Nitrogen-to-protein conversion factor for ten cereals and six legumes or oilseeds. A reappraisal of its definition and determination. Variation according to species and to seed protein content

Mossé, Jacques

doi:10.1021/jf00091a004

Cited by 332 publications

(307 citation statements)

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“…4). Grain Zn and Fe concentrations and grain protein content of the Figure 4 Relationship between Zn (k) and Fe () concentrations and protein content in the grains, determined using a N:protein conversion factor (Mosse 1990). grains were positively correlated (R 2 00.78 and 0.91, respectively Fig.…”

Section: Grain Protein Contentsmentioning

confidence: 99%

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Hydrolysed wool: a novel soil amendment for zinc and iron biofortification of wheat

Gogos

Evangelou

Schäffer

et al. 2013

New Zealand Journal of Agricultural Research

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Biofortification*with either the application of zinc and iron fertilizer or the application of amendments to increase their bioavailability in soil*is a possible strategy to tackle worldwide micronutrient malnutrition. We investigated the effect of hydrolysed wool on the uptake of zinc and iron by wheat (Triticum aestivum var. Greina). We performed pot experiments in which either hydrolysed wool or mineral fertilizer of the same elemental composition was incorporated into a loamy-sand collected from an agricultural field. Zinc grain concentrations were 37.7 mg kg(1 (control), 45.5 mg kg (1 (mineral fertilization) and 54.1 mg kg(1 (hydrolysed wool). In addition, hydrolysed wool application increased grain yield 2-fold and grain protein content 1.5-fold, compared with 1.4-fold and 1.3-fold, respectively, by the mineral fertilizer. We propose that hydrolysed wool could be used to supplement other fertilizers, enhancing the latter with an easily available N source as well as promoting zinc and iron uptake in plants.

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Section: Grain Protein Contentsmentioning

confidence: 99%

“…The total nitrogen content of the grain samples was determined after dry combustion using a CNS-2000 Analyzer (Leco, US-Saint Joseph, Michigan). The grain protein contents were determined using the N:protein conversion factors given by Mosse (1990).…”

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confidence: 99%

Hydrolysed wool: a novel soil amendment for zinc and iron biofortification of wheat

Gogos

Evangelou

Schäffer

et al. 2013

New Zealand Journal of Agricultural Research

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“…protein concentration is obtained by multiplying its total N by a nitrogen to protein conversion factor which is calculated from the amino acids composition (Mosse, 1990). -11.26(0.835) In this study the method to determine total N was persulphate digestion, which was developed as an alternative to the Kjeldahl method (Delia et al, 1977).…”

Section: Materials and Methods 124mentioning

confidence: 99%

“…When converting total N content of WBF to total protein, two Nitrogen Factor (NF) recommended in previous studies were compared -5.7, a recommended NF for wheat grain (Mosse, 1990, Barikmo et al, 2003) and 5.52, a proposed NF for wheat flour (Mariotti et al, 2008, Sarwar et al, 1983. In this research, NF 5.52 was applied to WBF due to wheat flour being the main component of WBF.…”

Section: Structural Carbohydrates and Lignin Content (% Of Dry Weight)mentioning

confidence: 99%

Life Cycle Assessment (LCA) of Light-Weight Eco-composites

Guo¹

2012

Springer Theses

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LCA of Light-weight Eco-composites Statement of originality 2 Statement of originalityI hereby declare that this submission is my own work and to the best of my knowledge it contains no materials previously published or written by another person.Contributions made to the research of this thesis by others, with whom I have worked with are explicitly acknowledged. I also declare that the intellectual content of this thesis is the product of my own work, except to the extent that assistance from others with data collection and modification of process-oriented model DNDC is acknowledged. Miao GuoSep 2010 LCA of Light-weight Eco-compositesAbstract 3 AbstractThe environmental profiles of novel wheat based foam materials were investigated in this thesis using Life Cycle Assessment (LCA) methods. The LCAs were developed using primary data collected from industrial sources combined with new laboratory experiments supplemented with secondary data from publicly available sources.Laboratory research was conducted to obtain important missing data on WBFs for the LCA modelling, including physico-chemical parameters, biodegradability and energy recovery under anaerobic digestion conditions.Contribution analysis suggested that the emissions evolved from the wheat agroecosystem and PVOH production, together with the energy and infrastructure involved in WBF production were the major contributors to the environmental burdens of the WBF life cycle in most impact categories. The atmospheric emissions resulting from WBF degradation at the end-of-life also emerged as another important contributor to environmental impact. Amongst the diverse ‗end-of-life' scenarios examined, AD and home composting were suggested to be the optimum choices for WBF waste treatment. This research discusses two N 2 O modelling approaches and presents a method to expand the system boundary by integrating the process-oriented model DNDC for field emissions into the LCA. Sensitivity analysis suggests that the environmental profiles of agricultural products are influenced substantially by the system boundary definition. LCA of Light-weight Eco-compositesAbstract 4 Furthermore, it suggests that the ‗general rule' in LCA practice by applying an empirical model or a default emission factor (EF) could deliver unreliable LCA findings.This study also evaluated the sensitivity of the LCA results to methodology and data variations and quantified the uncertainties in the LCA outcomes arising from uncertainty in the inventory and data variability. This has led to an increase in confidence in the LCA findings. At the same time it indicates the areas where improvements in data or methods are needed in order for robust conclusions to be drawn and unbiased information to be delivered e.g. the methodological rigidity of characterization models, the IPCC Tier 1 EF uncertainty range. LCA of Light-weight Eco-compositesAcknowledgements 5

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“…Nitrogen was determined by the semi-micro Kjeldahl method (Cunnif, 1995) and protein as crude protein (N x 5.4) (Mossé, 1990). Nitrogen 15 was determined in the whole flour and protein fractions from the titres obtained in the nitrogen determinations (Bremner, 1965;International Atomic Energy Agency, 1972).…”

Section: Methodsmentioning

confidence: 99%

Nitrogen 15 abundance in protein fractions of beans fertilized with (15NH4)2SO4

Chaud

Oliveira

2002

Sci. agric. (Piracicaba, Braz.)

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Studies evaluating the protein nutritive value of beans labelled with 15 N, using nitrogen balance and the quantitation of faecal and urinary endogenous nitrogen, determined by isotopic dilution, have been extensively used. The objective of this research was to verify if the isotopic labelling of raw, freeze dried beans (Phaseolus vulgaris L., cultivar Piratã 1) with 1.394 atoms% 15 N, resulted in the same abundance of the whole flour and of the protein fractions extracted from the beans with 0.5 mol L -1 NaCl. The isotopic abundance found in the whole bean flour, in the protein extract, in the globulin and albumin fractions were respectively: 1.394 ± 0.011; 1.403 ± 0.012; 1.399 ± 0.007 and 1.399 ± 0.028 atoms % of 15 N, presenting no difference (P > 0.05). However, a difference was found (P < 0.05) between the above mentioned abundances and the isotopic abundance found in the nitrogen of the proteins in the extraction residue, which was 0.969 ± 0.084. Since the abundances did not differ, the protein nutritive indexes, such as digestibility and biological value, determined from the nitrogen balance and corrected for isotopic dilution, would not be affected by extracting the proteins from the beans with 0.5 mol L -1 NaCl. If working with the nitrogen balance of the residual proteins after extraction and even with the whole flours, these indexes could present incorrect values, since the isotopic labelling of the residual proteins was less than that of the protein fractions. RESUMO: Estudos para determinação do valor nutritivo da proteína de feijões marcados com 15 N, utilizando balanço de nitrogênio e quantificação de nitrogênio endógeno fecal e urinário, determinados por diluição isotópica, têm sido muito utilizados. O objetivo deste trabalho foi verificar se a abundância de 15 N feijão cru e liofilizado (Phaseolus vulgaris L., cultivar Piratã 1), de 1,394 átomos % de 15 N, era a mesma, na farinha integral do feijão, no extrato protéico e nas frações protéicas extraídas do feijão com NaCl 0,5 mol L -1 . As abundâncias encontradas na farinha integral, extrato protéico e frações protéicas globulina e albumina foram, respectivamente: 1.394 ± 0.011; 1.403 ± 0.012; 1.399 ± 0.007 e 1.399 ± 0.028 átomos % de 15 N, não apresentando diferença (P > 0,05). Entretanto, foi encontrada diferença (P < 0,05) entre as referidas abundâncias e aquela encontrada nas proteínas que permaneceram no resíduo da extração, que foi 0,969 ± 0,084 átomos de 15 N. Uma vez que as marcações isotópicas não diferiram, pôde-se inferir que os índices de valor nutritivo de proteínas, como a digestibilidade ou valor biológico, determinados por balanço de nitrogênio e corrigidos por diluição isotópica, não serão afetados pela extração das proteínas do feijão com NaCl 0,5 mol L -1 . No caso das proteínas residuais ou mesmo de farinhas integrais, estes índices podem apresentar valores incorretos, visto que a abundância de 15 N das proteínas residuais foi menor. Palavras-chave: Phaseolus vulgaris L., isótopos estáveis, marcação isotópica, valor ...

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Nitrogen-to-protein conversion factor for ten cereals and six legumes or oilseeds. A reappraisal of its definition and determination. Variation according to species and to seed protein content

Cited by 332 publications

References 18 publications

Hydrolysed wool: a novel soil amendment for zinc and iron biofortification of wheat

Hydrolysed wool: a novel soil amendment for zinc and iron biofortification of wheat

Life Cycle Assessment (LCA) of Light-Weight Eco-composites

Nitrogen 15 abundance in protein fractions of beans fertilized with (15NH4)2SO4

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