NMR Structure and Calcium-Binding Properties of the Tellurite Resistance Protein TerD from Klebsiella pneumoniae

Pan, Yun Ru; Lou, Yuan‐Chao; Seven, Alpay B.; Rizo, Josep; Chen, Chinpan

doi:10.1016/j.jmb.2010.11.041

Cited by 18 publications

(29 citation statements)

References 40 publications

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“…Little information is available on the role and function of tdd genes in S. coelicolor, but Tdd8 shows calcium binding properties (http://www.rcsb.org/pdb/explore.do?structureIdϭ3ibz), and it has been suggested that Tdd proteins may play an important role in calcium homeostasis (10,12). The evidence derived from this study supports the hypothesized role of Tdd8 in calcium homeostasis.…”

Section: Discussionsupporting

confidence: 65%

“…In addition to S. coelicolor, other actinobacteria, Firmicutes, and Proteobacteria, as well as primitive eukaryotes such as amoebas and diatoms, have genes with TerD-like motifs. The presence of TerD domain-encoding genes in a wide range of microorganisms suggests that these genes provide a selective advantage in the natural environment, and it has been proposed that tellurite resistance was a consequence of another undetermined biological function and was not the principal role of this class of proteins (10,12).…”

mentioning

confidence: 99%

“…Tdd8 was overproduced even during the transition phase in cells undergoing programed cell death (2). As several Tdd proteins, including Tdd8, exhibit calcium binding properties, it has been suggested that these proteins may play an important role in calcium homeostasis (10,12). Whereas the role of calcium in signal transduction is well established in eukaryotes, it is still elusive in prokaryotes.…”

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confidence: 99%

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AterDDomain-Encoding Gene (SCO2368) Is Involved in Calcium Homeostasis and Participates in Calcium Regulation of a DosR-Like Regulon in Streptomyces coelicolor

et al. 2015

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bAlthough Streptomyces coelicolor is not resistant to tellurite, it possesses several TerD domain-encoding (tdd) genes of unknown function. To elucidate the function of tdd8, the transcriptomes of S. coelicolor strain M145 and of a tdd8 deletion mutant derivative (the ⌬tdd8 strain) were compared. Several orthologs of Mycobacterium tuberculosis genes involved in dormancy survival were upregulated in the deletion mutant at the visual onset of prodiginine production. These genes are organized in a putative redox stress response cluster comprising two large loci. A binding motif similar to the dormancy survival regulator (DosR) binding site of M. tuberculosis has been identified in the upstream sequences of most genes in these loci. A predicted role for these genes in the redox stress response is supported by the low NAD ؉ /NADH ratio in the ⌬tdd8 strain. This S. coelicolor gene cluster was shown to be induced by hypoxia and NO stress. While the tdd8 deletion mutant (the ⌬tdd8 strain) was unable to maintain calcium homeostasis in a calcium-depleted medium, the addition of Ca 2؉ in ⌬tdd8 culture medium reduced the expression of several genes of the redox stress response cluster. The results shown in this work are consistent with Tdd8 playing a significant role in calcium homeostasis and redox stress adaptation.

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Section: Discussionsupporting

confidence: 65%

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confidence: 99%

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confidence: 99%

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AterDDomain-Encoding Gene (SCO2368) Is Involved in Calcium Homeostasis and Participates in Calcium Regulation of a DosR-Like Regulon in Streptomyces coelicolor

et al. 2015

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“…Further research is necessary to understand the role of this important conserved gene in both bacteria and phages. TerC is required for tellurite resistance in E. coli, and several tellurite resistance genes are known; however, the mechanisms by which these proteins function is largely unknown (84)(85)(86). Tellurium oxyanion (TeO 3 2Ϫ ) has been used in the treatment of mycobacterial infections, and resistance is a feature of many pathogenic bacteria (79).…”

Section: Discussionmentioning

confidence: 99%

The Genomes, Proteomes, and Structures of Three Novel Phages That Infect the Bacillus cereus Group and Carry Putative Virulence Factors

Grose

Belnap

Jensen

et al. 2014

J Virol

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This article reports the results of studying three novel bacteriophages, JL, Shanette, and Basilisk, which infect the pathogen Bacillus cereus and carry genes that may contribute to its pathogenesis. We analyzed host range and superinfection ability, mapped their genomes, and characterized phage structure by mass spectrometry and transmission electron microscopy (TEM). The JL and Shanette genomes were 96% similar and contained 217 open reading frames (ORFs) and 220 ORFs, respectively, while Basilisk has an unrelated genome containing 138 ORFs. Mass spectrometry revealed 23 phage particle proteins for JL and 15 for Basilisk, while only 11 and 4, respectively, were predicted to be present by sequence analysis. Structural protein homology to wellcharacterized phages suggested that JL and Shanette were members of the family Myoviridae, which was confirmed by TEM. The third phage, Basilisk, was similar only to uncharacterized phages and is an unrelated siphovirus. Cryogenic electron microscopy of this novel phage revealed a T‫9؍‬ icosahedral capsid structure with the major capsid protein (MCP) likely having the same fold as bacteriophage HK97 MCP despite the lack of sequence similarity. Several putative virulence factors were encoded by these phage genomes, including TerC and TerD involved in tellurium resistance. Host range analysis of all three phages supports genetic transfer of such factors within the B. cereus group, including B. cereus, B. anthracis, and B. thuringiensis. This study provides a basis for understanding these three phages and other related phages as well as their contributions to the pathogenicity of B. cereus group bacteria. IMPORTANCEThe Bacillus cereus group of bacteria contains several human and plant pathogens, including B. cereus, B. anthracis, and B. thuringiensis. Phages are intimately linked to the evolution of their bacterial hosts and often provide virulence factors, making the study of B. cereus phages important to understanding the evolution of pathogenic strains. Herein we provide the results of detailed study of three novel B. cereus phages, two highly related myoviruses (JL and Shanette) and an unrelated siphovirus (Basilisk). The detailed characterization of host range and superinfection, together with results of genomic, proteomic, and structural analyses, reveal several putative virulence factors as well as the ability of these phages to infect different pathogenic species.

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“…Lac. lactis TelB is a homologue of TerD, a metalbinding protein proposed to be involved in calcium signalling (Pan et al, 2011). Overall, DIGE analysis demonstrated that deletion of TrxA influences the expression profiles of proteins implicated in oxidative stress resistance.…”

Section: Changes In Proteome Profiles Of Dtrxa and Dtrxd Mutantsmentioning

confidence: 89%

Two Lactococcus lactis thioredoxin paralogues play different roles in responses to arsenate and oxidative stress

et al. 2015

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NMR Structure and Calcium-Binding Properties of the Tellurite Resistance Protein TerD from Klebsiella pneumoniae

Cited by 18 publications

References 40 publications

AterDDomain-Encoding Gene (SCO2368) Is Involved in Calcium Homeostasis and Participates in Calcium Regulation of a DosR-Like Regulon in Streptomyces coelicolor

AterDDomain-Encoding Gene (SCO2368) Is Involved in Calcium Homeostasis and Participates in Calcium Regulation of a DosR-Like Regulon in Streptomyces coelicolor

The Genomes, Proteomes, and Structures of Three Novel Phages That Infect the Bacillus cereus Group and Carry Putative Virulence Factors

Two Lactococcus lactis thioredoxin paralogues play different roles in responses to arsenate and oxidative stress

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