Nitrogen removal and nitrogen functional gene abundances in three subsurface wastewater infiltration systems under different modes of aeration and influent C/N ratios

Pan, Jing; Qi, Shiyue; Sun, Yuchun; Jiang, Yingying; Zhao, Ning; Huang, Linli; Sun, Yining

doi:10.1016/j.biortech.2017.05.112

Cited by 60 publications

(22 citation statements)

References 7 publications

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“…However, compared to bioreactors with NO 3 − as the sole electron donor ( K value of 0.1195 h −1 ), the reduction rate of NO 3 − was significantly lower in denitrification bioreactors coexisting with Cr(VI). As Cr(VI) loading increased, the removal rate of NO 3 − decreased whereas NO 2 − increased, which could be due to the detrimental influences of Cr(VI) on the functional activities of the microorganisms through their entry into the cell and impacts on growth and metabolism . This hypothesis was supported by variations in the extracellular protein concentrations under different initial Cr(VI) concentrations (Fig.…”

Section: Resultsmentioning

confidence: 99%

Insights into simultaneous microbial chromium and nitrate reduction: inhibitory effects and molecular mechanisms

Chen

et al. 2019

J of Chemical Tech & Biotech

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BACKGROUND: The potential effect of chromium on the denitrification process has drawn much attention recently. The efficient remediation of groundwater contaminated by both hexavalent chromate [Cr(VI)] and nitrate (NO 3 − ) was hampered due to the deficiency of denitrification in the presence of Cr(VI). The goal of this work was to understand the mechanisms of how Cr(VI) affected denitrification. RESULTS:To better understand the underlying nature, Cr(VI) effects on denitrification were investigated in batch. Negative effects of Cr(VI) exposure on denitrification were confirmed and ascribed to restriction of bacterial activities, by regulating functional genes expression and altering community composition. Stronger inhibition of the expression of denitrifying genes was observed with increased Cr(VI) loading (0-50 mg L −1 ). The critical inhibitory concentration and IC 50 of Cr(VI) on electron transport system activities were calculated to be 3.21 and 4.87 mg L −1 , respectively. Further amplicon analysis revealed that Betaproteobacteria were enriched, implying their potential key role in simultaneously removing nitrate and Cr(VI). The presence of Cr(VI) also upregulated the metabolic activities related to apoptosis. CONCLUSION: These findings shed light on the biogeochemical fates of Cr(VI) and NO 3− in aquifers, and may contribute in enhancing their practical application for bioremediation using microbial processes. ETSA assayElectron transport activity of the bacteria was measured by reducing 2-(p-iodophenyl)-3-(p-nitrophenyl)-5-phenyl tetrazolium wileyonlinelibrary.com/jctb

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Section: Resultsmentioning

confidence: 99%

Insights into simultaneous microbial chromium and nitrate reduction: inhibitory effects and molecular mechanisms

Chen

et al. 2019

J of Chemical Tech & Biotech

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“…Therefore, operation modes such as aeration could create an aerobic environment and shunt distributing wastewater could serve as a carbon source to facilitate nitrogen removal. In recent years, nitrogen removal in SWI systems was attributed to nitrogen fixation, anaerobic ammonium oxidation, and aerobic denitrification, and the intricate relationships are shown in Figure (Ji, Zhi, & Tan, ; Pan et al., ). Additional research on the mechanism is needed for a better understanding to provide valuable insight into the overall function and structure of SWI systems.…”

Section: Discussionmentioning

confidence: 99%

“…The results indicated that TN removal increased by 13.32% in the shunt system and the NO 3 − –N concentration was approximately half that of the nonshunt system. The analysis of microbial populations also showed that shunt distributing wastewater significantly increased the number of denitrifying bacteria, which was more than 100 times that of the nonshunt system (Pan et al., ). Notably, although shunt distributing wastewater can ameliorate the denitrification of SWI systems, unoxidized organic matter and NH 4 + –N are usually generated in the shunting system due to the shorter HTR of shunt distributing wastewater (Tunçsiper, Ayaz, & Akça, ; Zou et al., ).…”

Section: Recognition Of Influential Factors and Concerns In Engineerimentioning

confidence: 99%

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The design and operation of subsurface wastewater infiltration systems for domestic wastewater

Wang

Chi

et al. 2019

Water Environment Research

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Throughout rural regions, large amounts of domestic wastewater are discharged into natural bodies of water without treatment. A well‐designed subsurface wastewater infiltration (SWI) system is an effective wastewater pollution removal strategy for small and remote communities due to its low energy consumption, low operational cost, and good performance. This paper describes the types of structures and purification mechanisms of SWI systems, focusing on the design of substrate materials and the optimization of different operation modes including the hydraulic loading rate, pollutant loading rate, intermittent operation, aeration, and shunting distribution. The challenges and trends in the development of SWI systems are also discussed. Practitioner points The construction and purification mechanisms of SWI system are described. The design of substrates and the optimization of operation modes are focused. The challenges and the development trends for the system are further introduced.

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“…To determine functional gene abundances, soil mixtures were sampled from sampling ports every 10 days and quantified by quantitative polymerase chain reaction (qPCR), according to Pan, Qi, Sun, Jiang, and Zhang (2017).…”

Section: Sample Collection and Analysismentioning

confidence: 99%

Effect of aeration and hydraulic loading rate on nitrogen removal by subsurface infiltration systems

Zheng

Huang

Pan

et al. 2019

Water Environment Research

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This study investigated the effect of hydraulic loading rate (HLR) on matrix dissolved oxygen (DO), organic matter removal, nitrogen removal, N2O emissions, and the abundances of functional genes participating in nitrogen removal in intermittent aerated mode (IAM) and nonaerated mode (NAM) subsurface infiltration systems (SISs). In contrast to NAM SISs, IAM SISs were able to create aerobic conditions in the upper matrix (above 50 cm depth) and anoxic or anaerobic conditions in the lower matrix (below 80 cm depth). Subsequently, this enhanced the abundance of functional genes related to nitrogen removal. Chemical oxygen demand (COD) and nitrogen removal performance were significantly higher under IAM SISs than with NAM SISs. Under a HLR of 0.3 m3/(m2 d), the IAM SIS was able to achieve low N2O emissions (12.6 mg/[m2 d]) along with removal efficiencies of 90.5%, 91.4%, and 85.7% for COD, ammonia nitrogen (NH4+‐N), and total nitrogen (TN), respectively. Practitioner points Intermittent aeration successfully realized sequential aerobic and anaerobic conditions at 50 cm depth and at 80 and 110 cm depths of a subsurface infiltration system. Intermittent aeration reduced N2O emissions and improved hydraulic loading rate and organic matter, nitrogen removal efficiencies. Intermittent aeration enhanced the abundances of amoA, nxrA, napA, narG, nirS, nirK, qnorB, and nosZ.

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Nitrogen removal and nitrogen functional gene abundances in three subsurface wastewater infiltration systems under different modes of aeration and influent C/N ratios

Cited by 60 publications

References 7 publications

Insights into simultaneous microbial chromium and nitrate reduction: inhibitory effects and molecular mechanisms

Insights into simultaneous microbial chromium and nitrate reduction: inhibitory effects and molecular mechanisms

The design and operation of subsurface wastewater infiltration systems for domestic wastewater

Effect of aeration and hydraulic loading rate on nitrogen removal by subsurface infiltration systems

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