular transplant recipients, but the microbiologic etiology of these infections is frequently unknown. The limits of current diagnostics preclude targeted antimicrobial treatments, resulting in excess morbidity and mortality. Metagenomic next-generation sequencing may enable precision diagnosis of respiratory infections in hematopoietic cellular transplant patients by simultaneously detecting microbial pathogens, transcriptional biomarkers of the host response, and the pulmonary microbiome. This approach may facilitate targeted antimicrobial prescription and provide a new method for distinguishing infectious and non-infectious post-transplant respiratory illness. ABSTRACT RATIONALE: Current microbiologic diagnostics often fail to identify the etiology of lower respiratory tract infections (LRTI) in hematopoietic cellular transplant recipients (HCT), which precludes the implementation of targeted therapies. OBJECTIVES: To address the need for improved LRTI diagnostics, we evaluated the utility of metagenomic next generation sequencing (mNGS) of bronchoalveolar lavage (BAL) to detect microbial pathogens in HCT patients with acute respiratory illnesses. METHODS: We enrolled 22 post-HCT adults ages 19-69 years with acute respiratory illnesses who underwent BAL at the University of Michigan between January 2012 and May 2013. mNGS was performed on BAL fluid to detect microbes and simultaneously assess the host transcriptional response. Results were compared against conventional microbiologic assays. MEASUREMENTS & MAIN RESULTS: mNGS demonstrated 100% sensitivity for detecting respiratory microbes (human metapneumovirus, respiratory syncytial virus, Stenotrophomonas maltophilia, human herpesvirus 6 and cytomegalovirus) when compared to standard testing.Previously unrecognized LRTI pathogens were identified in six patients for whom standard testing was negative (human coronavirus 229E, human rhinovirus A, Corynebacterium propinquum and Streptococcus mitis); findings were confirmed by independent PCR and 16S rRNA sequencing. Relative to patients without infection, patients with infection had increased expression of immunity related genes (p=0.022) and significantly lower diversity of their respiratory microbiome (p=0.017). CONCLUSIONS:Compared to conventional diagnostics, mNGS enhanced detection of pathogens in BAL fluid from HCT patients. Furthermore, our results suggest that combining unbiased microbial pathogen detection with assessment of host gene biomarkers of immune response may hold promise for enhancing the diagnosis of post-HCT respiratory infections. Abstract Word Count: 249

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No Correlation in Epstein-Barr Virus Reactivation Between Serological Parameters and Viral Load

Cited by 40 publications

References 5 publications

Elevated Epstein–Barr virus loads and lower antibody titers in competitive athletes

Elevated Epstein–Barr virus loads and lower antibody titers in competitive athletes

Comparison of Humoral Immune Responses to Epstein-Barr Virus and Kaposi’s Sarcoma–Associated Herpesvirus Using a Viral Proteome Microarray

Metagenomic Sequencing Detects Respiratory Pathogens in Hematopoietic Cellular Transplant Patients

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