Nitrogen Starvation-induced Phosphorylation of Ras1 Protein and Its Potential Role in Nutrient Signaling and Stress Response

Jin, Xin; Starke, Samuel J.; Li, Yang; Sethupathi, Sheetal; Kung, George; Dodhiawala, Paarth B.; Wang, Yuqi

doi:10.1074/jbc.m115.713206

Cited by 9 publications

(8 citation statements)

References 38 publications

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“…We can see that in the 3 D exposure period, with the increase of exposure days, the inhibition rate showed first increased and then decreased PRP on the growth of Chlorella, exposed 1 peaked at d after growth inhibition rate began to decrease, showed the strongest inhibitory effect of PRP on the initial growth of chlorella growth after inhibition will be weakened. The reasons are: PRP effect of the short duration of exposure at 1 d after Chlorella resistance, which leads to growth inhibition rate declined, the situation in the research of other scholars also had similar reports 22,23 . At the same time, with the change of PRP concentration, PRP on chlorella growth inhibition rate showed obvious dose effect relationship, with the increase in the concentration of ANT exposure, chlorella growth inhibition rate increased.…”

Section: Exposure To Different Concentrations Of Prp Inhibitory Effecsupporting

confidence: 55%

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Variations of the toxic potential of drinking water containing propyphenazone before and after chlorine disinfection

2017

JECET

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Quinolones -typical pyrazole in drinking water are often exposed to the Isopropylphenazone (PRP) as the research object, investigated with variation of acute toxicity of water and the genetic toxicity of PRP before and after chlorination of drinking water. The results of acute toxicity test showed that Chlorella, different concentrations of PRP (0, 0.004, 0.009, 0.017, 0.035, 0.069, 0.139 mmol/L) exposure conditions, the Chlorella growth inhibition rate showed obvious dose effect relationship, with the increase in the concentration of PRP exposure, chlorella growth inhibition rate increased in the high dosage of chlorine; (the concentration ratio of PRP and 1:2 and 1:5 Moore chloride), inhibition on the growth of Chlorella water after chlorination rate was higher than that in low chloride before disinfection, chlorine dosage (concentration ratio of PRP and 4:1 and 1:1 Moore chloride), water samples of chlorella growth inhibition rate was below the chlorination, show low chlorine dosage can reduce the risk of acute toxicity of water produced by PRP. The results of genetic toxicity test showed that the SOS/umu, both with and without exogenous metabolic enzymes S9, PRP showed a good dose-response relationship, the induction ratio (IR value) increases with the increasing of PRP concentration, but overall genetic toxicity is relatively small (IR<1.5); PRP in the chlorination process. The IR value increased with the increase of the amount of chlorine and gradually become larger, direct and indirect genotoxicity toxicity equivalent of TEQ4-NQO and TEQBaP etc. were increased, showed that the direct genetic toxicity of PRP containing drinking water

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Section: Exposure To Different Concentrations Of Prp Inhibitory Effecsupporting

confidence: 55%

“…The chlorella growth inhibition rate 23 is below 40% , that there are great differences between the different types of pyrazolone drugs on chlorella growth inhibition, acute toxic effects of PRP on Chlorella vulgaris was higher than that of AMP.…”

Section: Fig1:the Growth Inhibition Percentages Of Chlorella Vulgarimentioning

confidence: 99%

Variations of the toxic potential of drinking water containing propyphenazone before and after chlorine disinfection

2017

JECET

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“…Next we sought to identify the kinase that is responsible for Gpa2 phosphorylation. One candidate is glycogen synthase kinase (GSK), 2 because GSK is known to be active under nitrogen starvation conditions, and there are predicted GSK phosphorylation sites on Gpa2 (24,25). There are a total of four GSK isoforms in yeast: Mrk1, Mck1, Rim11, and Ygk3 (YOL128C).…”

Section: Glycogen Synthase Kinases Are Required For Gpa2 Phosphorylationmentioning

confidence: 99%

Phosphorylation of the Gα protein Gpa2 promotes protein kinase A signaling in yeast

Huang

Benben

Green

et al. 2019

Journal of Biological Chemistry

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“…S. cerevisiae Ras2 regulates nutrient signaling (23) and glucose activation of cAMP synthesis (24). However, in C. albicans, the Ras1-cAMP pathway plays a broader role in the response to a range of environmental stimuli, including temperature, serum, and pH, to induce filamentation (25,26).…”

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confidence: 99%

Sho1p Connects Glycolysis to Ras1-cAMP Signaling and Is Required for Microcolony Formation in Candida albicans

Kumar

Maulik

Pathirana

et al. 2020

mSphere

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ABSTRACT Candida albicans is an opportunistic, dimorphic fungus that causes candidiasis in immunocompromised people. C. albicans forms specialized structures called microcolonies that are important for surface adhesion and virulence. Microcolonies form in response to specific environmental conditions and require glycolytic substrates for optimal growth. However, fungal signaling pathways involved in sensing and transmitting these environmental cues to induce microcolony formation have not been identified. Here, we show that the C. albicans Ras1-cAMP cascade is required for microcolony formation, while the Cek1-MAP kinase pathway is not required, and Hog1 represses microcolony formation. The membrane protein Sho1, known to regulate the Cek1 pathway in yeasts, was indispensable for C. albicans microcolony formation but regulated the Ras1-cAMP pathway instead, based upon diminished intracellular levels of cAMP and reduced expression of core microcolony genes, including HWP1, PGA10, and ECE1, in C. albicans sho1Δ cells. Based upon predicted physical interactions between Sho1 and the glycolytic enzymes Pfk1, Fba1, Pgk1, and Cdc19, we hypothesized that Sho1 regulates Ras1-cAMP by establishing cellular energy levels produced by glycolysis. Indeed, microcolony formation was restored in C. albicans sho1Δ cells by addition of exogenous intermediates of glycolysis, including downstream products of each predicted interacting enzyme (fructose 1,6 bisphosphate, glyceraldehyde phosphate, 3-phosphoglyceric acid, and pyruvate). Thus, C. albicans Sho1 is an upstream regulator of the Ras1-cAMP signaling pathway that connects glycolytic metabolism to the formation of pathogenic microcolonies. IMPORTANCE C. albicans microcolonies form extensive hyphal structures that enhance surface adherence and penetrate underlying tissues to promote fungal infections. This study examined the environmental conditions that promote microcolony formation and how these signals are relayed, in order to disrupt signaling and reduce pathogenesis. We found that a membrane-localized protein, Sho1, is an upstream regulator of glycolysis and required for Ras1-cAMP signaling. Sho1 controlled the Ras1-dependent expression of core microcolony genes involved in adhesion and virulence. This new regulatory function for Sho1 linking glycolysis to microcolony formation reveals a novel role for this fungal membrane protein.

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Nitrogen Starvation-induced Phosphorylation of Ras1 Protein and Its Potential Role in Nutrient Signaling and Stress Response

Cited by 9 publications

References 38 publications

Variations of the toxic potential of drinking water containing propyphenazone before and after chlorine disinfection

Variations of the toxic potential of drinking water containing propyphenazone before and after chlorine disinfection

Phosphorylation of the Gα protein Gpa2 promotes protein kinase A signaling in yeast

Sho1p Connects Glycolysis to Ras1-cAMP Signaling and Is Required for Microcolony Formation in Candida albicans

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