nNOS/GSNOR interaction contributes to skeletal muscle differentiation and homeostasis

Montagna, Costanza; Rizza, Salvatore; Cirotti, Claudia; Maiani, Emiliano; Muscaritoli, Maurizio; Musarò, Antonio; Carrı̀, Maria Teresa; Ferraro, Elisabetta; Cecconi, Francesco; Filomeni, Giuseppe

doi:10.1038/s41419-019-1584-3

Cited by 12 publications

(6 citation statements)

References 40 publications

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“…The decrement of ADH5 confirmed our results and indicated that increased levels of nitrosated proteins both in cysteine and tyrosine (although in this case not statistically significant) in A can be due to the inhibition of ADH5/GSNOR denitrosylase activity, with C subjects appearing to be more protected from this negative signaling, sustaining the assumption that being able to denitrosylate well leads to living longer [12,13,26]. Interestingly, the level of the Trx/TrxR system was decreased both in A and C in comparison to Y, suggesting that denitrosylation in aging requires a tight regulation of the ADH5/GSNOR system while the general mechanism protecting cells from oxidative and nitrosative stress and regulating the dithiol/disulfide balance are not involved in the prolonged healthy aging status typical of centenarians.…”

Section: Discussionsupporting

confidence: 85%

“…In cardiac tissue, nitrosation of the ryanodine receptor 2 (RYR2) regulates peripheral vascular tone and β-adrenergic agonist-stimulated cardiac contractility [11]. It has also been demonstrated that the denitrosylase alcohol dehydrogenase 5/S-nitrosoglutathione reductase (alcohol dehydrogenase 5/S-nitrosoglutathione reductase (ADH5/GSNOR)) is downregulated in cells and animal models of aging and that mRNA levels from circulating peripheral blood mononuclear cells (PBMC) are retained in centenarians [12,13]. We expect that nitrosation of proteins could be a more general event and we assume that markers of nitrosative stress can be found not only in cardiac and skeletal muscle tissues but also in bodily fluids.…”

Section: Introductionmentioning

confidence: 99%

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Can Serum Nitrosoproteome Predict Longevity of Aged Women?

Capitanio

Barbacini

Arosio

et al. 2020

IJMS

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Aging is characterized by increase in reactive oxygen (ROS) and nitrogen (RNS) species, key factors of cardiac failure and disuse-induced muscle atrophy. This study focused on serum nitroproteome as a trait of longevity by adopting two complementary gel-based techniques: two-dimensional differential in gel electrophoresis (2-D DIGE) and Nitro-DIGE coupled with mass spectrometry of albumin-depleted serum of aged (A, n = 15) and centenarian (C, n = 15) versus young females (Y, n = 15). Results indicate spots differently expressed in A and C compared to Y and spots changed in A vs. C. Nitro-DIGE revealed nitrosated protein spots in A and C compared to Y and spots changed in A vs. C only (p-value < 0.01). Nitro-proteoforms of alpha-1-antitripsin (SERPINA1), alpha-1-antichimotripsin (SERPINA3), ceruloplasmin (CP), 13 proteoforms of haptoglobin (HP), and inactive glycosyltransferase 25 family member 3 (CERCAM) increased in A vs. Y and C. Conversely, nitrosation levels decreased in C vs. Y and A, for immunoglobulin light chain 1 (IGLC1), serotransferrin (TF), transthyretin (TTR), and vitamin D-binding protein (VDBP). Immunoblottings of alcohol dehydrogenase 5/S-nitrosoglutathione reductase (ADH5/GSNOR) and thioredoxin reductase 1 (TRXR1) indicated lower levels of ADH5 in A vs. Y and C, whereas TRXR1 decreased in A and C in comparison to Y. In conclusion, the study identified putative markers in C of healthy aging and high levels of ADH5/GSNOR that can sustain the denitrosylase activity, promoting longevity.

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Section: Discussionsupporting

confidence: 85%

Section: Introductionmentioning

confidence: 99%

Can Serum Nitrosoproteome Predict Longevity of Aged Women?

Capitanio

Barbacini

Arosio

et al. 2020

IJMS

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“…On cervical dislocation, Achilles tendons of five 8-weeks-old mice were dissected, embedded in Tissue-Tek O.C.T. (4583, Sakura), flash-frozen in liquid nitrogen-cooled isopentane (TCM0167, VWR), and stored at −80 °C until analysis, as previously described [ 41 ]. The longitudinal sections (8 μm thick) were obtained with a Thermo Fisher cryostat (−20 °C) and used for immunostaining.…”

Section: Methodsmentioning

confidence: 99%

Autophagy guards tendon homeostasis

et al. 2022

Self Cite

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Tendons are vital collagen-dense specialized connective tissues transducing the force from skeletal muscle to the bone, thus enabling movement of the human body. Tendon cells adjust matrix turnover in response to physiological tissue loading and pathological overloading (tendinopathy). Nevertheless, the regulation of tendon matrix quality control is still poorly understood and the pathogenesis of tendinopathy is presently unsolved. Autophagy, the major mechanism of degradation and recycling of cellular components, plays a fundamental role in the homeostasis of several tissues. Here, we investigate the contribution of autophagy to human tendons’ physiology, and we provide in vivo evidence that it is an active process in human tendon tissue. We show that selective autophagy of the endoplasmic reticulum (ER-phagy), regulates the secretion of type I procollagen (PC1), the major component of tendon extracellular matrix. Pharmacological activation of autophagy by inhibition of mTOR pathway alters the ultrastructural morphology of three-dimensional tissue-engineered tendons, shifting collagen fibrils size distribution. Moreover, autophagy induction negatively affects the biomechanical properties of the tissue-engineered tendons, causing a reduction in mechanical strength under tensile force. Overall, our results provide the first evidence that autophagy regulates tendon homeostasis by controlling PC1 quality control, thus potentially playing a role in the development of injured tendons.

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“…207 Signaling by nNOS is important for skeletal muscle homeostasis by regulating many important functions, such as muscle contraction and muscle metabolism. 207,208 In addition, nNOS also contributes to blood flow and oxygen delivery in synergy with eNOS, thus making the metabolic and blood supply demands of the contracting muscle more efficient, 209 making nNOS an essential regulator of skeletal muscle exercise performance, 210 and nNOS may also play a regulatory role in tendon healing. 211 One study evaluated the initial and late (4 and 24 h post-exercise) changes in nNOS following LL-BFRRE compared to LL-FFRE 50 and found a significant increase in the late phase at both 4 and 24 h post-exercise.…”

Section: •-mentioning

confidence: 99%

Hormonal, immune, and oxidative stress responses to blood flow‐restricted exercise

Hjortshoej,

Aagaard,

Storgaard

et al. 2023

Acta Physiologica

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IntroductionHeavy‐load free‐flow resistance exercise (HL‐FFRE) is a widely used training modality. Recently, low‐load blood‐flow restricted resistance exercise (LL‐BFRRE) has gained attention in both athletic and clinical settings as an alternative when conventional HL‐FFRE is contraindicated or not tolerated. LL‐BFRRE has been shown to result in physiological adaptations in muscle and connective tissue that are comparable to those induced by HL‐FFRE. The underlying mechanisms remain unclear; however, evidence suggests that LL‐BFRRE involves elevated metabolic stress compared to conventional free‐flow resistance exercise (FFRE).AimThe aim was to evaluate the initial (<10 min post‐exercise), intermediate (10–20 min), and late (>30 min) hormonal, immune, and oxidative stress responses observed following acute sessions of LL‐BFRRE compared to FFRE in healthy adults.MethodsA systematic literature search of randomized and non‐randomized studies was conducted in PubMed, Embase, Cochrane Central, CINAHL, and SPORTDiscus. The Cochrane Risk of Bias (RoB2, ROBINS‐1) and TESTEX were used to evaluate risk of bias and study quality. Data extractions were based on mean change within groups.ResultsA total of 12525 hits were identified, of which 29 articles were included. LL‐BFRRE demonstrated greater acute increases in growth hormone responses when compared to overall FFRE at intermediate (SMD 2.04; 95% CI 0.87, 3.22) and late (SMD 2.64; 95% CI 1.13, 4.16) post‐exercise phases. LL‐BFRRE also demonstrated greater increase in testosterone responses compared to late LL‐FFRE.ConclusionThese results indicate that LL‐BFRRE can induce increased or similar hormone and immune responses compared to LL‐FFRE and HL‐FFRE along with attenuated oxidative stress responses compared to HL‐FFRE.

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nNOS/GSNOR interaction contributes to skeletal muscle differentiation and homeostasis

Cited by 12 publications

References 40 publications

Can Serum Nitrosoproteome Predict Longevity of Aged Women?

Can Serum Nitrosoproteome Predict Longevity of Aged Women?

Autophagy guards tendon homeostasis

Hormonal, immune, and oxidative stress responses to blood flow‐restricted exercise

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