No association of brain‐derived neurotrophic factor Val66Met polymorphism with anorexia nervosa in Japanese

Abstract

The Met66 allele of the Val66Met polymorphism in the brain-derived neurotrophic factor (BDNF) gene has been reported to be associated with anorexia nervosa (AN), and also lower minimum body mass index (BMI) and higher harm avoidance in AN. We genotyped the Val66Met polymorphism (rs6265) in 689 AN cases and 573 control subjects. There were no significant differences in the genotype or allele frequencies of the Val66Met between AN and control subjects (allele wise, odds ratio = 0.920, 95% CI 0.785-1.079, P = 0.305). No difference was found in minimum BMIs related to Val66Met in AN (one-way ANOVA, P > 0.05). Harm avoidance scores on the Temperament and Character Inventory were lower in the Met66 allele carriers (P = 0.0074) contrary to the previous report. Thus we were unable to replicate the previous findings that the Met66 allele of the BDNF is associated with AN and that the minimum BMI is lower or the harm avoidance score is higher in AN patients with the Met66 allele.

Discussing the Putative Role of Obesity-Associated Genes in the Etiopathogenesis of Eating Disorders

“…Rybakowski et al could not find an association between the Val66Met and temperamental traits in AN [106], which is consistent with the findings reported recently by our group in AN and BN using a different questionnaire, the Eating Disorder Inventory -2 (EDI-2) [107]. In contrast, Ando et al did observe significant lower harm avoidance scores in AN patients who were Met66 carriers, although there were no differences for novelty seeking, reward dependence or persistence [108]. With regard to the C-270T SNP, the T allele has been shown to correlate with higher persistence and harm avoidance scores in AN patients [106].…”

The tyrosine kinase receptor Tyro3 enhances lifespan and neuropeptide Y (Npy) neuron survival in the mouseanorexia(anx) mutation

“…With respect to neurotrophic factors and tyrosine kinase receptor signaling, candidate gene approaches have obtained mixed results for linkage of the rs6265 (V66M) polymorphism in brain-derived neurotrophic factor ( BDNF ) with ANR (Dardennes et al, 2007; Ribasés et al, 2005, 2004; Ando et al, 2012; Brandys et al, 2013; Rybakowski et al, 2007). In the case of appetite-regulating neuropeptides, so far missense mutations in POMC have been reported in one individual with ANR, but no associations with common variants have been detected in ANR populations (Yilmaz et al, 2014).…”

Identificação de polimorfismos de genes candidatos nos transtornos alimentares

“…Assim, a variabilidade e o papel do gene do BDNF nos TAs em futuras pesquisas poderão elucidar o que essas variáveis podem causar e revelar se esse gene é um marcador biológico da doença, além das interações gene/ambiente e gene/gene que provavelmente dificultam esses achados. [32][33][34][35] A análise do SNP do gene ERb no presente estudo demonstrou que os resultados não apresentaram diferenças significativas entre os grupos estudados para o SNP 1730A®G, tanto no genótipo AA (pX 2 =0,086) quanto no genótipo GG (pX 2 =0,064). Esses achados corroboram outros dados da literatura.…”