The proton NMR spectra at 300 MHz of neurotoxin 111 from venom of Naju mossumbicu mossamhicu are reported. By the use of double resonance techniques, pH dependence chemical shifts, isotope labeling technique, and comparison with homologous neurotoxins all proton signals in the aromatic and methyl regions as well as E -C H~ proton signals of some lysine residues have been assigned to individual amino acid residues and their spatial microenvironment has been determined. The results deduced on the solution structure of neurotoxin I11 are in complete agreement with the crystal structure of sea snake erabutoxins as well as with the previously established backbone folding and inter-residue interactions for the Naju naja oxiana short-chain neurotoxin in solution. In addition evidence has been obtained (a) that the conformation of the p turn in the 31 -34 segment depends on the ionization state of the Asp-31 and His-32 side chain groups and (b) that an intricate electrostatic interaction exists in a system of ionogenic groups of the invariant Lys-27, Lys-47, Asp-31, Arg-33, Glu-38 and His-32 residues. These aspects of dynamic conformation are related to an interaction mechanism of a neurotoxin molecule and a nicotinic acetylcholine receptor.Polypeptide toxins are useful probes for studying neuroconductivity processes on the molecular level. Particular attention is being allotted to toxins of sea and amphibious snakes, blocking transmission of the nerve impulse by specifically binding to the acetylcholine receptor of the postsynaptic membrane [I -31.At present the amino acid sequence of about 30 so-called short-chain neurotoxins (60 -62 amino acid residues with four disulphide bonds) from snake venoms has been determined [4]. A crystal structure is determined for two members of this neurotoxin group, erabutoxin a and b of Laticaudu semifasciala [5,6]. The solution conformation of short-chain neurotoxins is being intensively investigated by various physicochemical methods [7-91. Of these the most informative has proved to be NMR spectroscopy in combination with selective chemical modification [lo-221 so as to insert reporter groups into given sites of the protein molecule. Such an approach has made possible determination of the solution conformation of the central loop (residues 23 -38) of the Naja nu@ oxiana neurotoxin I1 [14-181.The absence of suitable reporter groups for the other parts of the molecule and the difficulty of inserting such groups in these parts had been a stumbling block to their NMR conformational characterization. However, molecules with natural reporter groups in the segments of interest could be selected from the extensive homologous series of the 'short' neurotoxin molecules. In particular several such convenient reporter natural ainino acid residues are to be found in the pertinent segments of the Nuju mossambica mossambica neurotoxin I11 molecule (Fig. 1) which we have studied.Of course, making use of the data obtained from the other members of the homologous neurotoxins is possible under the pro...