Nitrosyl ethylenediaminetetraacetate ruthenium(II) complex promotes cellular growth and could be used as nitric oxide donor in plants

Osti, Renata Zachi de; Andrade, Julia Bolanho da Rosa; Souza, Juliana Pereira de; Silveira, Vanildo; Balbuena, Tiago Santana; Guerra, Miguel Pedro; Franco, Douglas Wagner; Floh, Eny Iochevet Segal; Santa–Catarina, Claudete

doi:10.1016/j.plantsci.2010.02.006

Cited by 9 publications

(12 citation statements)

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“…O. Ktze., an endangered conifer of economic importance native to Southern Brazil, have been shown to be an efficient system to study the effects of signaling molecules during somatic embryogenesis (Osti et al , dos Santos et al ). These cultures have been successfully employed to understand the effects of plant growth regulators, carbon sources and polyamines (PAs) on cellular growth, endogenous nitric oxide (NO) and PA metabolism, extracellular protein release and glucose and fructose uptake (Astarita and Guerra , Silveira et al , Steiner et al , Osti et al ).…”

Section: Introductionmentioning

confidence: 99%

Polyamines affect the cellular growth and structure of pro‐embryogenic masses in Araucaria angustifolia embryogenic cultures through the modulation of proton pump activities and endogenous levels of polyamines

Dutra

Silveira

Azevedo

et al. 2012

Physiologia Plantarum

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Polyamines (PAs) are abundant polycationic compounds involved in many physiological processes in plants, including somatic embryogenesis. This study investigates the role of PAs on cellular growth and structure of pro-embryogenic masses (PEMs), endogenous PA and proton pump activities in embryogenic suspension cultures of Araucaria angustifolia. The embryogenic suspension cultures were incubated with putrescine (Put), spermidine (Spd), spermine (Spm) and the inhibitor methylglyoxal-bis(guanylhydrazone) (MGBG), respectively (1 mM). After 24 h and 21 days, the cellular growth and structure of PEMs, endogenous PA contents and proton pump activities were analyzed. The addition of Spm reduced the cellular growth and promoted the development of PEMs in embryogenic cultures, which could be associated with a reduction in the activities of proton pumps, such as H(+) -ATPase P- and V-types and H(+) -PPases, and alterations in the endogenous PA contents. Spm significantly affected the physiology of the A. angustifolia somatic embryogenesis suspension, as it potentially affects cellular growth and structure of PEMs through the modulation of proton pump activities. This work demonstrates the involvement of exogenous PAs in the modulation of cellular growth and structure of PEMs, endogenous PA levels and proton pump activities during somatic embryogenesis. To our knowledge, this study is the first to report a relationship between PAs and proton pump activities in these processes. The results obtained in this study offer new perspectives for studies addressing the role of PAs and proton pump on somatic embryogenesis in this species.

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Section: Introductionmentioning

confidence: 99%

Polyamines affect the cellular growth and structure of pro‐embryogenic masses in Araucaria angustifolia embryogenic cultures through the modulation of proton pump activities and endogenous levels of polyamines

Dutra

Silveira

Azevedo

et al. 2012

Physiologia Plantarum

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“…Além disso, sugeriram uma possível correlação entre a produção de NO, induzida ou não pelas PAs, e a aquisição de competência para a embriogênese. O efeito da aplicação exógena de um doador de NO foi avaliado por Osti et al (2010) também neste sistema, onde verificaram que a adição de NO interfere no crescimento celular, dentro de um limite de concentração, quando suplementado ao meio de cultura. Jo et al (2013) sugeriram que o potencial embriogênico das CEs de A.…”

Section: Embriogênese Zigótica E Somática Em Coníferasunclassified

“…O aumento dos níveis de ERO causado pelo estresse oxidativo,em decorrência da adição de diferentes promotores de maturação (ABA e agentes osmóticos) nas culturas in vitro,pode promover a ES em diversas espécies de plantas (Caliskan et al, 2004;Ganesan & Jayabalan 2004;Luo et al, 2001;Pasternak et al, 2002;Andrade, 2010). Durante a indução da ES, as culturas celulares apresentam um processo rápido de proliferação celular e metabolismo aeróbico ativo, promovendo o acúmulo de ERO em células embriogênicas (Stasolla et al, 2004).…”

Section: O óXido Nítrico (No) Espécies Reativas De Oxigênio (Ero) E unclassified

“…angustifolia, a proliferação das células foi afetada quando da incorporação de diferentes concentrações de NO (Osti et al, 2010;Andrade 2010). Andrade (2010),propôs que, neste sistema vegetal os agentes promotores de maturação reduzem a síntese endógena de NO e ERO, e que o bloqueio dos estímulos necessários para a formação do embrião ocorre após a sinalização de NO.…”

Section: O óXido Nítrico (No) Espécies Reativas De Oxigênio (Ero) E unclassified

“…O NO é um radical livre gasoso, com baixo peso molecular (Corpas et al, 2006), sua sinalização inclui diversos processos fisiológicos como diferenciação celular, morte celular programada, embriogênese, germinação, crescimento de raiz e resposta a estresse (Silveira et al, 2006;Osti et al, 2010;Beligni et al, 2002;Santa-Catarina, et al, 2007). O NO é sintetizado, principalmente em tecidos com crescimento ativo, como o eixo embrionário e cotilédones, com níveis menores nos tecidos maduros e órgãos senescentes (Leshem et al, 1996;Caro & Puntarulo, 1999).…”

Section: Efeito Do As Gsno E Ptio Na Produção De No E Ero Em Suspensunclassified

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Ácido salicílico como sinalizador durante a embriogênese de Araucaria angustifolia (Bert.) O. Kuntze

Bueno¹

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Agradeço a todos que conviveram comigo durante o período de realização do mestrado, tornando possível a realização deste trabalho. Aos meus pais, Reinaldo e Leopoldo, minhas mães Claudia e Lourdes, aos meus irmãos Raphael e Arthur e minhas irmãs Camila e Flávia, por todo o apoio, incentivo e cuidado. Ao meu querido marido Daniel Juan por ser uma pessoa maravilhosa. À profa. Eny Floh por me receber e oferecer a oportunidade de realizar um mestrado, por toda a orientação, confiança e paciência desde meu ingresso no BIOCEL. Aos integrantes do BIOCEL durante o projeto, Carmem Silva, Leonardo

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Nitric oxide elicitation for secondary metabolite production in cultured plant cells

Zhang

Wang

2011

Appl Microbiol Biotechnol

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Nitric oxide (NO) is an important signal molecule in stress responses. Accumulation of secondary metabolites often occurs in plants subjected to stresses including various elicitors or signal molecules. NO has been reported to play important roles in elicitor-induced secondary metabolite production in tissue and cell cultures of medicinal plants. Better understanding of NO role in the biosynthesis of such metabolites is very important for optimizing the commercial production of those pharmaceutically significant secondary metabolites. This paper summarizes progress made on several aspects of NO signal leading to the production of plant secondary metabolites, including various abiotic and biotic elicitors that induce NO production, elicitor-triggered NO generation cascades, the impact of NO on growth development and programmed cell death in medicinal plants, and NO-mediated regulation of the biosynthetic pathways of such metabolites. Cross-talks among NO signaling and reactive oxygen species, salicylic acid, and jasmonic acid are discussed. Some perspectives on the application of NO donors for induction of the secondary metabolite accumulation in plant cultures are also presented.

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Nitrosyl ethylenediaminetetraacetate ruthenium(II) complex promotes cellular growth and could be used as nitric oxide donor in plants

Cited by 9 publications

References 41 publications

Polyamines affect the cellular growth and structure of pro‐embryogenic masses in Araucaria angustifolia embryogenic cultures through the modulation of proton pump activities and endogenous levels of polyamines

Polyamines affect the cellular growth and structure of pro‐embryogenic masses in Araucaria angustifolia embryogenic cultures through the modulation of proton pump activities and endogenous levels of polyamines

Ácido salicílico como sinalizador durante a embriogênese de Araucaria angustifolia (Bert.) O. Kuntze

Nitric oxide elicitation for secondary metabolite production in cultured plant cells

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