No association between polymorphisms/haplotypes of the vascular endothelial growth factor gene and preeclampsia

Abstract

BackgroundPreeclampsia (PE) is the first worldwide cause of death in pregnant women, intra-uterine growth retardation, and fetal prematurity. Some vascular endothelial grown factor gene (VEGF) polymorphisms have been associated to PE and other pregnancy disturbances. We evaluated the associations between VEGF genotypes/haplotypes and PE in Mexican women.Methods164 pregnant women were enrolled in a case-control study (78 cases and 86 normotensive pregnant controls). The rs699947 (-2578C/A), rs1570360 (-1154G/A), rs2010963 (+405G/C), and rs25648 (-7C/T), VEGF variants were discriminated using Polymerase Chain Reaction - Restriction Fragment Length Polymorphism (PCR-RFLP) methods or Taqman single nucleotide polymorphism (SNP) assays.ResultsThe proportions of the minor allele for rs699947, rs1570360, rs2010963, and rs25648 VEGF SNPs were 0.33, 0.2, 0.39, and 0.17 in controls, and 0.39, 0.23, 0.41, and 0.15 in cases, respectively (P values > 0.05). The most frequent haplotypes of rs699947, rs1570360, rs2010963, and rs25648 VEGF SNPs, were C-G-C-C and C-G-G-C with frequencies of 0.39, 0.21 in cases and 0.37, 0.25 in controls, respectively (P values > 0.05)ConclusionThere was no evidence of an association between VEGF alleles, genotypes, or haplotypes frequencies and PE in our study.

Positive association between vascular endothelial growth factor (VEGF) -2578 C/A variant and prostate cancer

“…We report that this SNP may modify the risk of PCa up to 84%, supporting its involvement in prostate carcinogenesis. Despite that in Mexico there are no studies of -2578 C/A polymorphism and PCa, the frequency of -2578 A/A genotype has been reported in a range of 11-12% in Northwestern Mexican women [13] and Mexican ancestry participants of the HapMap International Project, respectively [29]. These data suggest that the low frequency of A/A carriers in the cases group observed in our study was directly related with their status of disease.…”

“…The -2578 C/A polymorphism of VEGF gene was selected considering some premises: 1) the high prevalence of aggressive tumors reported in a previous Mexican early PCa screening PSA and DRE-based [16], 2) tumor aggressiveness is related with angiogenesis, while a high VEGF expression in cancer is considered as an unfavorable prognosis marker, 3) in addition to their association with cancer in other populations, -2578 C/A VEGF polymorphism has an impact in the mRNA level, and finally, 4) -2578 VEGF A/A genotype frequency was reported in a 10% among Northwestern Mexican population [13]; therefore, their potential association with PCa could have an impact in an important proportion of Mexican men.…”

“…The genotype C/C of the SNP rs699947 in the -2578 nucleotide position of the VEGF promoter, has been associated with higher levels of VEGF in blood mononuclear cells [12]. This polymorphism correlates with disease stages in which angiogenesis plays a critical role [9,[13][14][15]. Despite the high prevalence of aggressive prostate tumors in Mexico [16], there are no association reports between -2578 C/A VEGF polymorphism and risk of PCa.…”

“…The allelic discrimination of -2578 C/A VEGF variant (rs699947) was performed by polymerase chain reaction coupled to restriction fragments of length polymorphisms (PCR-RFLP) according to protocol, primer, reagents, and cycling conditions, reported by Garza-Veloz et al [13]. The PCR-RFLP products (C/C: 285 bp; C/A: 285 bp, 206 bp, 79 bp; A/A: 206 bp, 79 bp, respectively) were electrophoresed through 3.0% agarose gels, and visualized by ethidium bromide staining.…”

A legjelentősebb angiogeneticus gének méhlepényi expressziójának változásai a méhen belüli növekedési visszamaradás hátterében

“…Noha az érképződési zavar teljes mechanizmusának biológiai háttere egyelőre felderítetlen, az angiogeneticus faktorok működészavarát több szerző is erősen valószínűsíti [12,[23][24][25]. Míg állatkísérletekben első-sorban a VEGF-A csökkent működése eredményezte IUGR kialakulását, addig a humán mintán végzett vizsgálatok elsősorban a PlGF érintettségére utaló eredmé-nyeket mutattak [26][27][28][29][30].…”