NMR-Based Metabolomic Analysis of Spatial Variation  in Soft Corals

He, Qing; Sun, Ruiqi; Liu, Huijuan; Geng, Zhu‐Feng; Chen, Dawei; Li, Yinping; Han, Jingya; Lin, Wenhan; Shu-shan, DU; Deng, Zhiwei

doi:10.3390/md12041876

Cited by 15 publications

(12 citation statements)

References 44 publications

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“…The use of advanced metabolomic techniques to determine baseline physiologic function in corals and study of their responses to disease and environmental stressors can help identify early diagnostic markers of coral health status (Viant et al 2002(Viant et al , 2003Viant 2007;Tjeerdema 2008). Use of emerging NMR-based metabolomic techniques in soft corals from different geographic locations has provided early evidence of the broad usefulness of these methods (He et al 2014). …”

Section: Introductionmentioning

confidence: 99%

Altered acrylic acid concentrations in hard and soft corals exposed to deteriorating water conditions

Westmoreland

Niemuth

Gracz

et al. 2017

FACETS

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A reliable marker of early coral response to environmental stressors can help guide decision-making to mitigate global coral reef decline by detecting problems before the development of clinically observable disease. We document the accumulation of acrylic acid in two divergent coral taxa, stony small polyp coral (Acropora sp.) and soft coral (Lobophytum sp.), in response to deteriorating water quality characterized by moderately increased ammonia (0.25 ppm) and phosphate (0.15 ppm) concentrations and decreased calcium (360 ppm) concentration, using nuclear magnetic resonance spectroscopy (NMR)-based metabolomic techniques. Changes in acrylic acid concentration in polyp tissues free of zooxanthellae suggest that acrylic acid could be a product of animal metabolism and not exclusively a metabolic by-product of the osmolyte dimethylsulfoniopropionate (DMSP) in marine algae or bacteria. Our findings build on previously documented depletions of acrylic acid in wild coral potentially correlated to temperature stress and provide additional insight into approaches to further characterize the nature of the metabolic accumulation of acrylic acid under controlled experimental conditions.

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Section: Introductionmentioning

confidence: 99%

Altered acrylic acid concentrations in hard and soft corals exposed to deteriorating water conditions

Westmoreland

Niemuth

Gracz

et al. 2017

FACETS

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“…Here, the application of OPLS-DA to 1D 1 H-NMR data (Table 1) revealed an excessive data adjustment in the model that could be attributed to signal overlap in the 1 H-NMR spectra of soft-coral extracts, which was principally due to olefinic protons of diterpenes and the high-field region between 3.2 and 0.8 ppm having a high density of signals due to terpene methyl, methylene, and methine resonances, revealing that soft coral extracts contain large amounts of terpenoids 56 . The noise present in each spectrum of this type of biological sample hinders the alignment and creation of the bins used to reduce the data dimensionality in the NMR spectrum from several thousand points 34 .…”

Section: Discussionmentioning

confidence: 99%

Metabolomic study of soft corals from the Colombian Caribbean: PSYCHE and 1H-NMR comparative analysis

Santacruz

Hurtado

Doohan

et al. 2020

Sci Rep

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Marine organisms have evolved to survive against predators in complex marine ecosystems via the production of chemical compounds. Soft corals (cnidaria, Anthozoa, octocorallia) are an important source of chemically diverse metabolites with a broad spectrum of biological activities. Herein, we perform a comparative study between high-resolution proton nuclear magnetic resonance (1 H-nMR) and pure shift yielded by chirp excitation (PSYCHE) experiments to analyze the metabolic profile of 24 soft corals from the Colombian Caribbean to correlate chemical fingerprints with their cytotoxic activity against three cancer cell lines (human cervical carcinoma (SiHa), human prostatic carcinoma (PC3) and human lung adenocarcinoma (A549)). All data obtained were explored using multivariate analysis using principal components analysis (pcA) and orthogonal partial least squares (opLS) analysis. the results did not show a significant correlation between clusters using 1 H-nMR data in the pcA and opLS-DA models and therefore did not provide conclusive evidence; on the other hand, a metabolomic analysis of pSYcHe data obtained under the same parameters revealed that when a decoupled experiment is performed, it was possible to establish a statistically valid correlation between the chemical composition of soft corals and their cytotoxic activity against the PC3 cancer cell line, where the asperdiol and plexaurolone markers were putatively identified and related to the cytotoxic activity presented by extracts of Plexaurella sp. and Plexaura kukenthali, respectively. these results increase the speed, effectiveness and reliability of analyses for the study of this type of complex matrices. Metabolomics studies allow a complete analysis of a set of metabolites that are the substrates and products of metabolism driving essential cellular functions in a given biological system 1. This research has applications in different fields, such as pharmacology, environmental sciences, chemotaxonomy, nutrition and medicine 2. Recently, metabolomic approaches have allowed the understanding of complex biological systems and the biochemical composition of organisms that live in diverse environments, such as marine areas 3. Goulitquer et al. demonstrated that metabolites are important links between genotype and phenotype and are important for studying several biological processes and for analyzing interactions between organisms within communities via mass spectrometry (MS)-based metabolomics 4. In addition, Mohamed A. Farag et al. compared metabolomics results obtained with liquid chromatography coupled to mass spectrometry (LC-MS) with those obtained by nuclear magnetic resonance (NMR) to investigate the metabolism of 16 Sarcophyton species in the context of their genetic diversity and growth habitats 3. The importance of studying marine invertebrates lies in their extraordinary ability to produce a broad variety of chemical compounds with unique chemical structures that in most cases have been correlated with significant biological activities, which ...

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“…Research in this area has so far been restricted to particular invertebrate species, and there are only a few reports concerning the effects of anthropogenic pressures on the overall bioactivity potential of marine benthic invertebrates, especially in Indonesian tropical coral communities. Previous research has shown that production is negatively related to water quality and positively related to biodiversity of predators and benthic competition (Fleury et al, 2004;Hoover et al, 2008;Januar et al, 2011;He et al, 2014). Thus, the research reported here looked to see if there were any bioactive compound production shifts as reefs degrade and change, and investigated directly whether there is any clear correlation between prevailing environmental conditions and biodiversity, and the bioactivity potential of sponges and soft corals.…”

Section: Introductionmentioning

confidence: 90%

Influence of Anthropogenic Pressures on the Bioactivity Potential of Sponges and Soft Corals in the Coral Reef Environment

Januar

Chasanah²,

Tapiolas³

et al. 2015

Squalen Bull. Marine Fisheries Postharvest Biotech.

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The wealth of marine sponges and soft corals in Indonesian waters represents a rich source of natural products. However, anthropogenic pressures potentially decrease diversity in coral reefs. Presented here are trends for tropical sponge and soft coral biodiversity and their bioactivity potential under the influence of increasing anthropogenic pressures. Samples were collected along transects (near, mid, and far) at Karimunjawa and Seribu Islands Marine National Parks and environmental parameters (salinity, pH, dissolved oxygen (DO), phosphate, nitrate, and ammonia), sponge and soft coral biodiversity, and the bioactivity potential of those organisms (50% Growth Inhibition (GI50) of cancer cell lines H460-Lung, MCF7-Breast, and SF268-CNS) are compared. The environmental conditions and biodiversity were found to be significantly different between groups of sampling sites (P<0.05). Canonical Discriminant Analysis (CDA) revealed DO was the discriminant factor driving the separation between groups (90.1%). Diversity tended to be higher in the Far group with strong and significant relation to DO (R= 0.611, P<0.05) and ammonia (R = -0.812, P<0.05). The CDA also showed that an increase in bioactivity (low % GI50) of sponge and soft coral extracts was related to a canonical function (57.21%) consisting of high DO, high pH, and low nutrients. These findings indicate the production of bioactive compounds is related to diversity and complexity of coral reef systems. Therefore, strategies for marine protection by mitigating the impacts of anthropogenic pressures needs to be optimized in order to conserve the overall environment and sustain its natural bioactivity potential indefinitely.

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NMR-Based Metabolomic Analysis of Spatial Variation in Soft Corals

Cited by 15 publications

References 44 publications

Altered acrylic acid concentrations in hard and soft corals exposed to deteriorating water conditions

Altered acrylic acid concentrations in hard and soft corals exposed to deteriorating water conditions

Metabolomic study of soft corals from the Colombian Caribbean: PSYCHE and 1H-NMR comparative analysis

Influence of Anthropogenic Pressures on the Bioactivity Potential of Sponges and Soft Corals in the Coral Reef Environment

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