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Lee, P.C.; Lee, S.Y.; Hong, Soon Ho; Chang, Ho Nam; Park, S.C.

doi:10.1023/a:1021907116361

Cited by 110 publications

(10 citation statements)

References 12 publications

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“…Succinate can be produced anaerobically by naturally isolated bacteria and recombinant organisms, such as rumen bacteria Actinobacillus succinogenes [5], Anaerobiospirillum succiniciproducens [6] and Mannheimia succiniciproducens [7], as well as recombinant Escherichia coli [8]. The rumen bacteria have shown great potential for industrial succinate production, but some of them are potentially pathogenic or strictly anaerobic and difficult to culture [1].…”

Section: Introductionmentioning

confidence: 99%

Engineering of Acetate Recycling and Citrate Synthase to Improve Aerobic Succinate Production in Corynebacterium glutamicum

et al. 2013

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Corynebacterium glutamicum lacking the succinate dehydrogenase complex can produce succinate aerobically with acetate representing the major byproduct. Efforts to increase succinate production involved deletion of acetate formation pathways and overexpression of anaplerotic pathways, but acetate formation could not be completely eliminated. To address this issue, we constructed a pathway for recycling wasted carbon in succinate-producing C. glutamicum. The acetyl-CoA synthetase from Bacillus subtilis was heterologously introduced into C. glutamicum for the first time. The engineered strain ZX1 (pEacsA) did not secrete acetate and produced succinate with a yield of 0.50 mol (mol glucose)−1. Moreover, in order to drive more carbon towards succinate biosynthesis, the native citrate synthase encoded by gltA was overexpressed, leading to strain ZX1 (pEacsAgltA), which showed a 22% increase in succinate yield and a 62% decrease in pyruvate yield compared to strain ZX1 (pEacsA). In fed-batch cultivations, strain ZX1 (pEacsAgltA) produced 241 mM succinate with an average volumetric productivity of 3.55 mM h−1 and an average yield of 0.63 mol (mol glucose) −1, making it a promising platform for the aerobic production of succinate at large scale.

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Section: Introductionmentioning

confidence: 99%

Engineering of Acetate Recycling and Citrate Synthase to Improve Aerobic Succinate Production in Corynebacterium glutamicum

et al. 2013

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“…However, this value is higher than the reported succinic acid volumetric productivity of 0.14 g/L/h from mutant A. niger GCMC-7 with black strap molasses as a substrate, wherein the succinic acid is a secondary product of citric acid fermentation [13]. Bacterial succinic fermentation operates at a higher pH of 6.0 to 6.5 [23,24] while the fungal co-culture process described in this study operated at a relatively lower pH, which reduces the need for pH control at near-neutral levels.…”

Section: Slurry Fermentation Stagementioning

confidence: 68%

“…The highest succinic acid concentration obtained in the batch process under this study (32.06 g/L, after 72 h of slurry fermentation) is comparable to succinic acid levels produced by bacteria utilizing pure fermentable sugars derived from pretreatment and hydrolysis of lignocellulosic substrates. One study obtained 23.8 g/L succinic acid using Anaerobiospirillum succiniciproducens from wood hydrolysate derived from steam explosion pretreatment of oak wood, and supplemented with corn steep liquor in a 32-h batch fermentation [23]. Another study produced 22.5 g/L succinic acid using Actinobacillus succinogenes from hemicellulose hydrolysate produced from acid pretreatment of sugarcane bagasse in a 24-h batch fermentation [24].…”

Section: Slurry Fermentation Stagementioning

confidence: 99%

Direct Succinic Acid Production from Minimally Pretreated Biomass Using Sequential Solid-State and Slurry Fermentation with Mixed Fungal Cultures

et al. 2017

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Conventional bio-based succinic acid production involves anaerobic bacterial fermentation of pure sugars. This study explored a new route for directly producing succinic acid from minimally-pretreated lignocellulosic biomass via a consolidated bioprocessing technology employing a mixed lignocellulolytic and acidogenic fungal co-culture. The process involved a solid-state pre-fermentation stage followed by a two-phase slurry fermentation stage. During the solid-state pre-fermentation stage, Aspergillus niger and Trichoderma reesei were co-cultured in a nitrogen-rich substrate (e.g., soybean hull) to induce cellulolytic enzyme activity. The ligninolytic fungus Phanerochaete chrysosporium was grown separately on carbon-rich birch wood chips to induce ligninolytic enzymes, rendering the biomass more susceptible to cellulase attack. The solid-state pre-cultures were then combined in a slurry fermentation culture to achieve simultaneous enzymatic cellulolysis and succinic acid production. This approach generated succinic acid at maximum titers of 32.43 g/L after 72 h of batch slurry fermentation (~10 g/L production), and 61.12 g/L after 36 h of addition of fresh birch wood chips at the onset of the slurry fermentation stage (~26 g/L production). Based on this result, this approach is a promising alternative to current bacterial succinic acid production due to its minimal substrate pretreatment requirements, which could reduce production costs.

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“…This acid has holds good industrial applications and is used in industries such as, resins, polymer, paints, cosmetics and inks, etc (Vermuri et al, 2002). To date, the economically renewable resources used in succinic acid production reported are cheese whey (Samuelov., 1999;Lee et al, 2000;Lee et al, 2003a;Wan et al, 2008), cane molasses (Agarwal., 2006;Liu et al, 2008), Jerusalem artichoke (Zheng et al, 2010), wheat flour (Du et al, 2008), wood hydrolysate (Lee., 2003b;Kim et al, 2004;Hodge et al, 2009) and corn straw hydrolysate (Zheng et al, 2009). One of the dominating factors in recent organic synthetic routs is green chemistry.…”

Section: Introductionmentioning

confidence: 99%

Succinic Acid As a Green and Bio-Based Catalyst assisted solvent-free one-pot Biginelli synthesis of biologically active 3,4-dihydropyrimidin-2-(1H)-ones/thiones derivatives

Mohamadpour¹,

Feilizadeh²

2019

CMUJNS

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Succinic acid as a bio-based green and versatile catalyst has been employed for one-pot facile three-component Biginelli synthesis of 3,4dihydropyrimidin-2-(1H)-ones/thiones derivatives under solvent-free conditions with high to excellent yields and short reaction times. This sustainable procedure has notable benefits such as easy-to-handle, green, low-cost and non-toxic catalyst, materials available, simple work-up with no necessity of chromatographic purification steps, one-pot and solvent-free conditions. The products have been characterized by melting points and 1 H NMR spectroscopy.

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Cited by 110 publications

References 12 publications

Engineering of Acetate Recycling and Citrate Synthase to Improve Aerobic Succinate Production in Corynebacterium glutamicum

Engineering of Acetate Recycling and Citrate Synthase to Improve Aerobic Succinate Production in Corynebacterium glutamicum

Direct Succinic Acid Production from Minimally Pretreated Biomass Using Sequential Solid-State and Slurry Fermentation with Mixed Fungal Cultures

Succinic Acid As a Green and Bio-Based Catalyst assisted solvent-free one-pot Biginelli synthesis of biologically active 3,4-dihydropyrimidin-2-(1H)-ones/thiones derivatives

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