Untitled

Saxena, Chaitanya; Palai, S. K.; Samantaray, Sanghamitra; Rout, Gyana Ranjan; Das, P.

doi:10.1023/a:1005869404510

Cited by 38 publications

(2 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Thus, BAP when added singly in the medium was the most effective plant growth regulator indicating the cytokinin specificity of nodal explants of A. paniculata for multiple shoot regeneration. The stimulating effect of BAP on multiple shoot formation has been reported earlier for several medicinal and aromatic plant species (Pattnaik and Chand 1996;Saxena et al 1997;Khalafalla and Hattori 1999;Faisal et al 2006).…”

Section: Resultssupporting

confidence: 67%

Rapid in vitro multiplication and plant regeneration from nodal explants of Andrographis paniculata: a valuable medicinal plant

Purkayastha

Sugla

Paul

et al. 2008

In Vitro Cell.Dev.Biol.-Plant

View full text Add to dashboard Cite

A rapid and efficient method for the large-scale propagation of a highly valuable medicinal plant, Andrographis paniculata Nees, through in vitro culture of nodal explants obtained from 15-d-old aseptic seedling has been developed. High frequency direct shoot proliferation was induced in nodal explants cultured on Murashige and Skoog's medium supplemented with 6-benzylaminopurine (BAP). Amongst the various cytokinins tested (BAP, kinetin, thidiazuron and 2-isopentyl adenine), BAP proved to be the most effective. The shoot forming capacity of the nodal explants was influenced by the BAP concentration (1-12.5 μM), and the optimal response was observed at 10 μM BAP, which induced an average of 34 shoots in 94% of the cultures within 4 wk. Significant differences were recorded in terms of average number of shoots per explant (8.6-34.1) among the different concentrations of BAP investigated. Concentrations of all cytokinins tested reach a level that can be considered above the optimum level, as marked by a reduced frequency of shoot proliferation. The multiple shoots obtained on various concentrations of BAP failed to elongate even after transfer to hormone-free MS medium. Elongation of the induced shoots was achieved on MS basal medium supplemented with 1.0 μM GA 3 within 2 wk. A proliferating shoot culture was established by repeatedly subculturing the original nodal explants on shoot multiplication medium after each harvest of the newly formed shoots. The explants retained their morphogenic potential even after three harvests. Therefore, in 90 d, about 60-70 shoots were obtained from a single nodal explant and the nodal explants from primary shoots further regenerated equivalent number of shoots, depicting their high frequency regeneration potential in A. paniculata. Rooting was best induced in 94% of shoots cultured on MS medium supplemented with 2.5 μM indole-3-butyric acid (IBA), within a wk. The plantlets were successfully transferred to soil after hardening with a 92% survival rate. The system is rapid: the initiation of shoot buds to the transplanting of regenerants to soil is completed in 8-9 wk.

show abstract

Section: Resultssupporting

confidence: 67%

Rapid in vitro multiplication and plant regeneration from nodal explants of Andrographis paniculata: a valuable medicinal plant

Purkayastha

Sugla

Paul

et al. 2008

In Vitro Cell.Dev.Biol.-Plant

View full text Add to dashboard Cite

show abstract

“…Nodes, petioles, and leaves of naturally grown and in vitro grown plants of Valeriana were inoculated in MS media supplemented with 5 µM, 10 µM and 15 µM 2,4-D. Saxena et al [17] documented the organogenesis of callus cultures made from Psoralea corylifolia plant's stems, leaves, and petioles. In almost all explant types, callus formation was induced, the magnitude of which varied with the concentration of 2,4-D and the explant type [18] also reported callus formation in leaf explants using 2,4-D).…”

Section: Effect Of 2 4-dmentioning

confidence: 99%

Exploring Regenerative Mechanisms: Comparative Analysis of Callus Induction and Shoot Regeneration in Valeriana jatamansi Jones through In-vitro and In-vivo Cultivation

Prakash,

Shah,

Jangpangi

et al. 2023

IJPSS

View full text Add to dashboard Cite

Valeriana jatamansi Jones, commonly known as Tagar, belongs to the Caprifoliaceae family and is valued for its medicinal properties. This research aims to assess the impact of specific plant growth regulators on different explants obtained from natural and in-vitro cultivated plants. Leaves and nodal explants from both sources were utilized, treated with 2,4-D, NAA, and BAP, and monitored for 30 days. Results indicate widespread callus formation among various explant types. Particularly intriguing is the robust response of in-vitro cultivated explants, in contrast to those from naturally grown plants. Nodal explants exhibited a remarkable tendency for producing multiple shoots, especially in a medium enriched with 20 µM BAP, highlighting BAP's efficacy in promoting shoot regeneration in controlled settings. This study underscores the potential of tissue culture for establishing effective protocols to conserve this medicinal plant, offering insights into sustainable and responsible utilization for informed conservation strategies.

show abstract

Biotechnological Interventions for Conservation and Multiplication of Threatened Medicinal Plants

Rohini

2020

Conservation and Utilization of Threatened Medicinal Plants

View full text Add to dashboard Cite

Untitled

Cited by 38 publications

References 8 publications

Rapid in vitro multiplication and plant regeneration from nodal explants of Andrographis paniculata: a valuable medicinal plant

Rapid in vitro multiplication and plant regeneration from nodal explants of Andrographis paniculata: a valuable medicinal plant

Exploring Regenerative Mechanisms: Comparative Analysis of Callus Induction and Shoot Regeneration in Valeriana jatamansi Jones through In-vitro and In-vivo Cultivation

Biotechnological Interventions for Conservation and Multiplication of Threatened Medicinal Plants

Contact Info

Product

Resources

About