Nitrous oxide reductase

Pauleta, Sofia R.; Dell’Acqua, Simone; Moura, Isabel

doi:10.1016/j.ccr.2012.05.026

Cited by 152 publications

(116 citation statements)

References 170 publications

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“…Growth yield is a measure of metabolic efficiency (40), and higher growth yields indicate tight coupling between catabolism and energy conservation. A mechanistic understanding about the differences in electron transfer and energy conservation between clade I NosZ and clade II NosZ is lacking, and detailed structural studies are warranted to explain the observed growth yield differences (15,23,41,42). Organisms with more efficient energy conservation mechanisms can direct a greater fraction of electrons toward biomass synthesis (i.e., increased f s ), which provides a competitive advantage, particularly in oligotrophic environments.…”

Section: Discussionmentioning

confidence: 99%

Nitrous Oxide Reduction Kinetics Distinguish Bacteria Harboring Clade I NosZ from Those Harboring Clade II NosZ

Yoon

Nissen

Park

et al. 2016

Appl Environ Microbiol

146

153

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Nitrous oxide (N 2 O) is a potent greenhouse gas with a strong potential to drive climate change (1, 2) and will continue to be the largest contributor to ozone depletion in the stratosphere (2, 3). Anthropogenic activities, predominantly, fertilizer application in agricultural production, have contributed to a steady increase in atmospheric N 2 O concentrations, and a continued upward trend is expected (4-6). Particularly troublesome are the findings of a recent study that concluded that without solutions for the N 2 O problem, carbon dioxide (CO 2 ) emission reductions even greater than those already proposed will be required to avoid climate change (7). Due to its environmental impact, the pathways leading to the generation and consumption of N 2 O have received heightened interest.In the environment, N 2 O is predominantly formed as an intermediate of denitrification and a nitrification by-product (8). Denitrification is the stepwise reduction of NO 3 Ϫ /NO 2 Ϫ to gaseous products (i.e., N 2 O, N 2 ), with each step being mediated by distinct enzyme systems (9). A kinetic imbalance in the rates of reactions producing and consuming N 2 O during denitrification leads to the release of N 2 O to the atmosphere (8, 10). In nitrification, N 2 O is generated by nitrifier denitrification and as a byproduct of ammonia oxidation (8,11,12). A recent report indicated that nitrifiers, rather than denitrifiers, may be the primary source of N 2 O in agricultural soils (12). Other processes contributing to N 2 O formation include respiratory ammonification (also known as dissimilatory nitrate/nitrite reduction to ammonium [DNRA]) and chemodenitrification (i.e., the abiotic reaction of NO 2 Ϫ with ferrous iron) (13,14). In contrast to the diverse pathways of N 2 O generation, the only known major biological pathway for the removal of N 2 O is by reduction to N 2 , catalyzed by the enzyme nitrous oxide reductase (NosZ) (8,(15)(16)(17)

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Section: Discussionmentioning

confidence: 99%

Nitrous Oxide Reduction Kinetics Distinguish Bacteria Harboring Clade I NosZ from Those Harboring Clade II NosZ

Yoon

Nissen

Park

et al. 2016

Appl Environ Microbiol

146

153

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“…24,25) In addition, N 2 OR, encoded by α-subunit gene nosZ and other nos-related genes and located in the periplasm of Gram-negative bacteria, is the key enzyme in N 2 O emission. 26,27) Thus, incomplete denitrifiers that have lost the nosZ gene are active N 2 O emitters. Previous studies have shown that some Burkholderia spp.…”

mentioning

confidence: 99%

Nitrous oxide emission potentials of Burkholderia species isolated from the leaves of a boreal peat moss *Sphagnum fuscum**

Nie

Wang

et al. 2015

Bioscience, Biotechnology, and Biochemistry

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Using a culture-based nitrous oxide (N2O) emission assay, three active N2O emitters were isolated from Sphagnum fuscum leaves and all identified as members of Burkholderia. These isolates showed N2O emission in the medium supplemented with [Formula: see text] but not with [Formula: see text], and Burkholderia sp. SF-E2 showed the most efficient N2O emission (0.20 μg·vial(-1)·day(-1)) at 1.0 mM KNO3. In Burkholderia sp. SF-E2, the optimum pH for N2O production was 5.0, close to that of the phyllosphere of Sphagnum mosses, while the optimum temperature was uniquely over 30 °C. The stimulating effect of additional 1.5 mM sucrose on N2O emission was ignorable, but Burkholderia sp. SF-E2 upon exposure to 100 mg·L(-1) E-caffeic acid showed uniquely 67-fold higher N2O emission. All of the three N2O emitters were negative in both acetylene inhibition assay and PCR assay for nosZ-detection, suggesting that N2O reductase or the gene itself is missing in the N2O-emitting Burkholderia.

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“…1,2 Lessons can be taken from nature, where atmospheric N 2 O concentrations are regulated by the bacterial denitrification metalloenzyme, nitrous oxide reductase (N 2 OR). 3 The catalytic site in N 2 OR that is reactive under biological conditions is Cu Z *, 4 a [Cu 4 (μ 4 -S)] cluster characterized in the resting “1-hole” (3Cu I :1Cu II , S = ½) state 5,6 and active in the “fully reduced” (4Cu I , S = 0) state (Figure 1a). 7 Under certain conditions, the Cu Z * site in N 2 OR is replaced by Cu Z , 8 a [Cu 4 (μ 4 -S)(μ 2 -S)] cluster with a “2-hole” (2Cu I :2Cu II , S = 0) resting state that converts to a [Cu 4 (μ 4 -S)(μ 2 -SH)] cluster upon reduction to the 1-hole state, which shows relevant though limited N 2 O reductase activity (Figure 1b).…”

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confidence: 99%

A One-Hole Cu₄S Cluster with N₂O Reductase Activity: A Structural and Functional Model for Cu_Z*

et al. 2016

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During bacterial denitrification, two-electron reduction of N2O occurs at a [Cu4(μ4-S)] catalytic site (CuZ*) embedded within the nitrous oxide reductase (N2OR) enzyme. In this communication, an amidinate-supported [Cu4(μ4-S)] model cluster in its 1-hole (S = ½) redox state is thoroughly characterized. Along with its 2-hole redox partner and fully reduced clusters reported previously, the new species completes the two-electron redox series of [Cu4(μ4-S)] model complexes with catalytically relevant oxidation states for the first time. More importantly, N2O is reduced by the 1-hole cluster to produce N2 and the 2-hole cluster, thereby completing a closed cycle for N2O reduction. The title complex is thus not only the best structural model for CuZ* to date, but it also serves as a functional CuZ* mimic.

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Nitrous oxide reductase

Cited by 152 publications

References 170 publications

Nitrous Oxide Reduction Kinetics Distinguish Bacteria Harboring Clade I NosZ from Those Harboring Clade II NosZ

Nitrous Oxide Reduction Kinetics Distinguish Bacteria Harboring Clade I NosZ from Those Harboring Clade II NosZ

Nitrous oxide emission potentials of Burkholderia species isolated from the leaves of a boreal peat moss *Sphagnum fuscum**

A One-Hole Cu₄S Cluster with N₂O Reductase Activity: A Structural and Functional Model for Cu_Z*

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Nitrous oxide reductase

Cited by 152 publications

References 170 publications

Nitrous Oxide Reduction Kinetics Distinguish Bacteria Harboring Clade I NosZ from Those Harboring Clade II NosZ

Nitrous Oxide Reduction Kinetics Distinguish Bacteria Harboring Clade I NosZ from Those Harboring Clade II NosZ

Nitrous oxide emission potentials of Burkholderia species isolated from the leaves of a boreal peat moss Sphagnum fuscum*

A One-Hole Cu4S Cluster with N2O Reductase Activity: A Structural and Functional Model for CuZ*

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Nitrous oxide emission potentials of Burkholderia species isolated from the leaves of a boreal peat moss *Sphagnum fuscum**

A One-Hole Cu₄S Cluster with N₂O Reductase Activity: A Structural and Functional Model for Cu_Z*