Nitrogen removal characteristics and potential application of the heterotrophic nitrifying-aerobic denitrifying bacteria Pseudomonas mendocina S16 and Enterobacter cloacae DS'5 isolated from aquaculture wastewater ponds

Shu, Hu; Sun, Hongwei; Huang, Wen; Zhao, Yang; Ma, Yu-Seung; Chen, Wei; Sun, Yujiao; Chen, Xiaoying; Zhong, Ping; Yang, Huirong; Wu, Xiaoping; Huang, Minwei; Liao, Sentai

doi:10.1016/j.biortech.2021.126541

“…It might occur in water samples in the high- and low-density systems in this study due to the accretion of shrimp commensals as biofloc are characterized by a zero-water exchange closed system. Enterobacter cloacae might be helpful in aquaculture water treatment and recorded with denitrification abilities [ 57 ]. The efficiencies of inorganic nitrogen removal of E. cloacae were 72.27 to 96.44%.…”

Section: Resultsmentioning

confidence: 99%

Assessment of Performance, Microbial Community, Bacterial Food Quality, and Gene Expression of Whiteleg Shrimp (Litopenaeus vannamei) Reared under Different Density Biofloc Systems

Said

¹

,

El-barbary

²

,

Ahmed

³

2022

Aquaculture Nutrition

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Biofloc shrimp culture, as a way of improving shrimp production, gains worldwide consideration. However, the effects of the biofloc system on shrimp culture at high densities could be a challenge. Here, this study is aimed at identifying a better stocking density of whiteleg shrimp (Litopenaeus vannamei) between two intensive biofloc systems of 100 and 300 org./m2. Achieving that was done by comparing growth performance, water quality, feed utilization, microbial loads from water and shrimps, and gene expression of growth, stress, and immune-related genes. Shrimp postlarvae with a mean weight of 35.4 ± 3.7 mg were reared in six indoor cement tanks (36 m3 total capacity each) at two stocking densities (3 replicates each) for a rearing period of 135 days. Better final weight, weight gain, average daily weight gain, specific growth rate, biomass increase percentage, and survival rate were associated with lower density (100/m2), whereas high-density showed significantly higher total biomass. Better feed utilization was found in the lower density treatment. Lower density treatment enhanced water quality parameters, including higher dissolved oxygen and lower nitrogenous wastes. Heterotrophic bacterial count in water samples was recorded as 5.28 ± 0.15 and 5.11 ± 0.28 log CFU/ml from the high- and low-density systems, respectively, with no significant difference. Beneficial bacteria such as Bacillus spp. were identified in water samples from both systems, still, the Vibrio-like count was developed in the higher density system. Regarding shrimp food bacterial quality, the total bacterial count in the shrimp was recorded as 5.09 ± 0.1 log CFU/g in the 300 org./m2 treatment compared to 4.75 ± 0.24 log CFU/g in the lower density. Escherichia coli was isolated from the shrimps in a lower density group while Aeromonas hydrophila and Citrobacter freundii were associated with shrimps from a higher density system. Immune-related genes including prophenoloxidase, superoxide dismutase (SOD), and lysozyme (LYZ) expressions were all significantly higher expressed in the shrimp from the lower density treatment. Toll receptor (LvToll), penaiedin4 (PEN4), and stress-related gene (HSP 70) showed a decreased gene expression in the shrimp raised in the lower density. Significant upregulation of growth-related gene (Ras-related protein-RAP) expression was associated with the lower stocking density system. In conclusion, the current study found that applying high stocking density (300 org./m2) contributes negatively to performance, water quality, microbial community, bacterial food quality, and gene expression of immune, stress, and growth-related genes when compared with the lower stocking density system (100 org./m2) under biofloc system.

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“…P. mendocina is a new type of aerobic bacteria, which has the advantages of assimilation and dissimilation while simultaneously efficiently removing nitrate [18]. A previous study isolated a strain of P. mendocina S16 from freshwater aquaculture ponds and showed significant heterotrophic nitrification-aerobic denitrification abilities, which indicated the potential application of the strain in aquaculture tail water treatment [19]. Another study isolated two P. mendocina strains from a healthy Anas platyrhynchos fecal sample (Ps542) and lettuce (Ps799), and whole genome analysis showed that both strains lacked antimicrobial-resistance genes, but the Ps799 genome showed a MOBP3 family relaxase; however, it possessed an important number of virulence factors, including flagella, pili, leukotoxin, and the Type 2 and Type 6 Secretion Systems, which could be responsible for their pathogenesis [20].…”

Section: Discussionmentioning

confidence: 99%

The characteristics of the novel bacterial strain Pseudomonas mendocina isolated from freshwater aquaculture farm

Kai Yan,

Shuai Wei,

Haifeng Wang

2024

Cell Mol Biol (Noisy-le-grand)

0

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Plastic contamination can cause damage to the water quality of fish farm ponds, and also affect the quality of the final product. Pseudomonas mendocina was found to biodegrade plastics. Our study aimed to investigate the physicochemical properties and drug resistance of P. mendocina isolated from local freshwater aquaculture farms. Firstly, the strain was isolated from aquaculture water and then identified by matrix-assisted flight mass spectrometry and 16S rDNA sequencing. Then, biochemical and antibiotic resistance analyses were performed, and a microbial high-throughput growth detector was used to assess the growth of the strain. Finally, PCR and proteomics analyses were conducted to determine drug-resistance-related genes/proteins. According to the results of the spectrum diagram and sequencing, the isolated bacteria were identified as P. mendocina, and were positive for reactions of ADH, MTE, LAC, MNE, FRU, CIT, MLT, ONPG, and ACE. P. mendocina was sensitive to most of the antibiotics, and its resistance to CHL, MIN, and TIC/CLA was intermediate. Additionally, gyrB was the resistance gene, and mdtA2, mdtA3, mdaB, and emrK1 were closely related to the drug resistance of P. mendocina. Our results show the biochemical properties of P. mendocina in isolated aquaculture water, and provide a new perspective for P. mendocina involved in the biological removal of plastics or microplastics in freshwater aquaculture farms.

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“…Enterobacter cloacae have a potential application in aquaculture water treatment. Shu et al [56] reported E. cloacae isolation from aquaculture water and showed significant heterotrophic denitrification ability. The efficiencies of inorganic nitrogen removal by E. cloacae were 72.27 to 96.44%.…”

Section: Proximate Composition Of Bioflocsmentioning

confidence: 99%

Carbohydrate Supplement Impact on Growth Performance, Bacterial Community, and Bacterial Food Quality of Whiteleg Shrimp (Litopenaeus vannamei) under Biofloc System

Said

¹

,

Ahmed

²

2022

Aquaculture Nutrition

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Biofloc technology has a high impact on enhancing shrimp production. Suitable supplemented carbohydrates (CHO) could affect the type of microorganisms developed in the system, which reflects on shrimp production, food safety, and public health. Here, we aimed to compare the effects of sugarcane molasses and wheat flour as carbohydrate sources on biofloc technology. That was achieved through measuring the following parameters: water quality, growth performance, feed utilization, floc composition, shrimp whole body composition, microbial community, and biofloc shrimp food bacterial quality. Postlarvae of whiteleg shrimp (Litopenaeus vannamei) with a mean weight ( 38.47 ± 5.8 mg ) were stocked at a density of 200 individuals/m2 and cultured under a biofloc system for 128 days in six tanks with a total water volume of 30 m2 each. Water quality analysis revealed a better-dissolved oxygen concentration (5.59 mg/L) in the wheat flour treatment, whereas no significant differences were found between the two treatments in ammonia, nitrite, and pH levels. Increased turbidity (64.27 NTU) and floc volume (18.40 mL/L) were recorded with molasses treatment. Growth performance including final weight, weight gain, average daily gain, weight gain per week, and specific growth rate (12.37 g, 12.34 g, 0.096 g/d, 0.68, and 4.70%, respectively) were all significantly higher in the molasses treatment. Wheat flour treatment was associated with a higher survival rate (99%), biomass (71.16 Kg), and biomass increase percentage (395.337) in shrimps. It also improved feed utilization in terms of a lower feed conversion ratio (1.37) and higher protein efficiency ratio (1.92). The chemical composition of biofloc and shrimp whole body were both nutritious higher in wheat flour treatment. In water, total heterotrophic bacterial counts with sugarcane molasses treatment and wheat flour were estimated as 3.4 × 10 5 CFU / mL and 1.2 × 10 5 CFU / mL , respectively, with no significant difference. In both treatments, beneficial bacteria such as lactic acid bacteria and Enterobacter cloacae were identified in water with the absence of pathogenic Vibrio spp. Wheat flour had a significantly lower total Vibrio-like count (TVC). Shrimps had lower TVC ( 1.9 × 10 4 CFU / g ) with flour than with molasses ( 1.32 × 10 5 CFU / g ). Cronobacter spp. were associated with shrimps in BFT supplemented with molasses, which might pose a potential risk to food safety. In conclusion, the use of wheat flour was the best for shrimp production and shrimp food bacterial quality.

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Nitrogen removal characteristics and potential application of the heterotrophic nitrifying-aerobic denitrifying bacteria Pseudomonas mendocina S16 and Enterobacter cloacae DS'5 isolated from aquaculture wastewater ponds

Cited by 36 publications

References 39 publications

Assessment of Performance, Microbial Community, Bacterial Food Quality, and Gene Expression of Whiteleg Shrimp (Litopenaeus vannamei) Reared under Different Density Biofloc Systems

Assessment of Performance, Microbial Community, Bacterial Food Quality, and Gene Expression of Whiteleg Shrimp (Litopenaeus vannamei) Reared under Different Density Biofloc Systems

The characteristics of the novel bacterial strain Pseudomonas mendocina isolated from freshwater aquaculture farm

Carbohydrate Supplement Impact on Growth Performance, Bacterial Community, and Bacterial Food Quality of Whiteleg Shrimp (Litopenaeus vannamei) under Biofloc System

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