“…A great number of searches regarding the vegetative improvement of this species have been performed in the last years, mostly taking the vegetative propagation as the main technic for this objective. However, there are still many challenges regarding this technic, mainly aiming to overcome the ontogenetic age (WENDLING et al, 2013;STUEPP et al, 2015;2017a, 2017bNASCIMENTO et al, 2018) as well as regarding the nutrition of the mother tree (NASCIMENTO et al, 2019), the use of growth regulators (BITENCOURT et al, 2009;NAGAOKA et al, 2013;PIMENTEL et al, 2019;NASCIMENTO et al, 2020), testing established clones (BRONDANI et al, 2008;2009;BISOGNIN et al, 2018;PIMENTEL et al, 2019;GAZZANA et al, 2020;VIEIRA et al, 2021) and rooting environments (BRONDANI et al, 2008;2009;NASCIMENTO et al, 2020). In this manner, it is important to take in consideration the possibility of different vegetative propagation potentials of I. paraguariensis from contrasting natural populations, whereas specific conditions such as maturation, for example, may not be a determinant factor.…”