Nitrogen transfer in the arbuscular mycorrhizal symbiosis

Govindarajulu, Manjula; Pfeffer, Philip E.; Hai-ru, Jin; Abubaker, Jehad; Douds, David D.; Allen, James W.; Bücking, Heike; Lammers, Peter J.; Shachar‐Hill, Yair

doi:10.1038/nature03610

Cited by 880 publications

(679 citation statements)

References 28 publications

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“…Several studies revealed that approximately 20 to 75% of the total N uptake of AM plants can be transferred by AMF to their hosts (Govindarajulu et al 2005;Tanaka and Yano 2005). An observed wide range of mycorrhizal growth responses of rice plants in response to nutrient availability, however, impedes understanding of the molecular mechanisms underlying this N transfer.…”

Section: Discussionmentioning

confidence: 99%

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Identification of arbuscular mycorrhiza-inducible Nitrate Transporter 1/Peptide Transporter Family (NPF) genes in rice

Drechsler¹,

Courty²,

Brulé³

et al. 2017

Mycorrhiza

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Arbuscular mycorrhizal fungi (AMF) colonize up to 90% of all land plants and facilitate the acquisition of mineral nutrients by their hosts. Inorganic orthophosphate (P i ) and nitrogen (N) are the major nutrients transferred from the fungi to plants. While plant P i transporters involved in nutrient transfer at the plant-fungal interface have been well studied, the plant N transporters participating in this process are largely unknown except for some ammonium transporters (AMT) specifically assigned to arbuscule-colonized cortical cells. In plants, many nitrate transporter 1/peptide transporter family (NPF) members are involved in the translocation of nitrogenous compounds including nitrate, amino acids, peptides and plant hormones. Whether NPF members respond to AMF colonization, however, is not yet known. Here, we investigated the transcriptional regulation of 82 rice (Oryza sativa) NPF genes in response to colonization by the AMF Rhizophagus irregularis in roots of plants grown under five different nutrition regimes. Expression of the four OsNPF genes NPF2.2/ PTR2, NPF1.3, NPF6.4 and NPF4.12 was strongly induced in mycorrhizal roots and depended on the composition of the fertilizer solution, nominating them as interesting candidates for nutrient signaling and exchange processes at the plantfungal interface.

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Section: Discussionmentioning

confidence: 99%

“…Govindarajulu et al 2005;Koegel et al 2013a,b; reviewed by Courty et al 2015). Due to high energy costs of NO 3 − to NH 4 + reduction, microorganisms normally prefer the direct assimilation of NH 4 + (Courty et al 2015).…”

Section: Introductionmentioning

confidence: 99%

Identification of arbuscular mycorrhiza-inducible Nitrate Transporter 1/Peptide Transporter Family (NPF) genes in rice

Drechsler¹,

Courty²,

Brulé³

et al. 2017

Mycorrhiza

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“…GzRPS16 has been annotated as a mitochondrial ribosomal protein S16 that characterized as a human housekeeping gene (Eisenberg and Levanon, 2003). In addition, ribosomal protein S4 has been used as a reference gene for qRT-PCR in a mycorrhizal fungus (Govindarajulu et al, 2005). RPS3 and RPS18 were the most stable in a fungus-infected red flour beetle (Lord et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Potential Reference Genes for Quantitative RT-PCR Analysis in Fusarium graminearum under Different Culture Conditions

Kim¹,

Yun²

2011

The Plant Pathology Journal

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The filamentous fungus Fusarium graminearum is an important cereal pathogen. Although quantitative realtime PCR (qRT-PCR) is commonly used to analyze the expression of important fungal genes, no detailed validation of reference genes for the normalization of qRT-PCR data has been performed in this fungus. Here, we evaluated 15 candidate genes as references, including those previously described as housekeeping genes and those selected from the whole transcriptome sequencing data. By a combination of three statistical algorithms (BestKeeper, geNorm, and NormFinder), the variation in the expression of these genes was assessed under different culture conditions that favored mycelial growth, sexual development, and trichothecene mycotoxin production. When favoring mycelial growth, GzFLO and GzUBH expression were most stable in complete medium. Both EF1A and GzRPS16 expression were relatively stable under all conditions on carrot agar, including mycelial growth and the subsequent perithecial induction stage. These two genes were also most stable during trichothecene production. For the combined data set, GzUBH and EF1A were selected as the most stable. Thus, these genes are suitable reference genes for accurate normalization of qRT-PCR data for gene expression analyses of F. graminearum and other related fungi.

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“…Additionally, legumes can be inoculated with selected symbionts to improve survival in reforestation programs [3,33]. Since Fabaceae can support rhizobia and both EM and AM, the latter being the most frequent [70], plant performance could be favored by nitrogen (N) uptake, either by direct uptake by AM [71] or by the multiple benefits from AM [72].…”

Section: Plants For Reforestationmentioning

confidence: 99%

Mycorrhizal Interactions for Reforestation: Constraints to Dryland Agroforest in Brazil

Pagano

Cabello

2011

ISRN Ecology

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Reforestation provides restoration of forest ecosystem services including improved soil fertility, which leads to increased productivity and/or sustainability of the system. Trees also increase the average carbon stocks providing wood supply for local communities; however, C sequestration strategies highlight tree plantations without considering their full environmental consequences, such as losses in stream flow. The productivity of a site is a consequence of their physical, chemical, and biological properties, resulting in natural fertile soils or adequate managed soils for improved quality. Thus, it is required to know the variations in the properties of land-use systems for adoptability of agroforestry innovations. The choice of agroforestry tree species (highly mycorrhizal dependent plants should be selected) would have great implications for the manipulation of arbuscular mycorrhizas's species. In dry forest, the inevitable consequence of cutting has been the loss of vegetation cover and insufficient scientific information on the capacity to optimize forest recuperation affects agroforestry adoption. To study the biological properties of soils is now of interest; therefore, this paper reviews the literature that has hitherto been published on mycorrhizal interactions for reforestation and points out the use of mycorrhizal technology as one of the alternatives to improve forest products and environmental quality.

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Nitrogen transfer in the arbuscular mycorrhizal symbiosis

Cited by 880 publications

References 28 publications

Identification of arbuscular mycorrhiza-inducible Nitrate Transporter 1/Peptide Transporter Family (NPF) genes in rice

Identification of arbuscular mycorrhiza-inducible Nitrate Transporter 1/Peptide Transporter Family (NPF) genes in rice

Evaluation of Potential Reference Genes for Quantitative RT-PCR Analysis in Fusarium graminearum under Different Culture Conditions

Mycorrhizal Interactions for Reforestation: Constraints to Dryland Agroforest in Brazil

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