NLRP3 inflammasome is responsible for Hantavirus inducing interleukin-1β in THP-1 cells

Ye, Wei; Lei, Yingfeng; Yu, Mengmeng; Xu, Yongni; Cao, Mengyuan; Lan, Yu; Zhang, Liang; Li, Puyuan; Bai, Wentao; Zhang, Xu; Zhang, Fanglin

doi:10.3892/ijmm.2015.2162

Cited by 16 publications

(14 citation statements)

References 69 publications

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“…Cleaved caspase-1 modulates the maturation of pro-inflammatory cytokines, including IL-1β and IL-18 ( 43 ). Previously, NLRP3 caused renal injury has been reported ( 44 ). We found that TXNIP and NLRP3 signaling pathway was upregulated for LPS treatment, followed by ASC and caspase-1 cleavage increase, which was in line with the inflammation response.…”

Section: Discussionmentioning

confidence: 98%

Fisetin administration improves LPS-induced acute otitis media in mouse in vivo

Chen

Huang

2018

Int J Mol Med

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Acute otitis media is one of the most common infectious diseases worldwide in spite of the widespread vaccination. The present study was conducted to explore the effects of fisetin on mouse acute otitis media models. The animal models were established by lipopolysaccharide (LPS) injection into the middle ear of mice via the tympanic membrane. Fisetin was administered to mice for ten days through intragastric administration immediate after LPS application. Hematoxylin and eosin (H&E) staining was performed and the pro-inflammatory cytokines, including interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), IL-6 and VEGF, were measured through enzyme-linked immunosorbent assay (ELISA) method and RT-qPCR analysis. Toll-like receptor 4 (TLR4)/nuclear factor-κB (NF-κB) signaling pathway was detected by immunoblotting assays. Reactive oxygen species (ROS) generated levels were determined through assessment of anti-oxidants, and TXNIP/MAPKs signaling pathways were explored to reveal the possible molecular mechanism for acute otitis media progression and the function of fisetin. Fisetin reduced mucosal thickness caused by LPS. In fisetin-treated animals, pro-inflammatory cytokine release was downregulated accompanied with TLR4/NF-κB inactivation. ROS production was significantly decreased in comparison to the LPS-treated group. The TXNIP/MAPKs signaling pathway was inactivated for fisetin treatment in LPS-induced mice with acute otitis media. The above results indicated that fisetin improved acute otitis media through inflammation and ROS suppression via inactivating TLR4/NF-κB and TXNIP/MAPKs signaling pathways.

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Section: Discussionmentioning

confidence: 98%

Fisetin administration improves LPS-induced acute otitis media in mouse in vivo

Chen

Huang

2018

Int J Mol Med

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“…The improved plaque formation test is dependent on the low pH-induced cytopathic effects of hantavirus but is time-consuming and has low reproducibility. The most widely adopted approach to test hantavirus titers (especially for HTNV) is the TCID50 (50% tissue culture infective dose) calculation using ELISA as previously reported by our group (Xu et al, 2002; Cheng et al, 2014; Jiang et al, 2015; Ye et al, 2015a,b; Ying et al, 2016); however, virus propagation in Vero E6 cells takes at least 10 days. All the reported detective measurements have insurmountably objective drawbacks, such as high demanding experimental conditions for qRT-PCR and expensive apparatus and labware for FCM, which limits their applicability (Wan et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

In-Cell Western Assays to Evaluate Hantaan Virus Replication as a Novel Approach to Screen Antiviral Molecules and Detect Neutralizing Antibody Titers

Chen

et al. 2017

Front. Cell. Infect. Microbiol.

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Hantaviruses encompass rodent-borne zoonotic pathogens that cause severe hemorrhagic fever disease with high mortality rates in humans. Detection of infectious virus titer lays a solid foundation for virology and immunology researches. Canonical methods to assess viral titers rely on visible cytopathic effects (CPE), but Hantaan virus (HTNV, the prototype hantavirus) maintains a relatively sluggish life cycle and does not produce CPE in cell culture. Here, an in-cell Western (ICW) assay was utilized to rapidly measure the expression of viral proteins in infected cells and to establish a novel approach to detect viral titers. Compared with classical approaches, the ICW assay is accurate and time- and cost-effective. Furthermore, the ICW assay provided a high-throughput platform to screen and identify antiviral molecules. Potential antiviral roles of several DExD/H box helicase family members were investigated using the ICW assay, and the results indicated that DDX21 and DDX60 reinforced IFN responses and exerted anti-hantaviral effects, whereas DDX50 probably promoted HTNV replication. Additionally, the ICW assay was also applied to assess NAb titers in patients and vaccine recipients. Patients with prompt production of NAbs tended to have favorable disease outcomes. Modest NAb titers were found in vaccinees, indicating that current vaccines still require improvements as they cannot prime host humoral immunity with high efficiency. Taken together, our results indicate that the use of the ICW assay to evaluate non-CPE Hantaan virus titer demonstrates a significant improvement over current infectivity approaches and a novel technique to screen antiviral molecules and detect NAb efficacies.

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“…Similarly, the NLRP3 inflammasome is capable of recognizing RVFV and HNTV infection (75,76). RVFV alone could barely promote the activation of the NLRP3 inflammasome.…”

Section: Pyroptosis In Anti-sftsv Immunitymentioning

confidence: 99%

Unraveling the Underlying Interaction Mechanism Between Dabie bandavirus and Innate Immune Response

Zhou

2021

Front. Immunol.

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The genus Bandavirus consists of seven tick-borne bunyaviruses, among which four are known to infect humans. Dabie bandavirus, severe fever with thrombocytopenia syndrome virus (SFTSV), poses serious threats to public health worldwide. SFTSV is a tick-borne virus mainly reported in China, South Korea, and Japan with a mortality rate of up to 30%. To date, most immunology-related studies focused on the antagonistic role of SFTSV non-structural protein (NSs) in sequestering RIG-I-like-receptors (RLRs)-mediated type I interferon (IFN) induction and type I IFN mediated signaling pathway. It is still elusive whether the interaction of SFTSV and other conserved innate immune responses exists. As of now, no specific vaccines or therapeutics are approved for SFTSV prevention or treatments respectively, in part due to a lack of comprehensive understanding of the molecular interactions occurring between SFTSV and hosts. Hence, it is necessary to fully understand the host-virus interactions including antiviral responses and viral evasion mechanisms. In this review, we highlight the recent progress in understanding the pathogenesis of SFTS and speculate underlying novel mechanisms in response to SFTSV infection.

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NLRP3 inflammasome is responsible for Hantavirus inducing interleukin-1β in THP-1 cells

Cited by 16 publications

References 69 publications

Fisetin administration improves LPS-induced acute otitis media in mouse in vivo

Fisetin administration improves LPS-induced acute otitis media in mouse in vivo

In-Cell Western Assays to Evaluate Hantaan Virus Replication as a Novel Approach to Screen Antiviral Molecules and Detect Neutralizing Antibody Titers

Unraveling the Underlying Interaction Mechanism Between Dabie bandavirus and Innate Immune Response

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