Nizatidine accelerates gastric emptying of a solid meal in rats

Abstract

Nizatidine, a new histamine-2-receptor antagonist, stimulates gastrointestinal motility in dogs and gastric emptying of liquids in rats. Effect of nizatidine on gastric emptying of a solid meal was investigated using a novel gastric emptying model in rats. Male Wistar rats (weighing 200-300 g) were supplied with powdered food containing 30 w/w% barium 14 hr before the beginning of the experiment and x-ray photography of rat stomach was taken under light ether anesthesia. Gastric emptying was assessed by percentage of a decrease in area 30 min after drug was injected intraperitoneally. There was a positive correlation between the area of the gastric outline and the weight of the gastric contents (r = 0.94, P < 0.01). Ether anesthesia itself did not affect gastric emptying. Nizatidine increased gastric emptying dose-dependently (emptied percentage; vehicle: 4.9 +/- 1.5%, 1 mg/kg: 7.2 +/- 0.4%, 3 mg/kg: 10.4 +/- 2.0%, 10 mg/kg: 16.7 +/- 4.9%, 30 mg/kg: 25.7 +/- 7.4%). N-Desmethyl nizatidine (NDM) also stimulated gastric emptying, but nizatidine S-oxide, cimetidine, an famotidine had no significant effects on gastric emptying. Nizatidine and neostigmine, but not NDM, at a subthreshold dose accelerated gastric emptying treated with a low dose of acetylcholine (0.1 mg/kg). Atropine (2 mg/kg, -30 min) did not modulate the gastroprokinetic action of nizatidine, but blocked that of NDM. These findings suggest that this noninvasive method may allow measurement of gastric emptying of solids accurately and that nizatidine and NDM facilitate gastric emptying probably mediated by a direct and/or an indirect (acetylcholinesterase inhibition) cholinergic mechanism.

Effect of Barley Endoprotease EP-B2 on Gluten Digestion in the Intact Rat

“…A number of studies (Robert et al, 1991;Matsuda et al, 1993;Burton-Freeman et al, 1997;Izbeki et al, 2001;Overhaus et al, 2004;Turan and Ozdemir, 2004) have exploited the intrinsic similarities between gastrointestinal metabolism of food in rats and hu-mans. Rats may be an effective model for gluten digestion because they are able to eat and digest a significant level of food (on the order of several grams, which would represent a physiological gluten load for a Celiac patient) in a suitable time frame (i.e., in approximately 5-8 h) (Kaneko et al, 1995).…”

Nizatidine ameliorates gastroparesis in Parkinson's disease: A pilot study

“…NZT is a selective histamine H2‐receptor antagonist, originally manufactured as a drug to treat peptic ulcer . However, in contrast to other H2‐receptor antagonists (cimetidine, famotidine, or ranitidine, famotidine) that lack prokinetic properties, NZT has a clear prokinetic effect on the gut in experimental animals and in humans . Therefore, while inhibiting gastric acid secretion via H2‐receptor antagonism, NZT promotes gastric motility .…”

Nizatidine Improves Clinical Symptoms and Gastric Emptying in Patients with Functional Dyspepsia Accompanied by Impaired Gastric Emptying

“…Nizatidine enhances the gastric emptying of liquids in patients with gastric or duodenal ulcer, as measured by acetaminophen method [18]. Kaneko et al [35] have also reported that nizatidine, but not cimetidine or famotidine, dose-dependently accelerated gastric emptying of barium-containing solid meal in rats. In contrast, Koskenpato et al [36] have reported that nizatidine decreases the gastric emptying rate of solids without having a significant effect on symptoms or quality of life in FD patients.…”