NMR studies of succinoglycan repeating-unit octasaccharides from Rhizobium meliloti and Agrobacterium radiobacter

Chouly, C.; Colquhoun, Ian J.; Jodelet, Anne; York, Gregory M.; Walker, Graham C.

doi:10.1016/0141-8130(96)81846-0

Cited by 57 publications

(20 citation statements)

References 18 publications

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“…In the anomeric region, the absorption band around 796 cm -1 that revealed the possible presence of alpha glycosidic linkages is not observed. Most documented polysaccharides from rhizobia are linked in alpha or beta conformations through several possible glycosidic linkages (Chouly et al, 1995;Fraysse et al, 2003;Bomfeti et al, 2011). However, the difference in chemical composition, type of glycosidic linkage, and degree of polysaccharide branching influence the secondary and tertiary structures of the single chains and their macromolecular assembly, determining the physical properties of the polysaccharide, which are related to the polysaccharides structural or physiological functions (Sutherland, 2001).…”

Section: Ftir Spectroscopy Analysis Of Epsmentioning

confidence: 99%

“…This is composed of a β-1,3, β-1,4, and β-1,6 linked octasaccharide subunit containing galactose at the reducing end and glucose residues in a ratio of 1:7. Also, the repeating unit carries one to two succinyl groups located at the C-6 position of the seventh sugar residue, and a pyruvyl group linked to the eighth sugar residue through a 4, 6-ketal linkage (Chouly et al, 1995;Choi et al, 2012). Sinorhizobial polymers represent only a small fraction of the current polymer market (Donot et al, 2012).…”

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confidence: 99%

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Characterization of Exopolysaccharides Produced by Rhizobia Species

Castellane

Otoboni

Lemos

2015

Rev. Bras. Ciênc. Solo

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increasing attention has been given, over the past decades, to the production of exopolysaccharides (eps) from rhizobia, due to their various biotechnological applications. overall characterization of biopolymers involves evaluation of their chemical, physical, and biological properties; this evaluation is a key factor in understanding their behavior in different environments, which enables researchers to foresee their potential applications. our focus was to study the eps produced by Mesorhizobium huakuii lMG14107, M. loti lMG6125, M. plurifarium lMG11892, Rhizobium giardini bv. giardini h152 t , R. mongolense lMG19141, and Sinorhizobium (= Ensifer) kostiense lMG19227 in a rdM medium with glycerol as a carbon source. these biopolymers were isolated and characterized by reversed-phase high-performance liquid chromatography (rp-hplC), fourier transform infrared (ftir), and nuclear magnetic resonance (nMr) spectroscopies. Maximum exopolysaccharide production was 3.10, 2.72, and 2.50 g l -1 for the strains LMG6125, LMG19227, and LMG19141, respectively. The purified EPS revealed prominent functional reactive groups, such as hydroxyl and carboxylic, which correspond to a typical heteropolysaccharide. the eps are composed primarily of galactose and glucose. Minor components found were rhamnose, glucuronic acid, and galacturonic acid. indeed, from the results of techniques applied in this study, it can be noted that the eps are species-specific heteropolysaccharide polymers composed of common sugars that are substituted by non-carbohydrate moieties. in addition, analysis of these results indicates that rhizobial EPS can be classified into five groups based on ester type, as determined

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Section: Ftir Spectroscopy Analysis Of Epsmentioning

confidence: 99%

mentioning

confidence: 99%

Characterization of Exopolysaccharides Produced by Rhizobia Species

Castellane

Otoboni

Lemos

2015

Rev. Bras. Ciênc. Solo

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“…19,20) Agrobacterium species produce succinoglycans. 18,21) Strain KYM-8 was identiˆed as Agrobacterium tumefaciens; probably, this strain KYM-8 was primarily responsible for APK-78 production and strain KYM-7 acted in cooperation.…”

Section: )mentioning

confidence: 99%

Extracellular Acidic Polysaccharide Production by a Two-membered Bacterial Coculture

Kurata

Yamada²,

Takatsu³

et al. 2003

Bioscience, Biotechnology, and Biochemistry

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“…[2][3][4] The most distinguished feature of the polysaccharide is its high viscosity in the aqueous solution. Moreover, the viscosity of the solution maintains in the high ionic strength condition or acidic condition.…”

Section: Introductionmentioning

confidence: 99%

Consistency Change of Succinoglycan Aqueous Sodium Chloride Solution during Cooling Process

Kaneda

Onodera

2009

J. Soc. Rheol., Jpn

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The rheological properties of succinoglycan aqueous sodium chloride solution were investigated at the various temperatures ranging from 30 to 75 °C. The dynamic modulus of the sample solutions abruptly increased at around 65 °C during the cooling process. The critical temperature (T c ) at which the consistency changed sharply depended on the sodium chloride concentration, namely, T c increased with the salt concentration. Succinoglycan takes a conformation of a semi-flexible single strand chain at above T c . The spatial size of the polysaccharide at high temperature decreased with the salt concentration since the polysaccharide is a polyelectrolyte. The aqueous solution of the polysaccharide became a structured fluid after the cooling process. To make a quantitative characterization of the mechanical property of the structure fluid, the creep curve analysis was employed. The mechanical properties of the structured fluid can be quantitatively described by using 4-elements mechanical model. Moreover, the effect of the salt on the mechanical properties also emerged. Although the consistency of the sample at above T c decreased with the salt concentration, it increased with the salt concentration after cooling below T c . The result revealed that the consistency of the heat denatured succinoglycan aqueous solution was strongly affected with the salt concentration in the system.

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NMR studies of succinoglycan repeating-unit octasaccharides from Rhizobium meliloti and Agrobacterium radiobacter

Cited by 57 publications

References 18 publications

Characterization of Exopolysaccharides Produced by Rhizobia Species

Characterization of Exopolysaccharides Produced by Rhizobia Species

Extracellular Acidic Polysaccharide Production by a Two-membered Bacterial Coculture

Consistency Change of Succinoglycan Aqueous Sodium Chloride Solution during Cooling Process

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