Nitroxidergic system in human trigeminal ganglia neurons: A quantitative evaluation

Borsani, Elisa; Giovannozzi, Sara; Boninsegna, Ramon; Rezzani, Rita; Labanca, Mauro; Tschabitscher, Manfred; Rodella, Luigi Fabrizio

doi:10.1016/j.acthis.2009.04.006

Cited by 12 publications

(6 citation statements)

References 44 publications

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“…For the analysis, the immunopositivity was evaluated at a final 200× magnification using an optical microscope (Olympus, Hamburg, Germany). Digitally fixed images were analysed using an image analyser (Image Pro-Plus, Milan, Italy) by researchers unaware of the group assignment and were calculated as integrated optical density (IOD) according to Borsani and colleagues [ 47 ]. The analysis has been performed on five sections for each sample, evaluating six random fields with the same area for each section.…”

Section: Methodsmentioning

confidence: 99%

Single Administration of Melatonin Modulates the Nitroxidergic System at the Peripheral Level and Reduces Thermal Nociceptive Hypersensitivity in Neuropathic Rats

Borsani

Buffoli

Bonazza

et al. 2017

IJMS

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Neuropathic pain is a severe condition with unsatisfactory treatments. Melatonin, an indolamine, seems to be a promising molecule suitable for this purpose due to its well-known anti-inflammatory, analgesic, and antioxidant effects, as well as its modulation of the nitroxidergic system. Nevertheless, the data on its mechanism of action and potentialities are currently insufficient in this pathology, especially at the peripheral level. Thus, this work evaluated the effect of a single administration of melatonin in an established mononeuropathy pain model that monitors the behaviour and the changes in the nitroxidergic system in dorsal root ganglia and skin, which are affected by nervous impairment. Experiments were carried out on Sprague Dawley rats subdivided into the sham operated (control) and the chronic constriction injured animals, a model of peripheral neuropathic pain on sciatic nerve. Single administrations of melatonin (5–10 mg/kg) or vehicle were injected intraperitoneally on the 14th day after surgery, when the mononeuropathy was established. The animals were behaviourally tested for thermal hyperalgesia. The dorsal root ganglia and the plantar skin of the hind-paws were removed and processed for the immunohistochemical detection of neuronal and inducible nitric oxide synthases. The behavioural results showed an increase of withdrawal latency during the plantar test as early as 30 min after melatonin administration. The immunohistochemical results indicated a modulation of the nitroxidergic system both at dorsal root ganglia and skin level, permitting speculate on a possible mechanism of action. We showed that melatonin may be a possible therapeutic strategy in neuropathic pain.

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Section: Methodsmentioning

confidence: 99%

Single Administration of Melatonin Modulates the Nitroxidergic System at the Peripheral Level and Reduces Thermal Nociceptive Hypersensitivity in Neuropathic Rats

Borsani

Buffoli

Bonazza

et al. 2017

IJMS

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“…Furthermore, there is evidence about up-regulation of iNOS in culture conditions [41]. In contrast to the other isoforms, the neuronal NOS is present in all parts of the trigeminal system and might therefore be the major source of endogenous NO in the trigeminal nerve [3,38,[42][43][44]. Taken together, both gasotransmitters can be produced endogenously in the trigeminal system and co-localization of their generating enzymes cystathionine beta synthase and NOS would allow a regulated formation of HNO in neurons [2].…”

Section: Discussionmentioning

confidence: 93%

“…NO can be produced by the endothelial, the neuronal and the inducible isoform of the NO synthase, respectively eNOS, nNOS and iNOS [36]. The endothelial and inducible isoforms have been found in the dura mater, while their expression in the trigeminal ganglion and the STN is only marginal [3,37,38]. However, iNOS could have contributed to the endogenous NO generation in our cellular model, since the synthase can be induced by growth factors like NGF, which was added to the cell culture medium [39,40].…”

Section: Discussionmentioning

confidence: 99%

Hydrogen sulfide determines HNO-induced stimulation of trigeminal afferents

Wild

Meßlinger

Fischer

2015

Neuroscience Letters

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“…Digitally fixed images were analyzed using an image analyzer (Image Pro-Plus, Milan, Italy) by researchers unaware of the health condition of the mice and were calculated as percentage of positive area (%) according to Borsani and colleagues [ 41 , 42 ]. The analysis has been performed on five sections for each sample evaluating, for each section, six random fields with the same area (52 × 103 μm 2 ).…”

Section: Methodsmentioning

confidence: 99%

Involvement of Intestinal Goblet Cells and Changes in Sodium Glucose Transporters Expression: Possible Therapeutic Targets in Autistic BTBR T+Itpr3tf/J Mice

Franco

Bonomini

Borsani

et al. 2021

IJERPH

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Autism spectrum disorder is a neurodevelopmental syndrome with a complicated etiology and could be responsible for disrupted gastrointestinal tract microbiota. The aim of this work was to study intestinal samples from an autistic animal model (BTBR mouse strain) to better describe gastrointestinal alterations. We performed a morphological and biological evaluation of small intestine samples. In terms of morphology, we studied the goblet cells, cells of intestinal mucosal responsible for the production and maintenance of the protective mucous blanket. Alterations in their secretion may indicate an altered rate of mucus synthesis and this is one of the possible causes of gastrointestinal problems. In terms of biological evaluation, impaired regulation of glucose homeostasis regulated by sodium-glucose transporters has been suggested as an important component of obesity and associated comorbidities; therefore, this study analyzed the expression of sodium/glucose transporter-1 and -3 in BTBR mice to better define their role. We demonstrated that, in BTBR mice as compared to C57BL/6J (B6) strain animals: (1) The goblet cells had different protein content in their vesicles and apparently a larger number of Golgi cisternae; (2) the expression and level of sodium/glucose transporters were higher. These findings could suggest new possible targets in autism spectrum disorder to maintain mucus barrier function.

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Nitroxidergic system in human trigeminal ganglia neurons: A quantitative evaluation

Cited by 12 publications

References 44 publications

Single Administration of Melatonin Modulates the Nitroxidergic System at the Peripheral Level and Reduces Thermal Nociceptive Hypersensitivity in Neuropathic Rats

Single Administration of Melatonin Modulates the Nitroxidergic System at the Peripheral Level and Reduces Thermal Nociceptive Hypersensitivity in Neuropathic Rats

Hydrogen sulfide determines HNO-induced stimulation of trigeminal afferents

Involvement of Intestinal Goblet Cells and Changes in Sodium Glucose Transporters Expression: Possible Therapeutic Targets in Autistic BTBR T+Itpr3tf/J Mice

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