NMR Studies of Renal Metabolism: Regulation of Renal Function by ATP and pHa

Shine, N.; Adam, W. R.; Xuan, Jian Ai; Weiner, Michael W.

doi:10.1111/j.1749-6632.1987.tb32898.x

Cited by 7 publications

(2 citation statements)

References 22 publications

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“…Radda's group introduced the use of 3IP nuclear magnetic resonance (NMR) for the study of renal energy metabo lism [2] and more recently have used these techniques to evaluate the renal metabolic re sponse to hypoxia [3]. Weiner's group [4] have also used 31P-NMR to evaluate the role of ATP turnover in the regulation of renal Na+ trans port and acid-base balance. In these studies, the transfer of magnetization from the y-phos phate of ATP to Pi was used to evaluate the rate of ATP turnover [5].…”

Section: Introductionmentioning

confidence: 99%

31P Nuclear Magnetic Resonance and Saturation Transfer Studies of the Isolated Perfused Rat Kidney

Dowd¹,

Barac‐Nieto²,

Gupta³

et al. 1989

Kidney Blood Press Res

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Previous studies using ³¹P nuclear magnetic resonance (NMR) saturation transfer techniques to quantitate the energy metabolism of the kidney have often resulted in estimates of adenosine triphosphate (ATP) turnover which are much lower than those predicted from the renal oxygen consumption and reasonable values of the P/O ratio. We measured the ATP turnover in isolated perfused kidneys of rats, using ³¹P NMR saturation transfer and a new procedure for quantitation of the intracellular Pi concentration. The estimated turnover rates of ATP were higher than previously reported. The P/O ratios calculated on the basis of these rates of ATP turnover and rates of renal oxygen consumption reported in the literature were within the range of theoretically possible values. Thus, ³¹P NMR saturation transfer can be used to quantitate the ATP turnover in the isolated perfused rat kidney.

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Section: Introductionmentioning

confidence: 99%

31P Nuclear Magnetic Resonance and Saturation Transfer Studies of the Isolated Perfused Rat Kidney

Dowd¹,

Barac‐Nieto²,

Gupta³

et al. 1989

Kidney Blood Press Res

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“…How ever, its non invasiveness and specificity are sig nificant advantages over alternative methods. Most previous studies of kidney by this tech nique have been limited to observations of ''P metabolites and their turnover in perfused or in vivo preparations [1][2][3]. Recently, we and others have measured intracellular sodium ion concentrations in a variety of cell types by 23Na NMR in combination with with a membraneimpermeant anionic paramagnetic shift re agent dysprosium bis(tripolyphosphate), Dy (PPPi)]', that allows discrimination of intraand extracellular N a+ pools .…”

Section: Introductionmentioning

confidence: 99%

23Na, 19F, 35Cl and 31P Multinuclear Nuclear Magnetic Resonance Studies of Perfused Rat Kidney

Gupta

Dowd²,

Spitzer³

et al. 1989

Kidney Blood Press Res

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The concentration of intracellular sodium [Na⁺]_i has been measured in the perfused rat kidney using ²³Na nuclear magnetic resonance (NMR) in combination with the extracellular shift reagent Dy(PPP_i)⁷₂^-. The data show 100% visibility of Na⁺ in interstitial spaces. A measurement of the resonance intensities of intra- and extracellular ²³Na ions along with a knowledge of the extracellular space as a fraction of the total kidney water space yielded an average [Na⁺]_i of 27 ± 2 mM for the kidney at 37°C. After prolonged ischemia [Na⁺]_i rose to approach that in the external medium. In the absence of 5% albumin in the perfusion medium, the linewidth of the ³⁵Cl resonance of an adult kidney (45 Hz) was about twofold larger than that of the medium alone (25 Hz). In contrast, the linewidth of ³⁵Cl resonance of an adult kidney perfused with an albumin-containing medium (82 Hz) was only about 27% of that from the medium alone (300 Hz). We interpret this effect to be due to compartmentation of albumin in the extracellular space such that the interstitial space is not freely accessible to albumin. However, for a developing, immature kidney from a growing animal, perfused with an albumin-containing medium, the linewidth of the ³⁵Cl resonance (233 Hz) was only slightly less than that of the medium alone (300 Hz) indicating a much greater albumin permeability of the capillary walls. ¹⁹F NMR of a perfused adult kidney, loaded with the membrane-impermeant intracellular calcium indicator 5FBAPTA, yielded a value of 256 nM for [Ca²⁺]_i. Induction of ischemia for 10 min caused the [Ca²⁺]_i to rapidly rise to 660 nM, which could not be fully reversed by reperfusion, suggesting irreversible injury.

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Dissociation of decreases in renal cellular energetics and recovery of renal microsomal translation during chronic cyclosporine a administration

Buss¹,

Griffey

1991

Biochemical Pharmacology

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NMR Studies of Renal Metabolism: Regulation of Renal Function by ATP and pHa

Cited by 7 publications

References 22 publications

<sup>31</sup>P Nuclear Magnetic Resonance and Saturation Transfer Studies of the Isolated Perfused Rat Kidney

<sup>31</sup>P Nuclear Magnetic Resonance and Saturation Transfer Studies of the Isolated Perfused Rat Kidney

<sup>23</sup>Na, <sup>19</sup>F, <sup>35</sup>Cl and <sup>31</sup>P Multinuclear Nuclear Magnetic Resonance Studies of Perfused Rat Kidney

Dissociation of decreases in renal cellular energetics and recovery of renal microsomal translation during chronic cyclosporine a administration

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