NMR-Based Metabolomics Reveals Effects of Water Stress in the Primary and Specialized Metabolisms of Bauhinia ungulata L. (Fabaceae)

Souza, Ana Júlia Borim de; Ocampos, Fernanda Maria Marins; Pulgrossi, Rafael Catoia; Dokkedal, Anne Lígia; Colnago, Luiz Alberto; Cechin, Inês; Saldanha, Luiz Leonardo

doi:10.3390/metabo13030381

Cited by 5 publications

(1 citation statement)

References 69 publications

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“…Traditional communities, such as the Tabeba indigenous people in Ceará state, use B. ungulata leaf tea for its beneficial properties, such as hypoglycemic action. [13,14] Studies have revealed that B. ungulata possesses antioxidant, antimicrobial, larvicidal, anti-inflammatory, cytotoxic, antiproliferative, and antiacetylcholinesterase properties, as well as hypoglycemic effects. [15,16] Plants of the Bauhinia genus exhibit various phytochemicals with antioxidant activities described in the literature, characterized by high levels of phenolic compounds and flavonoids.…”

Section: Introductionmentioning

confidence: 99%

In Vitro and In Silico Antioxidant Activity and Molecular Characterization of Bauhinia ungulata L. Essential Oil

Nunes de Andrade Medeiros,

Conrado Bezerra,

José da Silva

et al. 2024

Chemistry & Biodiversity

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Bauhinia ungulate is an antioxidant medicinal plant used in Brazil to lower glycemic index and alternative treatment for diabetes. Therefore, this study aimed to investigate the antioxidant effects of the essential oil of Bauhinia ungulata (EOBU) collected in Boa Vista, Roraima, Brazil, located in the Amazon region. Gas chromatography was used to characterize the components, and antioxidant assays such as DPPH, TAC, reducing power, Fe2+ chelation, and total phenols were performed. The major constituents were molecularly anchored with the human catalase (CAT) enzyme, and maltol was used as a positive control. Among the 25 revealed components, the main ones were α‐bisabolol (27.2%), β‐Caryophyllene (12.5%), Epi‐γ‐eudesmol (13.6%), α‐Humulene (4.5%), and Caryophyllene Oxide (4.3%). The EOBU showed a TAC value of 618.79 mg of ascorbic acid equivalent, free radical scavenging capacity (DPPH) around 43% and 65%, Fe2+ chelation capacity of 161 ± 6 and 126.7 ± 39.6, for 0.1 mg.mL‐1 and 0.5 mg.mL‐1, respectively. Also, 96.5% of total phenols. α‐bisabolol (‐5.7 ± 0.4 Kcal.mol‐1) and β‐caryophyllene (‐6.1 ± 0.5 Kcal.mol‐1) showed good interaction with CAT compared to Maltol (‐4.4 ± 0.4 Kcal.mol‐1). This study demonstrated that EOBU functions as a potent antioxidant, capable of scavenging free radicals and reducing oxidative stress damage.

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Section: Introductionmentioning

confidence: 99%

In Vitro and In Silico Antioxidant Activity and Molecular Characterization of Bauhinia ungulata L. Essential Oil

Nunes de Andrade Medeiros,

Conrado Bezerra,

José da Silva

et al. 2024

Chemistry & Biodiversity

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The First Comprehensive Chemical Profiling of Vachellia gummifera (Willd.) Kyal. & Boatwr., a Plant with Medicinal Value

Kisiriko,

Noleto‐Dias,

Bitchagno

et al. 2024

Chemistry & Biodiversity

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Vachellia gummifera (Willd.) Kyal. & Boatwr. is a medicinal plant endemic to Morocco that has no documented studies on its chemical composition. In this study, the chemical composition of the water/methanol (4:1) extracts of air‐dried leaf and stem samples of Moroccan V. gummifera was determined using UHPLC‐MS and NMR. In total, over 100 metabolites were identified in our study. Pinitol was the major compound in both the leaf and stem extracts, being significantly more abundant in the former. Asparagine and 3‐hydroxyheteroendrin were the second most abundant compounds in the stem and leaf extracts, respectively, though both compounds were present in each tissue. The other compounds included flavonoids based on quercetin, and phenolic derivatives. Eucomic acid, only identified in the stems and was the major aromatic compound distinguishing the leaf and stem profiles. Quercetin 3‐O‐(6′′‐O‐malonyl)‐β‐D‐glucopyranoside was identified as the major flavonoid in the leaves but was also present in the stems. Other malonylated derivatives that were all flavonol glycosides based on myricetin, kaempferol, and isorhamnetin in addition to quercetin were also identified. This is the first report of eucomic acid and malonylated compounds in Vachellia species. This report provides valuable insights into the chemotaxonomic significance of the Vachellia genus.

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NMR fingerprinting of conventional and genetically modified soybean plants with AtAREB transcription factors

Coutinho,

Facchinatto,

Mertz-Hernning

et al. 2023

Preprint

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Drought stress impacts soybean yield and physiological processes. However, the insertion of the activated form of the AtAREB1 gene in the soybean cultivar BR16, which is sensitive to water deficit improved the drought response of the genetically-modified plants under glasshouse conditions. Thus, in this study used 1H NMR in solution and solid-state NMR to investigate the response of genetically modified soybean overexpressing AREB under water deficiency conditions. We achieved that drought-tolerant soybean yields high content of amino acids isoleucine, leucine, threonine, valine, proline, glutamate, aspartate, asparagine, tyrosine and phenylalanine after twelve days of drought stress conditions, as compared to drought-sensitive soybeans under same conditions. Specific target compounds, including sugars, organic acids and phenolic compounds, were identified as involved in controlling sensitive soybean during the vegetative stage. Solid-state NMR was used to study the impact of drought stress on starch and cellulose content in different soybean genotypes. The findings provide insights into the metabolic adjustments of soybean overexpressing AREB transcription factors in adapting to dry climates. This study presents novel NMR techniques for investigating the metabolome of transgenic soybean plants in response to water deficit. The approach allowed for identification of physiological and morphological changes in drought-resistant and drought-tolerant soybean tissues. The findings indicate that drought stress significantly alters micro and macromolecular metabolism in soybean plants. Differential responses were observed among roots and leaves, as well as drought-tolerant and drought-sensitive cultivars, highlighting the complex interplay between overexpressed transcription factors and drought stress in soybean plants.

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NMR-Based Metabolomics Reveals Effects of Water Stress in the Primary and Specialized Metabolisms of Bauhinia ungulata L. (Fabaceae)

Cited by 5 publications

References 69 publications

In Vitro and In Silico Antioxidant Activity and Molecular Characterization of Bauhinia ungulata L. Essential Oil

In Vitro and In Silico Antioxidant Activity and Molecular Characterization of Bauhinia ungulata L. Essential Oil

The First Comprehensive Chemical Profiling of Vachellia gummifera (Willd.) Kyal. & Boatwr., a Plant with Medicinal Value

NMR fingerprinting of conventional and genetically modified soybean plants with AtAREB transcription factors

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