No association between Epstein-Barr Virus and Mouse Mammary Tumor Virus with Breast Cancer in Mexican Women

Morales-Sánchez, Abigail; Molina-Muñoz, Tzindilú; Martínez-López, Juan L. E.; Hernández-Sancén, Paulina; Mantilla, Alejandra; Leal, Yelda A.; Torres, Javier; Fuentes‐Pananá, Ezequiel M.

doi:10.1038/srep02970

Cited by 37 publications

(32 citation statements)

References 31 publications

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“…Therefore, although in silico preliminary analyses can help to predict the scope of experimental strategies, we recommend considering this difference in future studies. Notably, we found no reads for MMTV and HPV and we confirmed the absence of MMTV in BrC by PCR ( Morales-Sánchez et al 2013 ). In this study, we developed and in silico pipeline of analysis of viral sequences in tumour samples, which was then used to sequence BrC and GC tumours samples, finding evidence of EBV in GC, a result that validated the sequencing and analysis strategy.…”

Section: Discussionsupporting

confidence: 67%

Assessment of Epstein-Barr virus nucleic acids in gastric but not in breast cancer by next-generation sequencing of pooled Mexican samples

Fuentes‐Pananá

Larios-Serrato²,

Méndez‐Tenorio³

et al. 2016

Mem. Inst. Oswaldo Cruz

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Gastric (GC) and breast (BrC) cancer are two of the most common and deadly tumours. Different lines of evidence suggest a possible causative role of viral infections for both GC and BrC. Wide genome sequencing (WGS) technologies allow searching for viral agents in tissues of patients with cancer. These technologies have already contributed to establish virus-cancer associations as well as to discovery new tumour viruses. The objective of this study was to document possible associations of viral infection with GC and BrC in Mexican patients. In order to gain idea about cost effective conditions of experimental sequencing, we first carried out an in silico simulation of WGS. The next-generation-platform IlluminaGallx was then used to sequence GC and BrC tumour samples. While we did not find viral sequences in tissues from BrC patients, multiple reads matching Epstein-Barr virus (EBV) sequences were found in GC tissues. An end-point polymerase chain reaction confirmed an enrichment of EBV sequences in one of the GC samples sequenced, validating the next-generation sequencing-bioinformatics pipeline.

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Section: Discussionsupporting

confidence: 67%

Assessment of Epstein-Barr virus nucleic acids in gastric but not in breast cancer by next-generation sequencing of pooled Mexican samples

Fuentes‐Pananá

Larios-Serrato²,

Méndez‐Tenorio³

et al. 2016

Mem. Inst. Oswaldo Cruz

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“…We determined that using 15.5 × 10 3 cells from samples (and 15.5 × 10 3 plasmid copies mixed with DNA from 15.5 × 10 3 negative cells as control) would be sufficient to detect up to one viral genome per cell even in samples with only 25% of leukemic blasts (Figures 1(b) , 1(c) , and 1(d) ). We have previously used these PCRs in which the frequency of infected cells matches the frequency of cancer cells to address direct viral oncogenesis [ 28 – 30 ].…”

Section: Resultsmentioning

confidence: 99%

EBV, HCMV, HHV6, and HHV7 Screening in Bone Marrow Samples from Children with Acute Lymphoblastic Leukemia

Morales-Sánchez

Pompa-Mera

Fajardo-Gutiérrez

et al. 2014

BioMed Research International

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Acute lymphoblastic leukemia (ALL) is the most common cancer in childhood worldwide and Mexico has reported one of the highest incidence rates. An infectious etiology has been suggested and supported by epidemiological evidences; however, the identity of the involved agent(s) is not known. We considered that early transmitted lymphotropic herpes viruses were good candidates, since transforming mechanisms have been described for them and some are already associated with human cancers. In this study we interrogated the direct role of EBV, HCMV, HHV6, and HHV7 human herpes viruses in childhood ALL. Viral genomes were screened in 70 bone marrow samples from ALL patients through standard and a more sensitive nested PCR. Positive samples were detected only by nested PCR indicating a low level of infection. Our result argues that viral genomes were not present in all leukemic cells, and, hence, infection most likely was not part of the initial genetic lesions leading to ALL. The high statistical power of the study suggested that these agents are not involved in the genesis of ALL in Mexican children. Additional analysis showed that detected infections or coinfections were not associated with prognosis.

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“…2016; 11(3):e34806. (11,18,(36)(37)(38)(39)(40)(41)(42)(43)(44)(45)(46)(47)(48)(49)(50)(51)(52)(53). Table 1 shows a summary of the EBV-DNA positive subjects according to nationality, types of sample, and kinds of control tissue in the 20 selected studies.…”

Section: Resultsmentioning

confidence: 99%

Epstein-Barr Virus Infection and Risk of Breast Cancer: An Adaptive Meta-Analysis for Case-Control Studies

Bae

Kim

2016

Arch Clin Infect Dis

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Context: The association between 'Epstein-Barr' virus (EBV) and breast cancer risk still remains controversial. A Systemic Review (SR) published in 2012 reported that there might be a statistically significant association between EBV and risk of breast cancer. However, errors were found in the appraisal process of the concerned SR. Objectives:The aim of this report was to conduct an adaptive meta-analysis with additional extraction of relevant papers published up until September 2015.Data Sources: The lists of references, cited articles and related articles provided by PubMed were made using the articles selected in the previous SR.Study Selection: Among these articles, only case-control studies using Polymerase Chain Reaction (PCR) techniques to detect EBV DNA in tissues were selected. Data Extraction: The summary odds ratio (SOR) and the 95% confidence interval (CI) were calculated though meta-analysis.Results: A total of 20 case-control studies were selected, and the total numbers of subjects in the case and control groups were 1947 and 1010, respectively. The findings of this meta-analysis revealed that EBV infection might increase the risk of breast cancer (SOR = 3.84, 95% CI: 2.24-6.58; I-squared = 62.2%). Sub-group analyses by region, sample type, and control tissue showed that a statistical significance of the risk was still secured. Conclusions:The findings of the meta-epidemiological study support that EBV infection may increase the risk of breast cancer. Nested case-control studies are required in the future.

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No association between Epstein-Barr Virus and Mouse Mammary Tumor Virus with Breast Cancer in Mexican Women

Cited by 37 publications

References 31 publications

Assessment of Epstein-Barr virus nucleic acids in gastric but not in breast cancer by next-generation sequencing of pooled Mexican samples

Assessment of Epstein-Barr virus nucleic acids in gastric but not in breast cancer by next-generation sequencing of pooled Mexican samples

EBV, HCMV, HHV6, and HHV7 Screening in Bone Marrow Samples from Children with Acute Lymphoblastic Leukemia

Epstein-Barr Virus Infection and Risk of Breast Cancer: An Adaptive Meta-Analysis for Case-Control Studies

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