NMR assignment of the immune mapped protein 1 (IMP1) homologue from Plasmodium falciparum

Abstract

Abbreviations IMP1Immune AbstractPlasmodium falciparum is responsible for causing cerebral malaria in humans. IMP1 is an immunogenic protein, present in the parasite, which has been shown to induce an immune response against apicomplexan parasites in a species-specific manner. Here, we report the complete NMR assignments of PfIMP1.

Toxoplasma gondii immune mapped protein 1 is anchored to the inner leaflet of the plasma membrane and adopts a novel protein fold

“…The quality of the NMR spectra for PfIMP2 facilitated the complete sequential assignment using heteronuclear multidimensional NMR methods [12]. Subsequent side-chain assignment facilitated an automated analysis of the nuclear Overhauser data and the solution structure determination using the ARIA protocol [13].…”

“…NMR spectra were recorded at 303 K on Bruker DRX600 and DRX800 spectrometers equipped with cryo-probes. The chemical shifts of 1 HN, 15 N, 13 Cα, 13 Cβ and 13 CO cross peaks were assigned using CBCA(CO)NH, HNCACB, HNCO and HN(CA)CO experiments[12, 32]. The aliphatic and aromatic side chain 1 H and 13 C assignments were obtained from HBHA (CBCACO)NH, HCCH-TOCSY, (H)CC(CO)NHTOCSY and 1 H– 13 C NOESY-HMQC spectra.…”