NMR analysis of the O‐antigen polysaccharide from <i>Escherichia coli</i> strain F171

Fundin, Johan; Weintraub, Andrej; Xu, Jianguo; Widmalm, Göran

doi:10.1002/mrc.1162

Cited by 2 publications

(3 citation statements)

References 16 publications

(5 reference statements)

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“…The four genes encoding dTDP-α-L-rhamnose biosynthesis enzymes were highly conserved in the three strains, consistent with the presence of α-L-rhamnose in the O16 and O25 antigen repeat unit expressed by MG1655 and EC958, respectively [56]–[58]. The dTDP-α-L-rhamnose biosynthesis pathway starts from α-D-glucose-1-phosphate to produce dTDP-α-L-rhamnose via the catalysis of RmlA (to make dTDP-α-D-glucose), RmlB (to make dTDP-4-dehydro-6-deoxy-α-D-glucose), RmlC (to make dTDP-4-dehydro-6-deoxy-β-mannose) and RmlD (to make dTDP-α-L-rhamnose), respectively.…”

Section: Resultssupporting

confidence: 57%

“…(B) Predicted function of the four glycosyl transferases in the biosynthesis of the O25b repeat unit [57], [58]. (C) LPS gels showing changes in O-antigen structures corresponding to mutations in each gene.…”

Section: Resultsmentioning

confidence: 99%

“…However, EC958 lacks the biosynthesis genes for UDP-FucNAc, a component of O25 antigen unit. If, based on the cross reaction of antiserum against the O25 antigen with O25b expressing cells, we assume that EC958 has the same O-antigen repeat unit as the O25 determined from previous studies [57], [58], then EC958 must possess a novel mechanism for the synthesis or uptake of UDP-FucNAc.…”

Section: Discussionmentioning

confidence: 99%

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The Serum Resistome of a Globally Disseminated Multidrug Resistant Uropathogenic Escherichia coli Clone

et al. 2013

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Escherichia coli ST131 is a globally disseminated, multidrug resistant clone responsible for a high proportion of urinary tract and bloodstream infections. The rapid emergence and successful spread of E. coli ST131 is strongly associated with antibiotic resistance; however, this phenotype alone is unlikely to explain its dominance amongst multidrug resistant uropathogens circulating worldwide in hospitals and the community. Thus, a greater understanding of the molecular mechanisms that underpin the fitness of E. coli ST131 is required. In this study, we employed hyper-saturated transposon mutagenesis in combination with multiplexed transposon directed insertion-site sequencing to define the essential genes required for in vitro growth and the serum resistome (i.e. genes required for resistance to human serum) of E. coli EC958, a representative of the predominant E. coli ST131 clonal lineage. We identified 315 essential genes in E. coli EC958, 231 (73%) of which were also essential in E. coli K-12. The serum resistome comprised 56 genes, the majority of which encode membrane proteins or factors involved in lipopolysaccharide (LPS) biosynthesis. Targeted mutagenesis confirmed a role in serum resistance for 46 (82%) of these genes. The murein lipoprotein Lpp, along with two lipid A-core biosynthesis enzymes WaaP and WaaG, were most strongly associated with serum resistance. While LPS was the main resistance mechanism defined for E. coli EC958 in serum, the enterobacterial common antigen and colanic acid also impacted on this phenotype. Our analysis also identified a novel function for two genes, hyxA and hyxR, as minor regulators of O-antigen chain length. This study offers novel insight into the genetic make-up of E. coli ST131, and provides a framework for future research on E. coli and other Gram-negative pathogens to define their essential gene repertoire and to dissect the molecular mechanisms that enable them to survive in the bloodstream and cause disease.

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Section: Resultssupporting

confidence: 57%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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The Serum Resistome of a Globally Disseminated Multidrug Resistant Uropathogenic Escherichia coli Clone

et al. 2013

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Structure and genetics ofEscherichia coliO antigens

Liu

Furevi

Perepelov

et al. 2019

FEMS Microbiology Reviews

173

184

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Escherichia coli includes clonal groups of both commensal and pathogenic strains, with some of the latter causing serious infectious diseases. O antigen variation is current standard in defining strains for taxonomy and epidemiology, providing the basis for many serotyping schemes for Gram-negative bacteria. This review covers the diversity in E. coli O antigen structures and gene clusters, and the genetic basis for the structural diversity. Of the 187 formally defined O antigens, six (O31, O47, O67, O72, O94 and O122) have since been removed and four (O14, O34, O89 and O144) strains do not produce any O antigen. Therefore, structures are presented for 176 of the 181 E. coli O antigens, some of which include subgroups. Most (93%) of these O antigens are synthesized via the Wzx/Wzy pathway, 11 via the ABC transporter pathway, with O20, O57 and O60 still uncharacterized due to failure to find their O antigen gene clusters. Biosynthetic pathways are given for 38 of the 49 sugars found in E. coli O antigens, and several pairs or groups of the E. coli antigens that have related structures show close relationships of the O antigen gene clusters within clades, thereby highlighting the genetic basis of the evolution of diversity.

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NMR analysis of the O‐antigen polysaccharide from Escherichia coli strain F171

Cited by 2 publications

References 16 publications

The Serum Resistome of a Globally Disseminated Multidrug Resistant Uropathogenic Escherichia coli Clone

The Serum Resistome of a Globally Disseminated Multidrug Resistant Uropathogenic Escherichia coli Clone

Structure and genetics ofEscherichia coliO antigens

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