NO-1886 suppresses diet-induced insulin resistance and cholesterol accumulation through STAT5-dependent upregulation of IGF1 and CYP7A1

Li, Qinkai; Yin, Wang; Cai, Manbo; Liu, Yi; Hou, Hongjie; Shen, Qingyun; Zhang, Chi; Xiao, Jun-Xia; Wu, Qishisan; Funaki, Makoto; Nakaya, Yutaka

doi:10.1677/joe-09-0278

Cited by 14 publications

(21 citation statements)

References 55 publications

(53 reference statements)

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“…Western blot analyses were performed as described previously [15]. Briefly, after treating the cells as described in the figure legends, cells were rinsed three times with ice‐cold PBS and lysed in RIPA buffer.…”

Section: Methodsmentioning

confidence: 99%

The density of extracellular matrix proteins regulates inflammation and insulin signaling in adipocytes

Hata

Kosugi

et al. 2010

FEBS Letters

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a b s t r a c tCells can not only sense the type of extracellular matrix (ECM) protein that is present in the microenvironment, but they can also sense its density. Here, we investigated the effects of ECM protein density on adipokine secretion and insulin signaling in adipocytes. To this end, 3T3-L1 adipocytes were cultured on the surface of polyacrylamide gels that were coated with gradient densities of a collagen type I and fibronectin mixture. We found that high density ECM causes a decrease in insulin signaling and adiponectin secretion, whereas the secretion of monocyte chemoattractant protein-1 (MCP-1) was increased via the activation of nuclear factor-jB (NF-jB). These results indicate that the density of the ECM directly regulates the inflammatory response and insulin sensitivity of adipocytes. Structured summary:MINT-7992217: Irs1 (uniprotkb:P35569) physically interacts (MI:0915) with phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha (uniprotkb:P26450) by anti bait coimmunoprecipitation (MI:0006)

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Section: Methodsmentioning

confidence: 99%

The density of extracellular matrix proteins regulates inflammation and insulin signaling in adipocytes

Hata

Kosugi

et al. 2010

FEBS Letters

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“…Serum cortisol and IGF-I concentrations were determined by RIA, according to the manufacturer's instructions (NT Go. Ltd., Tianjin, Ghina), using commercial RIA kits (Li et al, 2010).…”

Section: Growth Performance Blood Biochemical Indices and Hormone Cmentioning

confidence: 99%

Sorbic acid improves growth performance and regulates insulin-like growth factor system gene expression in swine1

Luo¹,

Fang²,

Shu³

et al. 2011

Journal of Animal Science

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Sorbic acid (SA) is a PUFA with a conjugated double bond. The conjugated fatty acids, including CLA, are multifunctional bioactive fatty acids with the ability to improve growth performance. The effect of SA on pig growth performance was examined to determine its mechanism of action. The ADG, ADFI, and serum IGF-I concentration were examined, as were IGF-I secretion and IGF system gene expression in hepatocytes. Two hundred forty 21-d-old Duroc × Landrace × Yorkshire weaned piglets (6.86 ± 0.02 kg) were randomly divided into 4 groups, each consisting of 3 pens of 20 piglets (10 female and 10 male). The 4 groups of piglets were kept in a temperature-controlled room (26 to 28 °C), and feed and water were provided to the pigs ad libitum. Weanling piglets were fed diets that included 0, 0.5, 2, or 4 g of SA/kg for 42 d. The diet supplemented with 0.5 g/kg of SA improved (P < 0.05) ADG, BW, and G:F, whereas supplementation with all 3 SA doses increased (P < 0.05) ADG and G:F at 21 to 42 d of age. The greatest concentration of plasma triglycerides was observed (P < 0.05) in the 4 g/kg of SA group. The SA increased (0.5 g of SA/kg, P > 0.05; 1 g of SA/kg, P < 0.05; and 2 g of SA/kg, P < 0.05, respectively) plasma total serum protein and globulin concentrations in a dose-dependent manner. It was noted that the smallest SA treatment dose (0.5 g/kg) dramatically increased (P < 0.05) serum IGF-I concentration but decreased (P < 0.05) the concentrations of blood urea N and cortisol. The SA increased (P < 0.05) IGF-I, IGF-II, IGF-I receptor (IGF-IR), and PPARα gene mRNA expression and IGF-I secretion, but not (P > 0.05) IGFBP or PPARγ mRNA expression, in pig primary hepatocytes. These results indicate that SA improves growth performance by regulating IGF system gene expression and hormone secretion.

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“…Bama miniature pigs ( sus scrofa domesticus ) are genetically stable, highly inbred, small, and easier to handle than domestic pigs [ 33 ]. They are relatively susceptible to HSHF-diet-induced diabetes [ 4 ], and these HSHF-diet-induced Bama minipigs have been used for IR and diabetes research in the past [ 18 , 19 , 23 ]. The aim of the present study was to determine the effects of an HSHF diet on the impairment of skeletal muscle insulin sensitivity and glucose intolerance in Bama miniature pigs, and then to elucidate the mechanisms involved.…”

Section: Introductionmentioning

confidence: 99%

A high sucrose and high fat diet induced the development of insulin resistance in the skeletal muscle of Bama miniature pigs through the Akt/GLUT4 pathway

et al. 2017

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A high sucrose and high fat (HSHF) diet induces insulin resistance (IR) and increased susceptibility to type 2 diabetes mellitus (T2DM), but the underlying mechanisms are poorly characterized. This study aimed to investigate the molecular mechanisms by which the HSHF diet impairs insulin sensitivity in Bama miniature pigs (sus scrofa domesticus). Twelve Bama miniature pigs were randomly assigned to the control diet (CD) group (n=6) or the HSHF group (n=6) for 6 months. Biochemical parameters were measured. Western blot, RT-qPCR and immunohistochemistry were used to profile the changes of protein expression, mRNA expression and glucose transporter 4 (GLUT4) expression in skeletal muscle tissues, respectively. In comparison to the CD group, the homeostasis model assessment-insulin resistance (HOMA-IR) index of the HSHF group demonstrated a 2.9-fold increase, and the insulin sensitivity showed a 24.8% decrease. Compared with the CD group, p-Akt S473 decreased by approximately 59% and GLUT4 decreased by 43.8% in the skeletal muscle of the HSHF group. However, the expression of p-mTOR S2448 between the 2 groups was not significantly different (P=0.309). This study demonstrates that a 6-month HSHF diet caused IR, decreased insulin sensitivity, and reduced the expression of p-Akt S473 and GLUT4 in the skeletal muscle of Bama miniature pigs.

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NO-1886 suppresses diet-induced insulin resistance and cholesterol accumulation through STAT5-dependent upregulation of IGF1 and CYP7A1

Cited by 14 publications

References 55 publications

The density of extracellular matrix proteins regulates inflammation and insulin signaling in adipocytes

The density of extracellular matrix proteins regulates inflammation and insulin signaling in adipocytes

Sorbic acid improves growth performance and regulates insulin-like growth factor system gene expression in swine1

A high sucrose and high fat diet induced the development of insulin resistance in the skeletal muscle of Bama miniature pigs through the Akt/GLUT4 pathway

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