Phycoerythrin (PE) is a natural water-soluble
pigment protein with
characteristic phycobilins and is sensitive to thermal and light environmental
changes. In this study, PE was extracted from Porphyra
haitanensis and PE–oligochitosan complexes (POC) were fabricated by a
self-assembly approach. The effects of cationic oligochitosan on the
binding interaction, structure, size distribution, and color stability
of PE were evaluated. The stoichiometric number n was calculated to be 21.67 ± 2.65 (oligochitosan/PE) and the
binding constant K was (6.47 ± 0.48) × 105 M–1. Cationic oligochitosan could electrostatically interact
with PE and affect the PE structure by increasing the α-helix
content. In addition, high concentrations of oligochitosan led to
the formation of dense phycoerythrin protein granules. Moreover, at
a reaction ratio of 20.0:1 (oligochitosan/PE), being approximately
the predicted stoichiometric number n, the thermal
stability (40–80 °C), natural light stability, and ultraviolet
light irradiation (254 nm) stability of the POC were improved. This
study provides an approach to reduce the susceptibility of PE upon
environmental changes by forming a stable self-assembly complex, which
will promote the application of PE as a natural pigment protein in
food and chemical applications.