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Sood, Anil K.; Ahuja, Paramvir Singh; Sharma, Madhu; Sharma, O. P.; Godbole, S

doi:10.1023/a:1016582732531

Cited by 34 publications

(7 citation statements)

References 12 publications

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“…Substantial numbers of in vitro-propagated plants do not survive after transfer from controlled in vitro conditions to ex vitro environment of the green house and later in the open field. Our result demonstrated more than 70% plant survival in the field and is comparable with the earlier report (Sood et al 2002a).…”

Section: Resultssupporting

confidence: 92%

“…Ex Munro, a multipurpose, fast-growing species with strong culms and used in this region of Central Himalaya for various abovementioned purposes including its leaves for good quality fodder for cattle during winter months (Negi et al 1980), holds considerable promise. The use of tissue-culture technique for multiplication of D. hamiltonii has been reported earlier (Chambers et al 1991;Bag 2001;Godbole et al 2002;Sood et al1992Sood et al , 2002a. Although multiple shoot formation has been reported, rooting has been inconsistent with up to 30% rooting (Sood et al 2002a, b).…”

Section: Introductionmentioning

confidence: 93%

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Improved in vitro shoot multiplication and rooting of Dendrocalamus hamiltonii Nees et Arn. Ex Munro: production of genetically uniform plants and field evaluation

2009

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An efficient in vitro regeneration protocol and field performance of a multipurpose bamboo species Dendrocalamus hamiltonii Nees et Arn. Ex Munro has been demonstrated using single node cuttings taken from the lateral branches of a 20-year-old bush. Axillary buds on the nodal explant sprouted within 10 days of culture on Murashige and Skoog (MS) medium without any plant growth substance. High-frequency proliferation was induced on the propagules (small clusters with 3-5 multiple shoots and rhizomatous portions). Subsequent removal of the shoots (about 1.5 cm) from the rhizomatous portion of propagules (shoot cut) influenced the plantlet formation capacity. A multiplication of about 20-folds was achieved on MS medium supplemented with 8 lM BAP and 1 lM NAA. Rooting efficiency was also markedly enhanced ([90%) when the propagules, following shoot cut, were placed on to MS medium supplemented with 100 lM IBA for 10 days and then transferred to IBA-free medium. This is the first report from this species where 20-fold increment in multiplication was observed at the end of second subculture followed by [90% rooting. The hardened plants, established in the field, exhibited normal growth; their physiological performance has been monitored at 6-month intervals. The rate of photosynthesis increased from 3.55 lmol CO 2 m -2 s -1 (hardened, ready for field transfer) to 5.44 lmol m -2 s -1 (6 months of field transfer); following a year of plantation net photosynthesis recorded was 14.0 lmol CO 2 m -2 s -1 while after 1.5 years it was 12.76 lmol CO 2 m -2 s -1 . These values were compared with those observed for the mother bush. Genetic fidelity of these regenerants was established by RAPD analysis advocating clonal propagation of this species through nodal segment culture and its commercial cultivation.

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Section: Resultssupporting

confidence: 92%

Section: Introductionmentioning

confidence: 93%

Improved in vitro shoot multiplication and rooting of Dendrocalamus hamiltonii Nees et Arn. Ex Munro: production of genetically uniform plants and field evaluation

2009

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“…Among them, mercuric chloride (HgCl 2 ) proved to be the best sterilant for nodal explants, while sodium hypochlorite (NaOCl) is preferred by some other authors [119]. It is suggested that 0.1% HgCl 2 was the most efficient for nodal bamboo disinfection, since a high concentration of HgCl 2 could minimize the plant's growth due to the chemical's impact on the internal tissues [125], while the surface sterilization of seeds is usually accomplished by treating them with 0.05-0.1% HgCl 2 for 15-20 min [126]. According to reference [127], when 0.2% HgCl 2 is used to treat B. wamin, it reduces the plant's bud breaking percentage, while sterilizing the D. asper nodal segments with 0.1% HgCl 2 increased the chance of an aseptic culture [16,19,91,93].…”

Section: Explant Surface Sterilizationmentioning

confidence: 99%

A Concise Review of Dendrocalamus asper and Related Bamboos: Germplasm Conservation, Propagation and Molecular Biology

Mustafa

Derise

Yong

et al. 2021

Plants

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Bamboos represent an emerging forest resource of economic significance and provide an avenue for sustainable development of forest resources. The development of the commercial bamboo industry is founded upon efficient molecular and technical approaches for the selection and rapid multiplication of elite germplasm for its subsequent propagation via commercial agro-forestry business enterprises. This review will delve into the micropropagation of Dendrocalamus asper, one of the most widely cultivated commercial varieties of bamboo, and will encompass the selection of germplasm, establishment of explants in vitro and micropropagation techniques. The currently available information pertaining to molecular biology, DNA barcoding and breeding, has been included, and potential areas for future research in the area of genetic engineering and gene regulation have been highlighted. This information will be of relevance to both commercial breeders and molecular biologists who have an interest in establishing bamboo as a crop of the future.

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“…for various species of bamboo micropropagation [29-,31, 33, 35, 36, 38, 39, 65-71]. So it is suggested that 0.1% Mercuric Chloride was more effective for the disinfection of the explants because the high concentration of the HgCl2 retarded the growth of plants due to the impact of chemical in the internal tissues [72]. Wei et al (2015) have reported that the treatment of explants on 0.1% HgCl2 at lower duration enhanced the survival rate of explants and frequency of bud break.…”

Section: Surface Sterilizationmentioning

confidence: 99%

“…Mostly, 0.8% agar was widely used as a gelling agent to solidified/semi-solidified the media which influenced the plant metabolism [32][33][34][35][36][37][38][39]76]. Some researchers also used phytagel (0.2%) or Gelrite (0.2-0.35%) to agar which influenced high bud breaking in B. wamin [72] and shoot proliferation in B. oldhamii [77]. Similarly, it was noted that dwarf and a lower number of shoots per explant in MS solid media due to leaching and browning problems [79].…”

Section: Culture Mediamentioning

confidence: 99%

Regeneration Technique of Bamboo Species through Nodal Segments: A Review

2020

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Micropropagation is an alternative technique to propagate at large scale plants to meet global plant demand. Various researchers have worked on the micropropagation technique to regenerate bamboo species by using nodal segments from years. Contamination, browning, necrosis, and acclimatization with physiological stress are the extreme problems of the micropropagation technique. But, many numbers of papers have been published on micropropagation of the bamboo species through nodal segments as explants. The proliferation of the bamboo shoots is dependent on the season of collection, size of explants, the position of explants, diversity of plants, concentration and combination of plant growth regulators, most adequate culture medium, environmental condition of the equipment, handling, and individual species. Bamboo is a monocarpic fast-growing, tall perennial grass and having the high potential to generate economic and social benefits. It helps to maintain land patterns and control soil erosion. The long life cycle of the bamboo produces a huge amount of seeds but unfortunately, mostly, they are non-viable. So, bamboos are propagated from vegetative by cutting and air layering. However, these methods are only for a small scale and they also tend to destroy large mother plant stocks and difficult to be transported. So, the in vitro propagation technique is useful to obtain large progenies from desired genotypes. Mostly, BAP and TDZ growth hormones are widely used for shoot multiplication and IBA, NAA and IAA are used for root initiation as per developed protocols in tissue culture for large scale production. This review intends to explore an overview of the recent literature reports to summarize the importance of micropropagation by using nodal segments of bamboo species and factors influencing it.

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Untitled

Cited by 34 publications

References 12 publications

Improved in vitro shoot multiplication and rooting of Dendrocalamus hamiltonii Nees et Arn. Ex Munro: production of genetically uniform plants and field evaluation

Improved in vitro shoot multiplication and rooting of Dendrocalamus hamiltonii Nees et Arn. Ex Munro: production of genetically uniform plants and field evaluation

A Concise Review of Dendrocalamus asper and Related Bamboos: Germplasm Conservation, Propagation and Molecular Biology

Regeneration Technique of Bamboo Species through Nodal Segments: A Review

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