Nitrogenase activity in the rhizosphere of Stachys sylvatica and some other dicotyledenous plants

Harris, D.; Dart, P. J.

doi:10.1016/0038-0717(73)90013-8

Cited by 24 publications

(5 citation statements)

References 4 publications

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“…There are two factors in particular which may have infiuenced the levels of activity measured in our investigations. Recent reports indicate that the main site of activity may be in the rhizosphere of living roots (Dobereiner et al, 1972;Harris and Dart, 1973), and the absence of a large number of living roots from most of our samples may account for the low activities that were measured. Also, the partial pressure of oxygen in the gas phase of our aerobic incubations was Non-symbiotic nitrogen fixation 11 20 kPa (0-2 atm) whereas activity may be higher at 4 kPa (004 atm) (Dobereiner et al, 1972).…”

Section: Discussionmentioning

confidence: 85%

Non‐symbiotic nitrogen fixation in some soils of England and Wales

Lockyer

Cowling

1977

Grass and Forage Science

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The acetylene-reduction method was used to estimate the fixation of atmospheric nitrogen by non-symbiotic micro-organisms in soils from England and Wales. Significant fixation was found only in the surface horizon of the soil (0-1 cm) and was much higher in the light than in the dark. The highest rates were found with samples having a well-developed surface film of blue-green algae and were from 6 to 10 g ha"' N per h. Generally, rates were very much lower. The addition of fertilizer nitrogen at 100 kg ha"' markedly reduced N fixation by blue-green algae.

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Section: Discussionmentioning

confidence: 85%

Non‐symbiotic nitrogen fixation in some soils of England and Wales

Lockyer

Cowling

1977

Grass and Forage Science

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“…The reduction of acetylene to ethylene specifii-cally was proposed as an indirect method to assay for nitrogenase and has been used widely (94,97,98). Acetylene reduction by grasses has been measured by using in situ assays (8,197), soil cores removed from the field (46,197), pots containing greenhouse-grown plants (99,100), and excised roots (1,47,64,208). The soil surrounding the roots is usually included in the assay chamber when the in situ, intact soil core, and flower pot methods are used.…”

Section: Methodology and Problems Associated With Measurement Of Nitrmentioning

confidence: 99%

“…In some cases roots of grasses have been shown to support acetylene reduction, and nitrogen-fixing bacteria have been cultured from these samples (13,53,64,72,99,208,209). However, the isolation of nitrogen-fixing bacteria in pure culture does not prove that an isolate fixes nitrogen or is solely responsible for nitrogenase activity in grass roots.…”

Section: Physiology Of Nitrogen-fixingmentioning

confidence: 99%

Evaluation of nitrogen fixation by bacteria in association with roots of tropical grasses.

Berkum¹,

Bohlool²

1980

Microbiological Reviews

121

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“…In situ assays have also been used but are cumbersome and the measurements are difficult to interpret (Balandreau and Dommergues 1973;Tjepkema and van Berkum 1977). Greenhouse-grown, potted plants (Harris and Dart 1973;Hirota et ai. 1978) have also been assayed for nitrogenase activity, but lSubmitted as Conference paper No.…”

Section: Introductionmentioning

confidence: 99%

Factors affecting nitrogenase activity (C₂H₂ reduction) associated with sorghum and millet estimated using the soil core assay

Wani¹,

Dart²,

Upadhyaya³

1983

Can. J. Microbiol.

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Factors affecting nitrogenase activity associated with sorghum and millet roots have been studied. Plants grown in iron cores in the field and then assayed had significantly higher activity than plants cored at the time of assay. Mechanical disturbance during transportation of the cores reduced the activity significantly. Any delay between cutting off the plant top and injecting C2H2 gas led to a reduction in the level of nitrogenase activity determined. Diurnal variation in nitrogenase activity was noted but was not correlated with soil temperature. Most activity occurred at the end of the photoperiod. Seasonal variation in nitrogenase activity of plants was observed and was correlated with the ontogenetic development of the host plant, being most at flowering. A low but significant correlation existed between soil moisture content and nitrogenase activity associated with the plant.

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Nitrogenase activity in the rhizosphere of Stachys sylvatica and some other dicotyledenous plants

Cited by 24 publications

References 4 publications

Non‐symbiotic nitrogen fixation in some soils of England and Wales

Non‐symbiotic nitrogen fixation in some soils of England and Wales

Evaluation of nitrogen fixation by bacteria in association with roots of tropical grasses.

Factors affecting nitrogenase activity (C₂H₂ reduction) associated with sorghum and millet estimated using the soil core assay

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Nitrogenase activity in the rhizosphere of Stachys sylvatica and some other dicotyledenous plants

Cited by 24 publications

References 4 publications

Non‐symbiotic nitrogen fixation in some soils of England and Wales

Non‐symbiotic nitrogen fixation in some soils of England and Wales

Evaluation of nitrogen fixation by bacteria in association with roots of tropical grasses.

Factors affecting nitrogenase activity (C2H2 reduction) associated with sorghum and millet estimated using the soil core assay

Contact Info

Product

Resources

About

Factors affecting nitrogenase activity (C₂H₂ reduction) associated with sorghum and millet estimated using the soil core assay