NMR-based leaf metabolic profiling of V. planifolia and three endemic Vanilla species from the Peruvian Amazon

Leyva, Vanessa; López, Juan M.; Zevallos-Ventura, Alvaro; Cabrera, Rodrigo; Cañari-Chumpitaz, Cristhian; Toubiana, David; Maruenda, Helena

doi:10.1016/j.foodchem.2021.129365

Cited by 11 publications

(8 citation statements)

References 33 publications

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“…Species with molecular information disclosed are V. pompona , V. palmarum , V. ribeiroi , V. bahiana , V. chamissonis , V. sotoarenasii , V. crenulata , V. imperialis , V. planifolia × V. pompona (hybrid), V. planifolia × V. phaeantha (hybrid), and a wild-type (likely V. odorata from Peru) [ 6 , 71 , 72 , 73 , 74 , 75 , 76 , 77 ]. From the hereby compiled data, the chemical classes of organooxygen compounds, fatty acyls, benzene and substituted derivatives, carboxylic acids and derivatives, saturated hydrocarbons, prenol lipids, and phenols had more than ten representative compounds ( Figure 5 A).…”

Section: Chemical Traits From Vanilla Crop Wrsmentioning

confidence: 99%

“…The ten most frequent flavor descriptors were sweet, fruity, green, fatty, waxy, bitter, vanilla, balsam, caramel, and creamy ( Figure 6 ). By far, V. pompona is the wild-relative vanilla species with most studies related to the elucidation of its metabolites [ 6 , 71 , 72 , 75 , 76 ]. Both V. pompona subsp grandiflora (leaves and green pods) and V. pompona subsp pittieri (green pods), as well as cured V. pompona , were targets of chemical profiling studies ( Supplementary Table S1 ).…”

Section: Chemical Traits From Vanilla Crop Wrsmentioning

confidence: 99%

“…Almost 80% of the total floral perfume was composed of trans -carvone, limonene, and limonene oxide, whereby the first was attributed to the successful attraction of Eulaema bee species by V. pompona [ 41 ]. Additionally, metabolomic studies of the leaves of vanilla WR species were also conducted [ 74 , 76 ]. Through a Nuclear Magnetic Resonance (NMR) analysis, it was observed that the metabolome of in vitro Cymbidium mosaic virus (CymMV) infected vanilla plants was differentially expressed compared to uninfected plants [ 74 ].…”

Section: Chemical Traits From Vanilla Crop Wrsmentioning

confidence: 99%

“…Hence, this study denotes the importance of the elucidation of metabolites to be used as biomarkers for the development of vanilla crops disease control protocols. The NMR-based metabolomic study by Leyva et al [ 76 ] also aimed at the identification of molecular markers from vanilla leaves [ 76 ]. The authors assessed V. planifolia , V. pompona , V. ribeiroi , and V. palmarum differential metabolites expression through a multivariate statistics approach.…”

Section: Chemical Traits From Vanilla Crop Wrsmentioning

confidence: 99%

“…The classes of organic acids, sugars, phenolic glucosides, and amino acids were considered the most relevant markers to differentiate the four species. V. ribeiroi showed significantly higher levels of amino acids (such as alanine, arginine, isoleucine, leucine, phenylalanine, and others); V. palmarum showed significantly higher levels of asparagine, essential in the metabolism of nitrogen transport and storage [ 76 ], and V. pompona , together with V. planifolia , was associated with the highest amounts of glucoside A [ 76 ]. Lastly, these studies indicate meaningful ecological use cases for the elucidation of vanilla WR species metabolites.…”

Section: Chemical Traits From Vanilla Crop Wrsmentioning

confidence: 99%

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Vanilla beyond Vanilla planifolia and Vanilla × tahitensis: Taxonomy and Historical Notes, Reproductive Biology, and Metabolites

Oliveira

Macedo

2022

Plants

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Vanilla is a worldwide cherished condiment, and its volatile market is associated with the so-called “vanilla crisis”. Even though only two species (Vanilla planifolia and V. × tahitensis) are cultivated on a large scale for commercial purposes, the Vanilla genus is comprised of 140 species. The present review article discusses the facets of this crisis, and vanilla crop wild relatives (WRs) are showcased as alternatives to overcome them. Historical, taxonomic, and reproductive biology aspects of the group were covered. Emphasis was given to the metabolic characterization of the vanilla crop WRs, highlighting their main chemical classes and the potential flavor descriptors. Many of these species can produce important flavor compounds such as vanillin, vanillic acid, and acetovanillone, among others. Overall, this review compiles valuable information that can help unravel new chapters of the history of this treasured product by evidencing the biotechnological potential of vanilla crop WRs.

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Section: Chemical Traits From Vanilla Crop Wrsmentioning

confidence: 99%

Section: Chemical Traits From Vanilla Crop Wrsmentioning

confidence: 99%

Section: Chemical Traits From Vanilla Crop Wrsmentioning

confidence: 99%

Section: Chemical Traits From Vanilla Crop Wrsmentioning

confidence: 99%

Section: Chemical Traits From Vanilla Crop Wrsmentioning

confidence: 99%

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Vanilla beyond Vanilla planifolia and Vanilla × tahitensis: Taxonomy and Historical Notes, Reproductive Biology, and Metabolites

Oliveira

Macedo

2022

Plants

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Comprehensive metabolome characterization of leaves, internodes, and aerial roots of Vanilla planifolia by untargeted LC–MS and GC × GC–MS

Beer,

Weinert,

Wellmann

et al. 2024

Phytochemical Analysis

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IntroductionUntargeted metabolomics is a powerful tool that provides strategies for gaining a systematic understanding of quantitative changes in the levels of metabolites, especially when combining different metabolomic platforms. Vanilla is one of the world's most popular flavors originating from cured pods of the orchid Vanilla planifolia. However, only a few studies have investigated the metabolome of V. planifolia, and no LC–MS or GC–MS metabolomics studies with respect to leaves have been performed.ObjectiveThe aim of the study was to comprehensively characterize the metabolome of different organs (leaves, internodes, and aerial roots) of V. planifolia.Material and MethodsCharacterization of the metabolome was achieved using two complementary platforms (GC × GC–MS, LC‐QToF‐MS), and metabolite identification was based on a comparison with in‐house databases or curated external spectral libraries.ResultsIn total, 127 metabolites could be identified with high certainty (confidence level 1 or 2) including sugars, amino acids, fatty acids, organic acids, and amines/amides but also secondary metabolites such as vanillin‐related metabolites, flavonoids, and terpenoids. Ninty‐eight metabolites showed significantly different intensities between the plant organs. Most strikingly, aglycons of flavonoids and vanillin‐related metabolites were elevated in aerial roots, whereas its O‐glycoside forms tended to be higher in leaves and/or internodes. This suggests that the more bioactive aglycones may accumulate where preferably needed, e.g. for defense against pathogens.ConclusionThe results derived from the study substantially expand the knowledge regarding the vanilla metabolome forming a valuable basis for more targeted investigations in future studies, e.g. towards an optimization of vanilla plant cultivation.

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Metabolomics in Fundamental Plant Research

Ordaz-Ortiz,

Arroyo-Silva,

Guerrero-Esperanza

2023

Metabolomics

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NMR-based leaf metabolic profiling of V. planifolia and three endemic Vanilla species from the Peruvian Amazon

Cited by 11 publications

References 33 publications

Vanilla beyond Vanilla planifolia and Vanilla × tahitensis: Taxonomy and Historical Notes, Reproductive Biology, and Metabolites

Vanilla beyond Vanilla planifolia and Vanilla × tahitensis: Taxonomy and Historical Notes, Reproductive Biology, and Metabolites

Comprehensive metabolome characterization of leaves, internodes, and aerial roots of Vanilla planifolia by untargeted LC–MS and GC × GC–MS

Metabolomics in Fundamental Plant Research

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