Nitroxides XLVIII: A study of the interaction between bovine serum albumin and a modified steroid by electron spin resonance

Interaction of purified human transcortin with spin labelled steroids

“…for the investigation of steroid-protein interaction using a synthetic steroidal nitroxide and bovine serum albumin as a model protein [6]. Recently, similar results have been reported using the same protein and a spin labelled androstanol [7,8] and the principle of spin immunoassay has been extended to progesterone and testosterone using the corresponding antibodies [9,10].…”

“…Quantitative measurements can be made to determine binding parameters under various experimental conditions (e.g. thermodynamic studies at variable temperatures) [6]. Fig.2 (5) shows the ESR spectrum when the interaction of F-NO with human serum albumin (HSA, Calbiochem.)…”

Synthesis and characterization of a novel cholesterol nitroxide spin label. Application to the molecular organization of human high-density lipoprotein

“…This substance likely was formed by addition of isohexylmagnesium bromide to the ketone group of 21 (as the salt), producing 22, which then could undergo a spontaneous ^-elimination reaction to give nitrone 24. Addition of Grignard reagent to 24 followed by an oxidation of the intermediate A-hydroxy compound during workup would give 25. Probably the low yield in which 25 is formed essentially precluded its isolation from the small-scale synthesis of 23 from 21 discussed above.…”