NMR-based metabolomics profile comparisons to distinguish between embryogenic and non-embryogenic callus tissue of sugarcane at the biochemical level

Mahmud, Iqbal; Shrestha, Bhawana; Boroujerdi, Arezue; Chowdhury, Kamal

doi:10.1007/s11627-015-9687-8

Cited by 27 publications

(22 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Currently, NMR-based 1D 1 H spectral comparisons are widely used in plant science and biotechnology field to decipher clues related with economically and commercially significant crops. 1D 1 H-NMR spectroscopy was used to compare the inner and outer cells of catharanthus roseus calli (Yang et al 2009), healthy and infected C. roseus leaves (Choi et al 2004), to distinguish the metabolic profile differences between embryogenic and non-embryogenic callus tissues of sugarcane (Mahmud et al 2014b), to identify the broad range of compounds such as amino acids, carbohydrates, organic acids and phenolic compounds from callus tissue (Palama et al 2010) and to distinguish between metabolic profiles of powdery mildew resistant and susceptible watermelon (Mahmud et al 2014a). Information in this section suggests that the results presented here using 1D 1 H NMR for identifying metabolites from both tissues of M. oliefera can be useful for building an NMR-based metabolite database.…”

Section: Resultsmentioning

confidence: 99%

“…This makes 1 H NMR a unique technique, which enables the quantification of compounds in relation to any other compound in the spectrum (Dagnino, Schripsema 2005). Proton ( 1 H) NMR spectroscopy is particularly a good choice in plant metabolomics studies given the universal occurrence of protons in organic metabolites (Kim et al 2007; Mahmud et al 2014a; Mahmud et al 2014b; Palama et al 2010). 13 C NMR was used to characterize triacylglycerols of M. oleifera seed oil to identify oleic-vaccenic acid (Vlahov et al 2002) and the same technique is also used for profiling of glucosinolates and phenolics in vegetative and reproductive tissues of M. oleifera L. and M. stenopetala L (Bennett et al 2003).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Tissue-Specific Metabolic Profile Study of Moringa oleifera L. Using Nuclear Magnetic Resonance Spectroscopy

Mahmud

Chowdhury

Boroujerdi

2014

Plant Tissue Cult. & Biotech.

Self Cite

View full text Add to dashboard Cite

Moringa oleifera, an important multipurpose crop, is rich in various phytochemicals: flavonoids, antioxidants, vitamins, minerals and carotenes. The purpose of this study was to profile the groups of metabolites in leaf and stem tissues of M. oleifera. Various sugars, amino acids, and organic acid derivatives were found in all of the M. oleifera tissues with different profiles/peak intensities depending on the tissue. 1D proton nuclear magnetic resonance (NMR) was applied for collecting metabolite spectra. Approximately 30 metabolites with 2 unknown peaks were identified with Chenomx and verified with MMCD databases using carbon data. Among these metabolites, 22 metabolites were identified as common in both leaf and stem tissues. Of the remaining 8 metabolites, 4-aminobutyrate, adenosine, guanosine, tyrosine, and p-cresol were found only in leaf tissues; however, glutamate, glutamine, and tryptophan were found only in stem tissues. Biochemical pathway analysis revealed that 28 identified metabolites were interconnected with 36 different pathways as well as related to different fatty acids and secondary metabolites synthesis biochemical pathways. It is well known that different tissues of M. oleifera have nutritional, medicinal, and therapeutic values; therefore, our main objective is to provide a publicly available M. oliefera tissue specific metabolite database.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Tissue-Specific Metabolic Profile Study of Moringa oleifera L. Using Nuclear Magnetic Resonance Spectroscopy

Mahmud

Chowdhury

Boroujerdi

2014

Plant Tissue Cult. & Biotech.

Self Cite

View full text Add to dashboard Cite

show abstract

“…However, there are considerable variations in other taxa. For example, higher concentrations of Glc, Fru, Suc and starch have been observed in EMs than in NECs of sugarcane (Mahmud et al, 2015), higher levels of mannose have been observed in EMs than in NECs of mangosteen (Maadon et al, 2016), and sucrose was the only metabolite detected by Businge et al (2012) in EMs of Picea abies , but isomaltose accumulated in a line with blocked embryonic development. Therefore, there is no need to analyze further the sugar composition distribution in relation to the tissue type because the composition seems species dependent.…”

Section: Discussionmentioning

confidence: 99%

Cytological, Biochemical and Molecular Events of the Embryogenic State in Douglas-fir (Pseudotsuga menziesii [Mirb.])

Gautier

Label²,

Eliášová

et al. 2019

Front. Plant Sci.

View full text Add to dashboard Cite

Somatic embryogenesis techniques have been developed for most coniferous species, but only using very juvenile material. To extend the techniques’ scope, better integrated understanding of the key biological, physiological and molecular characteristics of embryogenic state is required. Therefore, embryonal masses (EMs) and non-embryogenic calli (NECs) have been compared during proliferation at multiple levels. EMs and NECs originating from a single somatic embryo (isogenic lines) of each of three unrelated genotypes were used in the analyses, which included comparison of the lines’ anatomy by transmission light microscopy, transcriptomes by RNAseq Illumina sequencing, proteomes by free-gel analysis, contents of endogenous phytohormones (indole-3-acetic acid, cytokinins and ABA) by LC-MS analysis, and soluble sugar contents by HPLC. EMs were characterized by upregulation (relative to levels in NECs) of transcripts, proteins, transcription factors and active cytokinins associated with cell differentiation accompanied by histological, carbohydrate content and genetic markers of cell division. In contrast, NECs were characterized by upregulation (relative to levels in EMs) of transcripts, proteins and products associated with responses to stimuli (ABA, degradation forms of cytokinins, phenols), oxidative stress (reactive oxygen species) and carbohydrate storage (starch). Sub-Network Enrichment Analyses that highlighted functions and interactions of transcripts and proteins that significantly differed between EMs and NECs corroborated these findings. The study shows the utility of a novel approach involving integrated multi-scale transcriptomic, proteomic, biochemical, histological and anatomical analyses to obtain insights into molecular events associated with embryogenesis and more specifically to the embryogenic state of cell in Douglas-fir.

show abstract

“…In plant-pathogen interaction studies, metabolomics can unravel pathways hijacked by pathogens and predict resistance mechanisms (López-Gresa et al, 2010 ; Allwood et al, 2012 ; Lee et al, 2016 ). In sugarcane metabolomics has been used to determine profiles related to sucrose accumulation (Bosch et al, 2003 ; Glassop et al, 2007 ); evaluate genotypes with different degrees of susceptibility to orange rust disease (Leme et al, 2014 ); distinguish between embryogenic and non-embryogenic callus tissue (Mahmud et al, 2015 ); and explore potential coproducts besides sucrose (Coutinho et al, 2016 ).…”

Section: Introductionmentioning

confidence: 99%

Metabolome Dynamics of Smutted Sugarcane Reveals Mechanisms Involved in Disease Progression and Whip Emission

et al. 2017

View full text Add to dashboard Cite

Sugarcane smut disease, caused by the biotrophic fungus Sporisorium scitamineum, is characterized by the development of a whip-like structure from the plant meristem. The disease causes negative effects on sucrose accumulation, fiber content and juice quality. The aim of this study was to exam whether the transcriptomic changes already described during the infection of sugarcane by S. scitamineum result in changes at the metabolomic level. To address this question, an analysis was conducted during the initial stage of the interaction and through disease progression in a susceptible sugarcane genotype. GC-TOF-MS allowed the identification of 73 primary metabolites. A set of these compounds was quantitatively altered at each analyzed point as compared with healthy plants. The results revealed that energetic pathways and amino acid pools were affected throughout the interaction. Raffinose levels increased shortly after infection but decreased remarkably after whip emission. Changes related to cell wall biosynthesis were characteristic of disease progression and suggested a loosening of its structure to allow whip growth. Lignin biosynthesis related to whip formation may rely on Tyr metabolism through the overexpression of a bifunctional PTAL. The altered levels of Met residues along with overexpression of SAM synthetase and ACC synthase genes suggested a role for ethylene in whip emission. Moreover, unique secondary metabolites antifungal-related were identified using LC-ESI-MS approach, which may have potential biomarker applications. Lastly, a putative toxin was the most important fungal metabolite identified whose role during infection remains to be established.

show abstract

NMR-based metabolomics profile comparisons to distinguish between embryogenic and non-embryogenic callus tissue of sugarcane at the biochemical level

Cited by 27 publications

References 28 publications

Tissue-Specific Metabolic Profile Study of Moringa oleifera L. Using Nuclear Magnetic Resonance Spectroscopy

Tissue-Specific Metabolic Profile Study of Moringa oleifera L. Using Nuclear Magnetic Resonance Spectroscopy

Cytological, Biochemical and Molecular Events of the Embryogenic State in Douglas-fir (Pseudotsuga menziesii [Mirb.])

Metabolome Dynamics of Smutted Sugarcane Reveals Mechanisms Involved in Disease Progression and Whip Emission

Contact Info

Product

Resources

About