NMR Metabolomics pari passu with Proteomics: Two Relevant Tools for Animal Sciences Combined

Palma, Mariana; Almeida, André M.; Matzapetakis, Manolis

doi:10.1007/978-3-319-69682-9_21

Cited by 1 publication

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“…Currently, the knowledge about the effects of microalgae on the general health status and hepatic metabolism of piglets is practically non-existent. Nuclear Magnetic Resonance (NMR) techniques have proven to be important tools to a comprehensive overview on animal physiology and production 20 and may be useful in the identification of metabolites associated with hepatic metabolism. As mentioned in Madeira et al 21 , there is a pressing need to have such information 22 .…”

Section: Introductionmentioning

confidence: 99%

Impact of dietary Chlorella vulgaris and feed enzymes on health status, immune response and liver metabolites in weaned piglets

Martins

Lopes

Palma

et al. 2022

Sci Rep

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In this study, we analysed the impact of dietary inclusion of Chlorella vulgaris and carbohydrases on general health, redox status, immune response, liver lipids and metabolites in weaned piglets. Forty-four male piglets were allocated into four diets: control (n = 11), CH (control diet with 5% CH, n = 10), CH+R (control diet with 5% CH plus 0.005% Rovabio Excel AP, n = 10), and CH+M (control diet with 5% CH plus 0.01% of a pre-selected four-CAZyme mixture, n = 11). After 15 days of trial, animals were slaughtered and samples of blood and liver collected. Spectrophotometry methods and commercial kits were used to determine blood parameters and gas and liquid chromatography for hepatic fatty acid and chlorophylls profiles, respectively. While total, LDL- and VLDL-cholesterol were increased by CH, the opposite was recorded for HDL-cholesterol (p < 0.001). Piglets fed CH-based diets presented an increase of IgG and a decrease of IgM (p < 0.001) which along with lymphocytes exacerbation contributed for piglets’ survival after weaning. n−6 PUFA were reduced in piglets fed CH and the opposite occurred for n−3 PUFA (p < 0.001), thus benefiting n−6/n−3 ratio in the liver. Chlorophylls amount was not changed by the use of Rovabio or enzymatic mixture. The discriminant analysis applied to hepatic parameters revealed a clear separation between control and CH-based diets but failed to discriminate feed enzymes. Our findings indicate health promoting effects of CH as feed ingredient in piglets’ nutrition at weaning, without negatively impacting on animals’ performance.

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Section: Introductionmentioning

confidence: 99%