Nitrogen Sources and Iron Availability Affect Pigment Biosynthesis and Nutrient Consumption in Anabaena sp. UTEX 2576

Abstract

Anabaena sp. UTEX 2576 metabolizes multiple nitrogen (N) sources and is deemed a biotechnological platform for chemical production. Cyanobacteria have been identified as prolific producers of biofertilizers, biopolymers, biofuels, and other bioactive compounds. Here, we analyze the effect of different N-sources and Fe availability on the bioproduction of phycobiliproteins and β-carotene. We characterize nutrient demand in modified BG11 media, including data on CO2 fixation rates, N-source consumption, and mineral utilization (e.g., phosphorus (P), and 11 metallic elements). Results suggest that non-diazotrophic cultures grow up to 60% faster than diazotrophic cells, resulting in 20% higher CO2-fixation rates. While the production of β-carotene was maximum in medium with NaNO3, Fe starvation increased the cellular abundance of C-phycocyanin and allophycocyanin by at least 22%. Compared to cells metabolizing NaNO3 and N2, cultures adapted to urea media increased their P, calcium and manganese demands by at least 72%, 97% and 76%, respectively. Variations on pigmentation and nutrient uptake were attributed to changes in phycocyanobilin biosynthesis, light-induced oxidation of carotenoids, and urea-promoted peroxidation. This work presents insights into developing optimal Anabaena culture for efficient operations of bioproduction and wastewater bioremediation with cyanobacteria.

Growth of freshwater cyanobacterium Aphanizomenon sp. ULC602 in different growing and nutrient conditions

“…Similarly, the growth rate of Anabaena sp. UTEX 2576 in the presence of sodium nitrate was shown to be higher than in media with urea or without a nitrogen source, and the Chlo-a accumulation of this bacterium in sodium nitrate medium was also 50% higher than the latter media (Norena-Caro et al, 2021). By contrast, the growing inhibition of urea was observed with Anabaena spiroides, which is comparable with our target bacterium (Volk and Phinney, 1968).…”

“…ULC602 was cultivated in a medium containing 50% dilution of the standard BG110 medium, indicating lower nutrient requirements for this strain compared to others. It was reported that the addition of N sources to the medium was demonstrated to improve the biomass of cyanobacteria (Cirés et al, 2017;Gour et al, 2018;Krausfeldt et al, 2019;Lu et al, 2019;Norena-Caro et al, 2021). The nitrogen can be supplied from atmospheric sources, from nitrates known as the popular form of dissolved inorganic nitrogen in aquatic systems, or from urea that is usually found in agro-industrial wastewaters (Glass et al, 2009); however, the presence of nitrogen sources from sodium nitrate (M10 medium) and urea (M11 medium) resulted in a decrease of the Chlo-a biomass produced by Aphanizomenon sp.…”

Biofilm Formation Influences the Wettability and Settling of Microplastics

“…20 Microbial Incubation. Alcanivorax was cultured in a modified marine broth, 21 and Synechococcus and Anabaena were cultured in a modified BG-11 medium 22 (see SI for details). The pH was 7.5 ± 0.2 for each medium.…”

EFFECTS OF VARIOUS LIGHT INTENSITIES ON PHYCOCYANIN COMPOSITION OF CYANOBACTERIUM LIMNOSPIRA FUSIFORMIS (VORONICHIN) NOWICKA-KRAWCZYK, MÜHLSTEINOVÁ & HAUER

“…Nutrients like nitrogen, phosphorus, calcium, magnesium, iron, manganese and light affect the pigment biosynthesis (Norena-Caro et al, 2021). Light is one of the most critical components that affect microalgae and cyanobacteria pigments production because it allows them to gather energy and carry out photosynthesis (Atta et al, 2013).…”