NMR Methods for Measuring Membrane Transport

Kuchel, Philip W.; Kirk, Kiaran; King, Glenn F.

doi:10.1007/978-1-4615-1863-1_7

Cited by 13 publications

(6 citation statements)

References 205 publications

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“…NMR spectroscopy is now well established as a means of investigating chemical exchange in solution (24,25) or transport across membranes in vesicles or cells (26,27). Several techniques are available for these purposes, including PFG NMR diffusion measurements (24,28); measurements of the relaxation times T 1 , T 1 , and T 2 (29); and magnetization transfer experiments (30,31).…”

Section: Nmr and Bindingmentioning

confidence: 99%

Pulsed field gradient nuclear magnetic resonance as a tool for studying drug delivery systems

Momot

Kuchel

2003

Concepts Magnetic Resonance

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Pulsed field gradient (PFG) nuclear magnetic resonance (NMR) is a versatile method for studying molecular transport and exchange in solution. Stejskal-Tanner diffusion plots can be used to distinguish between chemical species even in the absence of chemical shift or T 1 resolution, provided that certain conditions are satisfied. This can be used advantageously in colloidal drug delivery systems containing two drug populations, free and bound, which often differ only by their diffusion coefficients. We discuss the conditions required for the resolution of the two pools in Stejskal-Tanner plots, as well as the effects of chemical exchange. We also present two practical examples of this analysis: surfactant micelles containing the general anesthetic propofol and lipid/water liquid crystalline mesophase vehicles (cubosomes).

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Section: Nmr and Bindingmentioning

confidence: 99%

Pulsed field gradient nuclear magnetic resonance as a tool for studying drug delivery systems

Momot

Kuchel

2003

Concepts Magnetic Resonance

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“…1, 2) порівняно з інтактною тканиною. Така динаміка показників ЯМР-релаксації протонів свідчить про підвищення рухливості молекул тканинної води, прискорення протонного обміну між кристалічною та вільною фракціями тканинної води, зменшення гідрованого шару (Kuchel et al, 1994). Зростання у нирках щурів із гіпертермією фракції вільної води відбувається за рахунок зменшення частки позаклітинної (Рb) води (рис.…”

Section: результати та їх обговоренняunclassified

Peculiarities of tissue water fractional composition in case of experimental whole-body hyperthermia

Kuznetsova

2015

VDNUBM

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The present study, using proton nuclear magnetic resonance relaxation (NMR) method, was undertaken to compare the water fractional composition in nature tissues (group 1) with those damaged by experimental whole-body hyperthermia (group 2). We measured longitudinal or “spin-lattice” (T1) and transverse or “spin-spin” (T2) relaxation times of protons of tissues (brain, the atria of the heart, the kidneys and the renal cortex) from adult Wistar rats. The differences in T1, T2 and percentage of the intra- and extracellular water between group 1 and 2 were studied to help understand how the water moves in tissues at hyperthermia. The results of this study and the literature data allow to make conclusions about tissue water fractional composition in case of experimental whole-body hyperthermia: (1) fractional composition of water and the distribution of intra- and extracellular fluid in the tissue of the atria of the heart did not change (T1 and T2 relaxation times remained unchanged); (2) the crystalline water fraction increased in brain (longer T1 relaxation rate and shorter T2 relaxation rate). This is obstructing the exchange of protons between free and bound water in brain. Thus, loss of water by brain cells is prevented. The distribution between intra- and extracellular fluid in brain remained unchanged; (3) fraction of free water increased in renal tissue (simultaneous longer T1 and T2 relaxation rates) by reducing the volume of extracellular fluid; (4) thick hydration layer of water (longer T1 relaxation rate, T2 remained unchanged) was formed in the extracellular fluid of renal cortex. This water layer is formed around the sodium ions which concentration is increased in renal cortex tissue of rats from group 2. Аs a result, the amount of fluid secreted by kidneys is reduced, i.e. there is a retention of water in the body. The relevance of our research for the understanding of high temperatures’ adaptation mechanisms is discussed in this paper.

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“…The theory and practice of NMR MT techniques, when applied to cellular systems to study enzyme kinetics and membrane transport, has been discussed in detail before (Kuchel 1990; Kuchel and Chapman 1983;Kuchel et al 1994). However, the aim of the present mini-review is to give a more general description of how membrane transport in human erythrocytes can be quantitatively characterized using MT experiments.…”

Section: Introductionmentioning

confidence: 99%

NMR magnetization-transfer analysis of rapid membrane transport in human erythrocytes

Shishmarev

Kuchel

2016

Biophys Rev

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Nuclear magnetic resonance (NMR) magnetization-transfer (MT) experiments provide a convenient tool for studying rapid sub-second membrane-transport processes in situ in metabolically active cells. These experiments are used with membrane-permeable substances when separate (resolved) NMR signals are observed from their populations inside and outside the cells. Here, we provide a description of the theory and practice of the most common NMR MT experiments that have been used to study membrane-transport processes in human erythrocytes (red blood cells; RBCs). The procedures, involved in the analysis of the experimental data for defining mechanisms of transport, and for estimating values of kinetic parameters in the corresponding mathematical models, are given special attention.

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NMR Methods for Measuring Membrane Transport

Cited by 13 publications

References 205 publications

Pulsed field gradient nuclear magnetic resonance as a tool for studying drug delivery systems

Pulsed field gradient nuclear magnetic resonance as a tool for studying drug delivery systems

Peculiarities of tissue water fractional composition in case of experimental whole-body hyperthermia

NMR magnetization-transfer analysis of rapid membrane transport in human erythrocytes

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