NLRP3 inflammasome activation in human peripheral blood mononuclear cells induced by venoms secreted PLA2s

Silva, Milena Daniela Souza; Lopes, Jéssica Amaral; Paloschi, Mauro Valentino; Boeno, Charles Nunes; Rego, Cristina Matiele Alves; Sousa, Ortência de Oliveira; Santana, Hallison Mota; Reis, Valdison Pereira dos; Serrath, Suzanne Nery; Setúbal, Sulamita da Silva; Lima, Anderson Maciel de; Soares, Andreimar Martins; Zuliani, Juliana Pavan

doi:10.1016/j.ijbiomac.2022.01.107

Cited by 12 publications

(1 citation statement)

References 35 publications

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“…Absorbances were conducted in BioTek Synergy HT MultiDetection (Winooski, VT) with 340 nm. Results were expressed in U/L, according to Pereira dos Reis et al[16] and Silva et al[26]. (350 nM) and CCCP3 (240 nM) for 30 min, at 37 °C in a humid atmosphere with 5% CO 2 .…”

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confidence: 99%

Light Emitting Diode Photobiomodulation Enhances Oxidative Redox Capacity in Murine Macrophages Stimulated with Bothrops jararacussu Venom and Isolated PLA2s

Reis

Setúbal

Ferreira

et al. 2022

BioMed Research International

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Photobiomodulation therapy associated with conventional antivenom treatment has been shown to be effective in reducing the local effects caused by bothropic venoms in preclinical studies. In this study, we analyzed the influence of photobiomodulation using light emitting diode (LED) on the oxidative stress produced by murine macrophages stimulated with Bothrops jararacussu venom and it isolated toxins BthTX-I and BthTX-II. Under LED treatment, we evaluated the activity of the antioxidant enzymes catalase, superoxide dismutase, and peroxidase as well as the release of hydrogen peroxide and the enzyme lactate dehydrogenase. To investigate whether NADPH oxidase complex activation and mitochondrial pathways could contribute to hydrogen peroxide production by macrophages, we tested the effect of two selective inhibitors, apocynin and CCCP3, respectively. Our results showed that LED therapy was able to decrease the production of hydrogen peroxide and the liberation of lactate dehydrogenase, indicating less cell damage. In addition, the antioxidant enzymes catalase, superoxide dismutase, and peroxidase increased in response to LED treatment. The effect of LED treatment on macrophages was inhibited by CCCP3, but not by apocynin. These findings show that LED photobiomodulation treatment protects macrophages, at least in part, by reducing oxidative stress caused B. jararacussu venom and toxins.

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confidence: 99%